SUPPORTING INFORMATION Tree line identification from pollen data: beyond the limit? H.A. Binney, P.W. Gething, J.M. Nield, S. Sugita and M.E. Edwards Journal of Biogeography Appendix S2 Description of characteristic taxa of biomes used in this study (Table S1) and comparison of the overall percentage correct match for pollen biomes versus vegetation for Canada (Table S2) and Alaska (Table S3). Table S1 Description of characteristic taxa of biomes used in this study (from Bigelow et al., 2003). Biome name Characteristic Species or Assemblages in Northern Hemisphere Arctic Cushion forb tundra Saxifragaceae, Caryophyllaceae, Papaver, Draba Graminoid and forb tundra Artemisia, Kobresia, Brassicaeae, Asteraceae, Caryophyllaceae, grass, true mosses Prostrate dwarf-shrub tundra Salix, Dryas, Pedicularlis, Asteraceae, Brassicaceae, grass and sedge Erect dwarf-shrub tundra Betula, Salix Vaccinium, Empetrum,, Cassiope, grass and sedge Low– and high-shrub tundra Alnus, Betula, Salix, Pinus pumila, Eriophorum, Sphagnum Cold deciduous forest Larix, Betula or Populus subg. tremula/tremuloides, with Pinus pumila Cold evergreen needleleaved forest Picea and Abies, with some Pinus, Betula, Populus or Larix Cool evergreen needleleaved forest Pinus strobus, Pinus resinosa, Picea glauca, Pinus banksiana, Abies balsamea, Acer saccharum Cool mixed forest Picea, Pinus, Quercus, Acer, Ulmus Table S2 Comparison of the overall percentage correct match for pollen biomes versus vegetation for i) all Canadian sites and ii) the Canadian lake-only sites. Column 3 shows error due to tundra vegetation shown as forest by the pollen. The opposite error (forest shown as tundra by pollen) is 100 minus the value in column 3. Biomization method i) All Canadian sites α 4:1:0.25 √ α 4:1:0.25 no √ No Pinus or factors with √ α 3:1:0.33 √ α 4:1:0.25 with C & √ α 3:1:0.33 no √ α 3:1:0.33 with C & √ α 3:1:0.5 √ α 4:1:0.25 with C & no √ α 2:1:0.5 √ α 3:1:0.5 no √ α 3:1:0.5 with C & √ α 3:1:0.33 with C & no √ α 2:1:0.5 no √ α 2:1:0.5 with C & √ α 2:1:0.5 with C & no √ α 3:1:0.5 with C & no √ Original PAIN C only with √ C only with no √ Biomization method ii) Canadian lake-only sites α 4:1:0.25 √ α 4:1:0.25 no √ No Pinus or factors with √ α 3:1:0.33 √ α 4:1:0.25 with C & √ α 3:1:0.33 no √ α 3:1:0.33 with C & √ α 3:1:0.5 √ α 4:1:0.25 with C & no √ α 2:1:0.5 √ α 3:1:0.5 no √ α 3:1:0.5 with C & √ α 3:1:0.33 with C & no √ α 2:1:0.5 no √ α 2:1:0.5 with C & √ Overall success rate % Tundra vegetation shown as forest by the pollen (%) 67.1 66.8 64.7 64.1 63.4 62.0 61.4 60.7 59.0 58.6 57.3 56.9 56.6 55.3 54.9 51.2 51.2 45.8 43.1 39.7 85.6 93.9 94.2 91.5 90.7 95.5 97.4 98.3 96.7 97.5 98.4 98.4 99.2 98.5 99.2 100.0 100.0 100.0 100.0 100.0 Overall success rate % Tundra vegetation shown as forest by the pollen (%) 79.1 84.6 83.5 82.4 80.2 82.4 85.7 87.9 81.3 85.7 80.2 81.3 79.1 79.1 79.1 47.4 85.7 73.3 68.8 61.1 93.8 84.6 100.0 94.1 92.3 100.0 94.1 100.0 100.0 94.7 α 2:1:0.5 with C & no √ α 3:1:0.5 with C & no √ Original PAIN √ C only with √ C only with no √ 71.4 71.4 70.3 65.9 57.1 100.0 100.0 100.0 100.0 100.0 Table S3 Comparison of the overall percentage correct match for pollen biomes versus vegetation for all Alaskan sites. Column 3 shows error due to tundra vegetation shown as forest by the pollen. The opposite error (forest shown as tundra by pollen) is 100 minus the value in column 3. Biomization method All Alaskan sites α 3:1:0.33 no √ α 2:1:0.5 √ α 3:1:0.5 √ Original PAIN √ α 4:1:0.25 no √ α 2:1:0.5 with C & √ α 3:1:0.5 with C & √ No Pinus or factors with √ α 4:1:0.25 with C & no √ α 3:1:0.33 with C & √ α 2:1:0.5 no √ α 3:1:0.5 no √ α 3:1:0.33 with C & no √ α 4:1:0.25 with C & √ C only with √ α 3:1:0.33 √ α 4:1:0.25 √ α 2:1:0.5 with C & no √ α 3:1:0.5 with C & no √ C only with no √ Overall success rate % Tundra vegetation shown as forest by the pollen (%) 79.4 79.1 79.1 78.8 78.8 78.8 78.8 78.8 78.5 78.5 78.2 78.2 76.9 76.6 76.3 75.3 73.7 73.7 73.7 66.5 55.4 40.9 40.9 74.6 34.3 71.6 71.6 74.6 76.5 52.9 75.4 75.4 83.6 37.8 93.3 24.4 16.9 92.8 92.8 98.1