1. In a disc diffusion susceptibility test, which of the following can result if discs are placed on the inoculated media and left at room temperature for an hour before incubation? a. the antibiotic would not diffuse in to the medium, resulting in no zone b. zones of smaller diameter would result c. zones of larger diameter would result d. there would be no effect on the final zone diameter 2. Urine reagent strips should be stored in a (n) : a. refrigerator ( 4 to 7 C ) b. incubator ( 37 C ) c. cool, dry area d. open jar exposed to air 3. Group B , B-hemolytic streptococci may be distinguished from other hemolytic streptococci by which of the following procedures ? a. lancefiled typing b. growth in 6.5 % NaCl broth c. growth on bile esculin medium d. bacitracin susceptibility 4. The stock culture needed for quality control testing of motility is: a. Salmonella typhimurium / Escherichia coli b. Escherichia coli / Pseudomonas aeruginosa c. Serratia marcescens / Escherichia coli d. Shigella sonnei / Escherichia coli 5. An unusual number of methicillin-resistant Staphylococcus aureus (determined by the Kirby-Bauer method) were isolated in the laboratory in the past month. Which of the following is the most likely explanation? a. incubation of the susceptibility plates at 35 C b. deterioration of the methicillin discs c. inoculation of plates 10 minutes after standardizing the inoculum d. standardization of the inoculum to 0.5 McFarland turbidity standard 6. Blood collected in EDTA undergoes which of the following changes if kept at room temperature for 6 – 24 hours. a. increased hematocrit and MCV b. increased sedimentation rate and MCV c. increased MCHC and MCV d. decreased Reticulocyte count and hematocrit 7. A total leukocyte count is 10,000/ ML and 25 NRBCs are seen per 100 leukocytes on the differential. What is the corrected leukocyte count? a. 2,000/ ML b. 8,000/ ML c. 10,000/ ML d. 12,000/ ML 8. The prothrombin time test required that the patient's citrated plasma be combined with: a. platelet lipids b. thromboplastin c. Ca++ and platelet lipids d. Ca++ and thromboplastin 9. A 56- years-old man was admitted to the hospital for treatment of a bleeding ulcer. The following laboratory data where obtained: RBC 4.2 * 1012 / L Serum iron 40 Mg/dl Hct 30% TIBC 460 Mg/dl Hgb 8.5 gm/dl Serum ferritin 12 mg/ml 9 WBC 5.0 * 10 /L Examination of the bone marrow revealed the absence of iron stores . there data are most consistent with which of the following conditions ? a. iron deficiency anemia b. anemia of chronic disease c. hemochromatosis d. acute blood loss 10. What is the mean corpuscular volume ( MCV ) if the hematocrit is 20% , the RBC is 1,500,000/ML , and the hemoglobin is 6 gm/dl ? a. 68 fl b. 75 fl c. 115 fl d. 133 fl 11. The following result was obtained in a Creatinine clearance evaluation: Urine concentration 0.084 gm/dl (7.43 mmol / L) Urine volume 1440 ml/24 hr Serum concentration 1.4 mg/ dl (124 Mmol /L) Body surface area 1.60 m2 (average = 1.73 m2 The Creatinine clearance in Ml / min is: a. 0.006 b. 0.022 c. 0.60 d. 64.9 12. The serum glucose concentration of a specimen from a diabetic patient undergoing a two-hour glucose tolerance test should return to the baseline (fasting level) after a minimum of: a 30 minutes b. 60 minutes c. 90 minutes d. 120 minutes 13. A colorimetric method calls for the use of 0.1 Ml serum , 5 Ml of reagent, and 4.9 Ml of water. What is the dilution of the serum in the final solution? a. 1 to 5 b. 1 to 10 c. 1 to 50 d. 1 to 100 14. Which of the following is the formula for calculating the dilution of a solution? ( V = volum , C = concentration ) a. V1 + C1 = V2 + C2 b. V1 + C2 = V2 + C1 c. V1 * C1 = V2 * C2 d. V1 * V2 = V1 * C2 15. Which of the following is the formula for calculating the unknown