CURRICULUM VITAE
NAME
Senior Research Associate
Kothapalli, Sesha Durga Kumar
Cornell University, Ithaca, NY-14853
Email: ksk25@cornell.edu
Weblink: http://www.human.cornell.edu/bio.cfm?netid=ksk25
EDUCATION/TRAINING (Beginning with bachelor’s degree.)
DEGREE
INSTITUTION AND LOCATION
(if applicable)
Andhra University
B.S.
Andhra University
M.S.
Osmania University
Ph.D.
Cornell University
Post doctorate
YEAR(s)
FIELD OF STUDY
1993
1995
2001
2002-2004
Botany, Zoology, Chemistry
CChemistry
Human Genetics
Human Genetics
Genetics & Genomics
Personal Statement
I have over 21 years of research experience and cross-training in Human Genetics, Genomics,
Pharmaco & Nutrigenomics. I started my research career, investigating Cytogenetic, Biochemical and Molecular
aspects of Human Genetic diseases and have published several research articles. My PhD work involved
investigating etiological factors responsible for recurrent pregnancy loss. The medical genetics experience
gained during my PhD program has helped me to perform cutting edge genetics and genomics work at Cornell
University.
As a postdoctoral fellow at Cornell University, I carried out genome-wide linkage studies in collaboration
with the Marshfield clinic and J. Craig Venter Institute. The comparative genomics experience gained in
postdoctoral training added a unique vantage to explore functional genomics studies.
In the last ten years as a research faculty member at Cornell, my work focused on genetic and molecular
aspects of long chain polyunsaturated fatty acids (LCPUFA) biosynthesis. LCPUFA are nutrients and bioactive
metabolites associated with most of the human diseases, specifically cardiovascular (CVD), neurological,
cancer, metabolic syndrome, and are also critical for growth and development. LCPUFA are precursors for
bioactive cell signaling eicosanoid and docosanoid molecules.
First, I was involved in studying global gene expression patterns in neonate primates supplemented with
LCPUFA. This work has resulted in identifying a biological network associated with nervous system
development.
We initiated a research thrust on characterization of molecular mechanisms controlling LCPUFA
biosynthesis, especially the fatty acid desaturases (FADS). Since 2009, we are publishing significant novel
findings:
1) an alternative biochemical pathway to the biosynthesis of eicosanoid precursors
2) discovery of multiple alternatively spliced isoforms of FADS genes
3) novel function of a splice variant of FADS1. This splice variant enhances FADS2 activity leading to
increased production of eicosanoid precursors.
4) conservation of alternative transcripts in several animal species.
5) unusual fatty acid synthesis in cancer cells and disturbance in eicosanoid signaling.
1
My personal interests: are in the areas of individualized medicine and nutrition based on pharmaco &
nutrigenetics and genomics approaches, human genetics, genetic counseling and molecular biology.
Accomplishments
It is important to note that I have an extensive research experience in Genetics, Genomics, Pharmaco &
Nutrigenomics, Molecular and Cell Biology of Lipids, conducted at many different institutions with an
interdisciplinary group of colleagues. In addition to research as a part of continuous medical education (CME)
program to Medical doctors, Human Genetics and Pharmacy students in India, I have given several talks about
diagnosing and managing inherited and acquired human genetic disease conditions (Medical Genetics). I am
also involved in promoting the growth of genetics education in high schools across the globe.
Positions and Employment
1995-1997: Junior Research Fellow, Institute of Genetics, Osmania University, India.
1997-2001: Senior Research Fellow, Institute of Genetics, Osmania University, India.
2002-2004: Postdoctoral Associate, Baker Institute, Cornell University, USA.
2004- Present: Research Associate/Senior Research Associate, Nutritional Sciences, Cornell University, USA.
Professional Memberships
2005-Present: American Society of Human Genetics
2005-2008: International Society of Neurochemistry
2006-Present: The International Society for the Study of Fatty Acids & Lipids
Work Experience:
Research Associate/Senior Research Associate, Cornell University, Dec. 2004 – Till Date
Key Responsibilities:
 Designed, performed and analyzed the gene expression microarray (DNA Chip) experiments (First
author on the manuscript and co-author on the Patent Invention Disclosures). Bristol-Myers Squibb
funded project.
