Supporting Information
Facile Aqueous Synthesis and Stabilization of Nearly Monodispersed Gold
Nanospheres by Poly(L-proline)
Manos Gkikas,a,b,c Jaakko Timonen,c,d Janne Ruokolainen,c Paschalis Alexandridisb and Hermis Iatroua*
a
Chemistry Department, University of Athens, Panepistimiopolis, Zografou, 15771, Athens, Greece
b
Department of Chemical and Biological Engineering, University at Buffalo, The State University of New
York, Buffalo, NY 14260-4200, USA
c
Molecular Materials, Department of Applied Physics, Aalto University School of Science and
Technology (previously Helsinki University of Technology), 00076 Aalto, Finland
d
Debye Institute for Nanomaterials Science, Utrecht University, P.O. Box 80.051, NL-3508 TB Utrecht, The
Netherlands
Figure S1. TEM images of the Au-NPs synthesized at 50oC by monomer BocLP-Au and 2 mM Au(III) at
molar ratio 70:1 after 1 day.
d =11.9 nm ± 3.2
Figure S2. TEM images of PLP-Au nanospheres synthesized at 50oC by PLP 7.4 x 103 g/mol and 2 mM
Au(III) at molar ratio 2:1 after 1 day.
d =13.7 nm ± 2.6
Figure S3. TEM images of PLP-Au nanospheres synthesized at 50oC by PLP 9.3 x 103 g/mol and 1 mM
Au(III) at molar ratio 2:1 after 1 day.
Table 1 . Influence of temperature and PLP/Au molar ratio to the size of the formed Au-NPs.
Polymer
Mn x 103
(g·mole-1)a
[AuIII]
(mM)
molar ratio
[AuIII]/[PLP]
T
( C)
CPLP
(mM)
d Au-PLP after
d Au-PLP after
1 hour (nm)
1 day (nm)
b
25
0.4
8.3
multishaped
o
PLP
4.6
2.0
1:2
PLP
4.6
2.0
1:2
50
0.4
5.4
9.6
PLP
4.6
2.0
1:2
65
0.4
6.8
7.0
PLP
4.6
2.0
1:5
50
1.1
-
7.1
PLP
4.6
0.25
1:4
50
0.1
-
7.5
PLP
4.6
0.5
1:2
50
0.1
-
19.6
PLP
4.6
1.0
1:1
50
0.1
-
5.5
a
By SEC-TALLS in water (0.1 M NaNO3) / ACN 80:20 at 35 °C.
b
Molar ratio of Au(III) to the moles of polymeric chains.
d =7.0nm ± 2.8
Figure S4. TEM image of PLP-Au nanospheres synthesized at 65oC by PLP 4.6 x 103 g/mol and 2 mM Au(III)
at molar ratio 2:1 after 1 day.
d =5.5 nm ± 3.0
Figure S5. TEM images of PLP-Au nanospheres synthesized at 50oC by stable concentration of PLP 4.6 x
103 g/mol and 1.0 mM Au(III) at molar ratio 1:1 after 1 day.
Figure S6. 1H-NMR spectra of PLP-Au nanoprticles in CF3COOD synthesized at 50oC by using PLP 4.6 x 103
g/mol and 2 mM Au(III), showing the formation of alcohols to the ring Cδ of PLP.
Figure S7. CD spectra showing a slight shift in the negative band of the PLP-Au nanoparticles formed at
50oC by using PLP 4.6 x 103 g/mol and 2 mM Au (III), diluted to the appropriate concentration.