concentration based on Beers law? ( A = absorbance , C = concentration ) a. ( A unknown ) * C standard ( A standard ) b. C standard X A unknown c. A standard X A unknown d. ( C standard ) X 100 ( A standard ) 16. Which of the following statements about ABO-hemolytic disease of the newborn are true? 1. it usually requires an exchange transfusion 2. it can occur in the first born 3. it frequently results in stillbirth 4. it is frequently seen in group O mothers a. only 1,2 and 3 are correct b. only 1 and 3 are correct c. only 4 is correct d. all are correct 17. A unit of blood that has been out of your control for ten minutes, without being refrigerated, should be: 1. Cultured for bacterial contamination 2. Inspected for container closure 3. Stored at room temperature 4. Recorded to indicate return a. only 1,2 and 3 are correct b. only 1 and 3 are correct c. only 4 is correct d. all are correct For questions 18 and 19 Refer to the illustration below: 18. The egg depicted above is most likely to be found in a. Stool b. Saliva c. Sputum d. Urine 19. This Ova belong to the Parasite: a. Teania saginata b. Teania solium c. Enterobious vermicularis. d. Hymenolepis nana For questions 20 and 21 refer to the illustration below: 20. The organism depicted is a ( n ) : a. amoeba b. flagellate c. trypanosoma d. sporozoan 21. A 24- year – old woman who just returned from vacationing in Russia became ill with urinary tract infection. The above organism was found in her urine . The patient most likely is suffering from: a. giardiasis b. amebiasis c. ascariasis d. trichomniasis. 22. Which immunoglobulin is associated with allergic reactions? a. IgA b. IgE c. IgG d. IgM 23. A rapid indirect latex slide test showed no agglutination and was reported as negative on a woman who , on physical examination , was thought to be pregnant . a possible reason for the negative test result is : a. urine glucose was increased b. misinterpretation of the test c. urine specific gravity was greater than 1.015 d. urine protein was greater than 100 mg/dl 24. In systemic lupus erythematosus. High levels of which of the following antibodies are seen? a. anti- mitochondrial b. anti- smooth muscle c. anti- DNA d. anti- parietal cell 25. An RNA –containing virus is: a. HAV b. HBV c. CMV d. HSV 26. The lipid component which measurement would be the most severely affected by analysis of a non – fasting specimen is a) Triglycerides b) Cholesterol c) Fatty acids d) Apolipoprotein 27. A patient with total protein 6.1g/dl, albumin of 3.2 g/dl then the albumin / globulin (A/G) ratio is a) 3.2 / 6.1 b) 2.9 /6.1 c) 3.2 /2.9 d) 2.9 / 3.2 28. One of the following doesn’t belong to indirect bilirubin : a) Unconjugated b) Water – insoluble c) Cross blood brain barrier d) Secreted by the kidney 29. CA marker used in monitoring the course of the disease in patients with invasive epithelial of ovarian cancer is a) CA15-3 b) CA19-9 c) CA125 d) CA72-4 30. Glycosylated hemoglobin monitors the diabetic patient's blood glucose is a) HbA2 b) HbS c) HbA1c d) HbC 31. The extent to which measurements agree with the true value of the quantity being measured is known as a) Acceptable limits b) Accuracy c) Precision d) Reliability 32. Hemoglobin electrophoresis has greatest diagnostic value in : a) G6PHD deficiency & vit. K b) Hemoglobinopathies deficency c) In cases of hemoglobin anemias d) Hereditary spherocytosis 33. Which of the following antigens is the (backbone) from which all ABO antigens are made? a) Rh b) O c) H d) Se 34. Which of the following is not a type of granulocytes a) Neutrophils b) Monocytes c) Eosinophils d) Basophils 35. PT is a clinical test used to assess a) liver damage b) the extrinsic thrompoplastin system c) potential vitamin K deficiency d) all of the above 36. Which of the following