 Created an assay by design Taqman probes (quantitative real time PCR) in association with ABI
Biosystems technical team for microarray (DNA Chip) data validation.
 Cloned and sequenced several partial and complete gene sequences. Sequences are deposited with
National Center for Biotechnology Information, USA.
 Effectively used several microarray (DNA Chip), next generation sequencing analysis software’s
including novel ingenuity pathway analysis software.
 Delivered talks on genetics and genomics research in various National and International
Conferences.
 Taught, trained and led several postdoctoral, graduate and undergraduate students in genomics and
molecular biology laboratory techniques.
 We identified a novel alternative biochemical pathway to the biosynthesis of eicosanoid precursors
involved in inflammation and several spliced transcript variants for fatty acid desaturases. Novel
findings. Published several articles on gene splicing.
 We have identified unusual fatty acid synthesis in cancer cells and disturbance of eicosanoid and
docosanoid signaling.
 Co-investigator: Functional characterization of desaturase genes (FADS1, FADS2 and FADS3).
 Co-investigator: Branched Chain Fatty Acids and Gut Development. (NIH funded project)
 Co-investigator: Docosahexaenoic acid supplementation and CNS function (Bristol-Myers Squibb ,
USA funded project).
 Co-PI: Long chain polyunsaturated fatty acid (LCPUFA) Status and Birth Outcome in India (NIHNICHD, USA funded project).
 Co-investigator: Genetic Regulation of Fatty Acid Biosynthesis. Supervised the work of several
employees for this project. Martek Biosciences/DSM, USA funded project.
2
 Co-investigator: Fatty Acid Bioequivalence. Supervised the work of several employees for this
project. Mead Johnson Nutrition, USA funded project
 Co-investigator:
Regulation of FADS gene expression by statin, LXR agonist GW3965,
rosiglitazone and hormone treatments.
 Collaborations with Cornell Scientists 1) Fatty acid analysis in FABP9 knockout mice 2) TSPO null
mouse study 3) RNA-sequencing of human milk fat globule transcriptome (Next Generation
Sequencing).
 Collaborated with Scientists at University of Buffalo, USA on FADS Proteomic project.
 Collaborating with Scientists at University of Cincinnati, USA on bipolar disease genetic studies.
 Collaborating with Scientists at Kaohsiung Medical University, Taiwan on diabetes genetic studies.
 Collaborating with Scientists at Massachusetts general Hospital on characterizing fatty acid profiles
of primary human fetal intestinal cells.
 Collaborating with Scientists at University of Kansas Medical Center on FADS2 INDEL genetic
studies.
 Collaborating with Scientists at University of Pune, India on FADS2 INDEL genetic studies.
 Collaborating with Scientists at University of Texas Medical Branch on characterizing adipose
tissue fatty acid profiles.
 Collaborating with Scientists at Gangneung-Wonju National University, Korea on characterization of
FADS alternative transcripts.
 Collaborating with Scientists at Jilin University, China on studying efficacy of dietary DHA using
animal models.
 Collaborating with medical doctor(s) at Arnot Health, Elmira, NY on studying fatty acid profiles of
tumor tissues.
 Collaborating with medical doctors at Arnot Health, Elmira, NY and Hyderabad India on studying the
effects of coconut oil nutrition and lipid parameters.
 Collaborating with medical doctor(s) at Cayuga Medical Center, Ithaca, NY on studying fatty acid
profiles of tumor tissues.
 Co-investigator: Efficacy of dietary docosahexaenoic acid (DHA) as a triglyceride or
phospholipids.
 Co-investigator: Fatty acid profiling of brown and white adipose tissue.
 Co-investigator: Fatty acid profiling of meconium samples from several animal species.
