Antibody Production
PAb 8-week Project Information
1st BASE Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II, Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: antibody-sg@base-asia.com
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: antibody-my@base-asia.com
Date:
Name of Researcher:
Address:
Email:
Contact No.
Purchase Order Number:
Project Name:
Please choose preferred package: PAb 8-week Package 1, 2, 3, 4 (see overleaf for details)
Immunogen Details:
*Immunogen provided by Researcher (for Package 1 only)
Protein / Peptide Size:
kDa
Amount:
mg
Form of Protein:
Solution (Concentration:
mg/ml) (Buffer contains:
)
Lyophilised
Gel Strip
* Note: 4 mg of soluble Immunogen required for Affinity Purification and 1 mg for each ELISA Testing (2 animals)
Additional Services:
Conjugation of Immunogen to KLH carrier protein
Note: 3mg of antigen in 1xPBS required (min. of 1mg/ml)
Peptide Synthesized by 1st BASE (included in Package 2, 3 and 4)
Note:
a) FREE up to 15 residues, > 70% purity for Package 2, 3 and 4
b) Recommended < 30 residues for other Custom Package
c) Allow 2 weeks of synthesis
Sequence: NH2 -
-OH
Optional purification of peptides:
Purity > 80% by HPLC (charges applied)
Purity > 95% by HPLC (charges applied)
Shipment Information:
Use 1st BASE’s Fedex Account
Charge to my FedEx Account (No.
)
Preference:
Ship 2ml Aliquots of Bleeds 1 & 2 (note: additional charges may apply)
Ship after Bleed 2 & at end of project (total of 2 shipments) (note: additional charges may apply)
Upon Project Completion
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Custom PAb Packages and Services
Please
Tick
Package
Details
Remarks
PAb 8week
Package
1
Polyclonal Antibody Production - 2 Rabbits
(*Immunogen provided by researcher)
Includes:
-Immunization and anti-sera production (75ml/rabbit)
-ELISA Testing for Bleed 1
*2mg of
antigen in
1xPBS
preferred
(min. of
1mg/ml)
PAb 8week
Package
2
Please
Tick
Additional
Services:
Details
Remarks
ELISA Testing
and Reporting
(additional)
ELISA Testing and Reporting, on top of
ELISA on Bleed 1 (which already included in
Packages 1 to 4)
Please indicate which Bleed? 2, 3
0.4mg
soluble
antigen
per ELISA
Polyclonal Antibody Production - 2 Rabbits
Includes:
-Peptide synthesis (15aa), Purification (> 70% Purity)
and Conjugation
-Immunization and anti-sera production (75ml/rabbit)
-ELISA Testing for Bleed 1
Protein A / G
Purification
(25ml)
Protein A/G Purification (25ml of purified
anti-sera).
Please state which bleed? 1, 2, 3
PAb 8week
Package
3
Polyclonal Antibody Production - 2 Rabbits
Includes:
-Peptide synthesis (15aa), Purification (> 70% Purity)
Contact us
and Conjugation
-Immunization and anti-sera production (62.5mL/rabbit) for a free
peptide
-Affinity Purification on Bleed 3 for 12.5mL/ rabbit
antigenicity
(includes reusable column)
analysis!
-ELISA Testing on Bleed 1
Affinity
Purification
(25ml)
Affinity Column Preparation and
Purification of Bleeds (25ml of purified
anti-sera) .
Please state which bleed? 1, 2, 3
PAb 8week
Package
4
Polyclonal Antibody Production - 2 Rabbits
Includes:
-Peptide synthesis, Purification (> 70% Purity)
and Conjugation
- Immunization and anti-sera production
- Large scale Affinity Purification for all Bleeds
(75mL/ rabbit)
- ELISA Testing on Bleed 1
Large scale
Affinity
Purification
Large scale Affinity Column Preparation
and Purification (150-200ml of purified
serum)
Rabbit
Exsanguination
Exsanguination of Animals for Project
Termination
(Terminal bleed will be about 60ml
per animal)
Project Extension (28 days) includes
-1 Additional Boost and
-2 Bleeds (25ml each) for 2 rabbits
Screening of PAb serum on a choice of
IHC tissue panel.
Project
Extension
Custom
Antibody
Screening-IHC
Custom
Antibody
Screening-WB
Min. 3mg
of soluble
antigen
required
Screening of PAb serum on choice of
14 Western Blot lysates
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