blood product is the most likely to be contaminated by bacteria a) apheresis plateletes b) RBCs c) fresh frozen plasma d) pooled platelets concentrate 37. The results for blood group done by the reverse method are shown in the following table. The blood group is Test cells A1 B Patient serum ++++ ++++ a) AB b) O c) A d) B 38. In order to be called fresh frozen plasma (FFP), the plasma portion of whole blood must be placed at what temperature within what time limit? a) less than -8 Co in less than 18 hr b) less than -10 Co in less than 10 hr c) less than -18 Co in less than 8 hr d) less than -18 Co in less than 24 hr 39. Prior to tissue transplantation , the first laboratory test done a) ABO compatibility b)Immunoglobulins level c) HLA compatibility d) Non of above 40. Cryoprecipitate is prepared by a) Fractionation of plasma b) Filtration of fresh frozen plasma c) Controlled thawing of fresh frozen plasma d) Apheresis 41. Streptolysin O is a bacterial toxin produced by strains of a) Streptococcus pnemoniae b) Streptococcus pyogenes c)Streptococcus veridans d) B+C 42. The criteria for differentiation between viral & bacterial meningitis a) High protein level b) Low glucose level c) Type of cells d) All of the above 43. Selenite F Broth is an enrichment liquid medium for the isolation of a) Streptococcus fecalis b) Salmonella c) Pseudomonas aeruginosa d) Nocardia 44. For microbiological studies the most reliable urine sample is a) First void b) Mid – stream c) Last void d) Non of the above 45. In Gram stain procedure, safranin is applied immediately a) after the iodine b) after the crystal violet c) after the decolorizer d) before the iodine 46. All the following crystals are found in acid urine except a) Amorphous phosphates b) Uric acid c) Amorphous urates d) Calcium oxalate 47. Which of the following members of genus Candida is considered to be pathogenic for man? a) C.tropicalis b) C.albicans c) C.parakruzei d) C.guilliermondi 48. Visceral leishmaniasis (kala Azar ) is caused by a) L.tropica b) L.donovani c) L.braziliensis d) L.mexicana 49. The flagellate parasite which can be encountered in urine and vaginal secretions is a) Trichomonas hominis b) Trichomonas vaginalis c) Balantidium coli d) Trichuris trichura 50. Hydatid cyst disease is caused by a) Dipylidium caninum b) Taenia solium c) Echinococcus granulosus d) Hymenolepis diminuta 51. All of the following result are confirmatory of Diabetes Mellitus, except: A- FBG > 140 mg/dl B- 2hrs PPBG >200 mg/dl. C- 1hr PPBG > 200 mg/dl. D – Urine glcose > 250 mg/dl. 52. Adult male with the following results: Hct = 30%, Reticulocytes production index is: A – 1.5. B – 2.5 C – 3.5 D – 4.5 53. The hemogram parameter that can discriminate between IDA and B- thalassemia trait is : A – MCV. B – MCHC C – RDW D – MCH 54. The liver function test that indicates (Bile Duct Obstruction) is: A – High ALT B – High Alkaline phosphatase. C- Low Albumin. D – High Indirect Bilirubin. 55. The normal rang of Band Neutrophils in peripheral blood of adult male is : A – 0 – 2% B–2–6% C – 6 – 8% D – 40 – 70% 56. The Key test to differentiate between enterobacteriaceae and pseudomonas is A- The growth on Mackonky agar. B – Oxidase C - Glucose fermentation . D – Lactose fermentation. 57. The chemical substance that used in direct wet mount preparation for fungal identification is : A – Normal Saline. B – Alcohol 70 %. C – KOH 15% . D – H2O2 3%. 58. The result of ASOT following panel is: Dilution Direct Reaction Positive 1:1 Positive 1:2 Positive 1:4 Positive 1:8 1 : 16 Negative Negative A – 400 U/L B – 800 U/L C – 1600 U/L D – 3200 U/L 59. The most sensitive marker for alcoholic liver disease is: A – Alkaline phosphatase. B – AST . C – GGT . D – LDH . 60. The index of RBCs chromia is: A – Hb . B – MCV . C – MCH . D – MCHC . 