 Co-PI: Molecular mechanism of omega-3 response. (NIH-NCCIH, USA funded project)
Postdoctoral Research Associate, Cornell University (Jan.2002 to Nov. 2004)
Key Responsibilities:
 Performed genome-wide DNA marker association study in collaboration with Marshfield clinic to
identify candidate gene responsible for canine Sry-negative sex reversal syndrome.
 Designed, developed, optimized and validated several genetic markers individually and in
collaboration with J. Craig Ventor Institute for fine mapping project.
 Performed genotyping using high throughput screening capillary electrophoresis techniques.
 Carried out DNA purifications from various animal tissues, blotting techniques, whole mount in situ
hybridization, BAC library screening.
 Presented results at project team and departmental meetings.
 Created a canine pedigree for publication purpose.
 By applying modern molecular techniques we have excluded several candidate genes such as
PISRT1, WT1, DMRT1, GATA4, FOG2, SOX9, SF1, LHX1 and LHX9 that are involved in gonadal
differentiation and are thought to be responsible for canine Sry-negative sex reversal syndrome.
 A genome-wide linkage screen revealed highest LOD score of 3.4 on CFA29. Fine mapping
narrowed down the locus to a 5.4 Mb candidate region.
Junior and Senior Research Fellow, Institute of Genetics, Osmania University (Aug. 1995-Sep. 2001)
3
Key Responsibilities:
 Carried out human chromosomal analysis using various banding techniques and Fluorescence in situ
hybridization (FISH).
 Optimized and validated an ELISA assay to identify anticardiolipin autoimmune antibodies.
 Designed and conducted experiments to identify point mutation 677 C-T in MTHFR gene in women
experiencing recurrent pregnancy loss.
 HPLC analysis to identify plasma homocysteine levels.
 Designed and optimized spectrofluorometric assay for red cell selenium estimation.
 Animal cell culture (lymphocyte, chorionic villi, amniocyte and fibroblast cultures). DNA purifications
from human lymphocytes.
 Performed human cytogenetic, biochemical and molecular studies involving Down syndrome,
mental retardation, reproductive disorders, leukemia and sexual ambiguity (resulted in series of
publications).
 Taught and trained postgraduate medical doctors, postgraduate genetics and nursing students on
identification of Human Genetic disorders involving cytogenetic, biochemical and molecular genetic
tools.
Seminars Conducted:
 Took active part in conducting seminars for MS students, Andhra University, India (1993-1995).
Awards
1. PhD Research Fellowship by University Grants Commission (UGC). Exam score 91.66 percentile
(IIT, Kharagpur, India) being among the first 10% in the country.
2. Summer Institute in Statistical Genetics, course award to attend Advanced Pedigree MCMC module,
NC State University, Raleigh, NC, USA, 2004.
3. Summer Institute in Statistical Genetics, course award to attend Microarray Analysis module, NC
State University, Raleigh, NC, USA, 2005.
4. Travel grant award offered by Center for Vertebrate Genomics (VERGE), Cornell University, to attend
ISSFAL conference, Cairns, Australia, 2006.
5. Young investigator award granted by ISSFAL conference committee, Cairns, Australia, 2006.
6. Travel grant award offered by Center for Vertebrate Genomics (VERGE), Cornell University, to attend
European Society of Human Genetics Conference, Nice, France, 2007.
7. A seed grant from the Cornell Vertebrate Genomics Program to investigate "Functional Characterization
of FADS AT (Alternative Transcripts) in Long Chain Polyunsaturated Fatty Acid Biosynthesis."
8. Travel grant award offered by Center for Vertebrate Genomics (VERGE), Cornell University, to attend
International Congress of Human Genetics Conference, Montreal, Canada, 2011.
9. Subject of biographical record in 2012 Edition of Who's Who in America (Marquis Who’s Who).
10. Served as ad-hoc judge for Annual DNA Day Essay Contest (2015), conducted by The American
Society of Human Genetics (ASHG).
