C-6 Name: ______________________________ SCIENCE – The Cell February 2008 Section #/color: ___ / _________ TRIO TEAM CHALLENGE Since your team has the advantage of having THREE people to pull the same weight as the other teams of only two, you have the assignment to take on the TRIO TEAM CHALLENGE. You see, there are really THREE general groups of cells: PLANT, ANIMAL, and BACTERIAL. One person in your group – one that is willing to take on the challenge of learning something extra – will be making the bacterial cell model in addition to the plant and animal cell models that all the other duo teams are doing! Here is information to start your extra-challenge on an easier course: Bacterial Cell Structure Nucleoid DNA Ribosomes Cell wall Plasma membrane Outer membrane Capsule Flagella Pili Internal Structure: Bacteria have a very simple internal structure, and no membrane-bound organelles. nucleoid DNA in the bacterial cell is generally confined to this central region. Though it isn't bounded by a membrane, it is visibly distinct (by transmission microscopy) from the rest of the cell interior. ribosomes Ribosomes give the cytoplasm of bacteria a granular appearance in electron micrographs. Though smaller than the ribosomes in eukaryotic cells, these inclusions have a similar function in translating the genetic message in messenger RNA into the production of peptide sequences (proteins). storage granules (not shown) Nutrients and reserves may be stored in the cytoplasm in the form of glycogen, lipids, polyphosphate, or in some cases, sulfur or nitrogen. (not shown) Some bacteria, like Clostridium botulinum, form spores that are highly resistant to drought, high temperature and other endospore environmental hazards. Once the hazard is removed, the spore germinates to create a new population. Surface Structure: Beginning from the outermost structure and moving inward, bacteria have some or all of the following structures: capsule This layer of polysaccharide (sometimes proteins) protects the bacterial cell and is often associated with pathogenic bacteria because it serves as a barrier against phagocytosis by white blood cells. outer membrane (not shown) This lipid bilayer is found in Gram negative bacteria and is the source of lipopolysaccharide (LPS) in these bacteria. LPS is toxic and turns on the immune system of , but not in Gram positive bacteria. cell wall Composed of peptidoglycan (polysaccharides + protein), the cell wall maintains the overall shape of a bacterial cell. The three primary shapes in bacteria are coccus (spherical), bacillus (rodshaped) and spirillum (spiral). Mycoplasma are bacteria that have no cell wall and therefore have no definite shape. (not shown) This cellular compartment is found only in those bacteria that have both an outer periplasmic membrane and plasma membrane (e.g. Gram negative bacteria). In the space are enzymes and space other proteins that help digest and move nutrients into the cell. plasma membrane This is a lipid bilayer much like the cytoplasmic (plasma) membrane of other cells. There are numerous proteins moving within or upon this layer that are primarily responsible for transport of ions, nutrients and waste across the membrane. Appendages: Bacteria may have the following appendages: pili These are hollow, hairlike structures made of protein allow bacteria to attach to other cells. A specialized pilus, the sex pilus, allows the transfer from one bacterial cell to another. Pili (sing., pilus) are also called fimbriae (sing., fimbria). The purpose of flagella (sing., flagellum) is motility. Flagella are long appendages which rotate by means flagella of a "motor" located just under the cytoplasmic membrane. Bacteria may have one, a few, or many flagella in different positions on the cell.