61. The parameter that reflects the transferring level is : A – Serum Iron . B – TIBC . C – Ferritin . D – UIBC . 62. The following results : T3 : High , T4 High , TSH : High , indicate: A – Primary hypothyroidism B – Secondary hypothyroidism . C – Primary hyperthyroidism . D – Secondary hyperthyroidism . 63. To prepare 10 ml 0.1 M (NaOH) from 0.25 M (NaOH), the volume of 0.25 M (NaOH ) that should be used is : A – 1 ml B – 2 ml C – 4 ml D – 6 ml 64. If Blood Urea = 55.0 mg/ dl , the BUN should be reported as : A – 22.1 B – 36.6 . C – 25.7 . D – 117.7 . 65. The solution that used for manual platelets count is : A – Ammonium Oxalate 1 %. B – Trisoduim citrate 3.8%. C – Acetic Acid 3%. D – Sodium chloride 9%. 66. The Blood film shown in this picture is characterized by: A – Microcytic hyperchromic RBCs . B – Macrocytic hypochromic RBCs . C – Microcytic hypochromic RBCs . D – Macrocytic hyperchromic RBCs . 67. The most probable Bacteria that causes this pattern of growth on Blood agar is : A – Enterococcus faecalis . B – Eschenchia coli . C – Proteus species . D – Staphylococcus aureus . 68. The picture seen here is : A – Giardia Lamblia cyst . B – Trichuris trichuri Ova. C – Food artifact . D – Entamoeba histolytica cyst. 69. The ASOT is raised in infection caused by: A – Streptococcus sangins . B – Streptococcus pneumoniae C – Streptococcus pyogens D – Streptococcus bovis . 70. The Urinary crystals that appear in this picture are : A- Calcium Oxalate Crystals B- Uric acid Crystals. C- Cholesterol Crystals D-Triple Phosphate Crystals 71. Blood collected in EDTA undergoes which of the following changes if kept at room temperature for 6 – 24 hours. a) increased hematocrit and MCV b) increased sedimentation rate and MCV c) increased MCHC and MCV d) decreased Reticulocyte count and hematocrit 72. A total leukocyte count is 10,000/ L and 25 NRBCs are seen per 100 leukocytes on the differential. What is the corrected leukocyte count? a) 2,000/ ML b) 8,000/ ML c) 10,000/ ML d) 12,000/ ML 73. The prothrombin time test required that the patient's citrated plasma be combined with: a) platelet lipids c) Ca++ and platelet lipids b) thromboplastin d) Ca++ and thromboplastin 74. A 56- years-old man was admitted to the hospital for treatment of a bleeding ulcer. The following laboratory data where obtained: RBC 4.2 * 1012 / L Serum iron 40 mg/dl Hct 30% TIBC 460 mg/dl Hgb 8.5 gm/dl Serum ferritin 12 mg/ml WBC 5.0 * 109 /L Examination of the bone marrow revealed the absence of iron stores, these data are most consistent with which of the following conditions ? a) iron deficiency anemia b) anemia of chronic disease c) hemochromatosis d) acute blood loss 75. What is the mean corpuscular volume ( MCV ) if the hematocrit is 20% , the RBC is 1,500,000/L , and the hemoglobin is 6 gm/dl ? a) 68 fl b) 75 fl c) 115 fl d) 133 fl 76. The following result was obtained in a Creatinine clearance evaluation: Urine concentration 0.084 gm/dl (7.43 mmol / L) Urine volume 1440 ml/24 hr Serum concentration 1.4 mg/ dl (124 mmol /L) Body surface area 1.60 m2 (average = 1.73 m2 The Creatinine clearance in ml / min is: a) 0.006 b) 0.022 c) 0.60 d) 64.9 77. The serum glucose concentration of a specimen from a diabetic patient undergoing a two-hour glucose tolerance test should return to the baseline (fasting level) after a minimum of: a) 30 minutes c) 90 minutes b) 60 minutes d) 120 minutes 78. A colorimetric method calls for the use of 0.1 ml serum , 5 ml of reagent, and 4.9 ml of water. What is the dilution of the serum in the final solution? a) 1 to 5 c) 1 to 50 b) 1 to 10 d) 1 to 100 79. Which of the following is the formula for calculating the dilution of a solution? ( V = volume , C = concentration ) a) V1 + C1 = V2 + C2 b) V1 + C2 = V2 + C1 c) V1 * C1 = V2 * C2 d) V1 * V2 = V1 * C2 80. Which of the following is the formula for calculating the unknown concentration based on Beers law? ( A = absorbance , C = concentration ) a) ( A unknown ) * C standard ( A standard ) b) C standard X A unknown c) A standard X A unknown d) ( C standard ) X 100 ( A standard ) 81. Which of the following statements about ABO-hemolytic disease of the newborn is true? 1. it usually requires an exchange transfusion 2. it can occur in the first born 3. it frequently results in stillbirth 4. it is frequently seen in group O mothers a) only 1,2 and 3 are correct c) only 4 is correct b) only 1 and 3 are correct d) all are correct 82. A unit of blood that has been out of your control for ten minutes, without being refrigerated, should be: 1. Cultured for bacterial contamination 2. Inspected for container closure 3. Stored at room temperature 4. Recorded to indicate return a) only 1,2 and 3 are correct c) only 4 is correct b) only 1 and 3 are correct d) all are correct For questions 43 and 44 refer to the illustration below: 83. The egg depicted above is most likely to be found in a) Stool c) Sputum b) Saliva d) Urine 84. This ova belongs to the parasite: a) Teania saginata c) Enterobious vermicularis. b) Teania solium d) Hymenolepis nana For questions 45 and 46 refer to the illustration below: 85. The organism depicted is a ( n ) a) amoeba c) trypanosoma b) flagellate d) sporozoan 86.The above organism was found in the urine sample of a 24- years – old woman. This patient is most likely suffering from: a) giardiasis c) ascariasis b) amebiasis d) trichomniasis 87. Which immunoglobulin is associated with allergic reactions? a) IgA c) IgG b) IgE d) IgM 88. A rapid indirect pregnancy latex slide test showed no agglutination and was reported as negative on a woman who , on physical examination , was thought to be pregnant . A possible reason for the negative test result is: a) urine glucose was increased b) misinterpretation of the test c) urine specific gravity 1.015 d) urine protein 100 mg/dl 89. In systemic lupus erythematosus, high levels of …………..antibodies are seen a) anti- mitochondrial c) anti- DNA b) anti- smooth muscle d) anti- parietal cell 90. An RNA –containing virus is: a) HAV c) CMV b) HBV d) HSV 91. A patients' blood group results done by the reverse method are shown in the following table, his blood group is Test cells A1 B Patient serum ++++ ++++ a) AB c) A b) O d) B 92. Which of the following blood product is the most likely to be contaminated by bacteria a) apheresis plateletes c) fresh frozen plasma b) RBCs d) pooled platelets concentrate 93. Prior to tissue transplantation, the first laboratory test done a) ABO compatibility c) HLA compatibility b)Immunoglobulins level d) Non of above 94. The immunoglobulin IgM has the following characteristics except a)secreted in the early stages of a primary infection c) pentamer b) 900,000 dalton Mwt d) cross the placenta 95. One of the following HBV markers indicates that the patient is infectious a) HbsAg c) HbcAg b)HbeAg d) Anti Hbe 96. Streptolysin O is a bacterial toxin produced by strains of a) Streptococcus pnemoniae c)Streptococcus Viridans 97. The criteria for differentiation between viral & bacterial meningitis a) High protein level c) Type of cells 98. b) Streptococcus pyogenes d) B+C b) Low glucose level d) All of the above For microbiological studies the most reliable urine sample is a) First void b) Mid – stream c) Last void d) Non of the above 99. Biochemical tests for identification of bacteria are used to determine a) enzymatic activities c) amino acid sequences b) nucleic acid base composition d) staining characteristic 100. All the following crystals are found in acid urine except a) Amorphous phosphates c) Amorphous urates b) Uric acid d) Calcium oxalate .