Pending Patent Applications
1. J. Thomas Brenna, K.S.D. Kothapalli, Joshua Anthony, Steven Rumsey, Zeina Jouni. New composition
and method useful for beneficiary global regulation of gene expression across diverse biological processes
including but not limited to regulation of lipid metabolism in infants and children
2. J. Thomas Brenna, K.S.D. Kothapalli, Joshua Anthony, Steven Rumsey, Zeina Jouni. New composition
and method useful for induction of expression of surfactant, pulmonary-associated protein B (SFTPB) and
other homologs in infants, children, and animals
3. J. Thomas Brenna, Holly T Reardon, K.S.D. Kothapalli. “FADS Regulation”
4
Cornell University Commercialization: Biomarker Discovery
1.Treatment of Cardiovascular Disease by Personalized Medicine. J.T. Brenna, H.T. Reardon, K.S.D.
Kothapalli
Review Activities
1. European Journal of Obstetrics & Gynecology and Reproductive Biology
2. Metabolism
3. Genetic Testing and Molecular Biomarkers
4. European Journal of Human Genetics
5. Journal of Lipid Research
6. British Journal of Nutrition
7. Gene
8. PLoS ONE
Consulting Activity
2011 Turtle Mountain, LLC, Oregon, USA
Research Publications
I have published under two last names (Kumar; Kothapalli); underlined below. *Co-corresponding Author,
† Equal Contribution.
1. H.G. Park, K.S.D. Kothapalli*, W.J. Park WJ, C. DeAllie, L. Liu, A. Liang, P. Lawrence, J.T. Brenna.
Palmitic acid (16:0) competes with omega-6 linoleic and omega-3 ɑ-linolenic acids for FADS2 mediated
Δ6-desaturation. Biochim Biophys Acta. 2016 Feb;1861(2):91-7.
2. J.Y. Zhang, K.S.D. Kothapalli and J.T. Brenna. Desaturase and elongase limiting endogenous long
chain polyunsaturated fatty acid biosynthesis. Current Opinion in Clinical Nutrition and Metabolic Care.
In Press (Invited Review Article).
3. X. Qin, H.G. Park, J.Y. Zhang, P. Lawrence, G. Liu, N. Subramanian, K.S.D. Kothapalli*, J. Thomas
Brenna. Brown but not white adipose cells synthesize omega-3 docosahexaenoic acid in culture.
Prostaglandins Leukot Essent Fatty Acids. 2016 Jan;104:19-24.
4. H. G. Park, W. J. Park, K. S. Kothapalli* and J.T. Brenna. The fatty acid desaturase 2 (FADS2) gene
product catalyze delta 4 desaturation to yield n-3 docosahexaenoic acid and n-6 docosapentaenoic acid
in human cells. FASEB J 2015; 29(9):3911-9.
5. V. Wijendran, Brenna JT, Wang DH, Zhu W, Meng D, Ganguli K, Kothapalli KS, Requena P, Innis S,
Walker WA. Long chain poly-unsaturated fatty acids attenuate the IL-1β-induced proinflammatory
response in human fetal intestinal epithelial cells. doi: 10.1038/pr.2015.154.
6. J. T. Brenna and K.S.D. Kothapalli. Commentary on Influence of virgin coconut oil enriched diet on the
transcriptional regulation of fatty acid synthesis and oxidation in rats–A comparative study. British Journal
of Nutrition. Br J Nutr. 2014 Sep 12:1-2.
7. L. Liu, N. Bartke, H.V. Daele, P. Lawrence, X. Qin, H.G. Park, K.S.D. Kothapalli, A. Windust, J. Bindels,
Z. Wang, J.T. Brenna. Higher efficacy of dietary DHA provided as a phospholipid than as a
triglyceride for brain DHA accretion in neonatal piglets. J Lipid Res. 2014 Mar;55(3):531-9.
8. V. Wijendran, I. Downs, C.T. Srigley, K.S. D. Kothapalli, W.J. Park, B.S. Blank, J.P. Zimmer, C.M. Butt,
Jr N. Salem, J.T. Brenna. Dietary arachidonic acid and docosahexaenoic acid regulate liver fatty acid
desaturase (FADS) alternative transcript expression in suckling piglets. Prostaglandins Leukot Essent
Fatty Acids 2013; 89(5):345-350.
9. H.T. Reardon, A.T. Hsieh, W.J. Park, K.S.D. Kothapalli, J.C. Anthony, P.W. Nathanielsz and J. T.
Brenna. Dietary long-chain polyunsaturated fatty acids upregulate expression of FADS3 transcripts.
Prostaglandins Leukot Essent Fatty Acids 2013; 88(1):15-19.
5
10. Woo Jung Park, K.S.D. Kothapalli*, J. Thomas Brenna et al. A novel fatty acid desaturase 1 isoform
potentiates FADS2-mediated production of eicosanoid precursor polyunsaturated fatty acids. J Lipid Res.
2012; 53(8):1502-12.
11. H.T. Reardon, J. Zhang, K.S.D. Kothapalli*, A.J. Kim, W.J. Park, and J. T. Brenna. Insertion-Deletions
In a FADS2 Intron 1 Conserved Regulatory Locus Control Expression Of Fatty Acid Desaturases 1 and 2
And Modulate Response To Simvastatin. Prostaglandins Leukot Essent Fatty Acids 2012; 87(1):25-33.
12. C. Tyburczy, K.S.D. Kothapalli, W.J. Park, B.S. Blank, Y. Liu, J.M. Nauroth, J. P. Zimmer, N. Salem Jr.,
J. T. Brenna. Growth, clinical chemistry and immune function in domestic piglets fed varying ratios of
arachidonic acid (ARA) and DHA. Br J Nutr 2012;107(6):809-816.
13. Woo Jung Park, K. S. D. Kothapalli*, Peter Lawrence, J. Thomas Brenna. FADS2 Function Loss at the
Cancer Hotspot 11q13 Locus Diverts Lipid Signaling Precursor Synthesis to Unusual Eicosanoid Fatty
Acids. PLoS ONE 2011, 6 (11): e28186.
14. H.T. Reardon, W.J. Park, J. Zhang, P. Lawrence, K.S.D. Kothapalli, and J. T. Brenna. PTB/hnRNP I
regulates fatty acid desaturase alternative splicing and cellular omega-3 fatty acid composition. J Lipid
Res 2011; 52(12):2279-86.
15. C. Tyburczy, K.S.D. Kothapalli, W.J. Park, B.S. Blank, K.L. Bradford, J. P. Zimmer, C.M. Butt, N. Salem
Jr., J. T. Brenna. Heart arachidonic acid is uniquely sensitive to dietary arachidonic acid and
docosahexaenoic acid content in domestic piglets. Prostaglandins Leukot Essent Fatty Acids 2011;
85(6):335-43.
16. C. Tyburczy, M.E. Brenna, J.A. Demari, K.S.D. Kothapalli, B.S. Blank, H. Valentine, S.P.
McDonough, D. Banavara, D.A. Diersen-Schade, J.T. Brenna. Evaluation of bioequivalency and
toxicological effects of three sources of arachidonic acid (ARA) in domestic piglets. Food Chem Toxicol
2011; 49(9):2320-7.
17. V. Selvaraj, A. Asano, J.L. Page, J.L. Nelson, K.S.D. Kothapalli, J.A. Foster, J.T. Brenna, R.S. Weiss,
A.J. Travis. Mice lacking FABP9/PERF15 develop sperm head abnormalities but are fertile. Dev Biol
2010; 348(2):177-189.
18. J.T. Brenna, K.S.D. Kothapalli, W.J. Park. Alternative transcripts of fatty acid desaturase (FADS) genes.
Prostaglandins Leukot Essent Fatty Acids, Review Article 2010, 82: 281-285.
19. W.J. Park, H.T. Reardon, C. Tyburczy, K.S.D. Kothapalli* and J.T. Brenna. Alternative splicing
generates a novel FADS2 alternative transcript in baboons. Mol Biol Reports 2010, 37: 2403-2406.
20. W.J. Park, K.S.D. Kothapalli †, H.T. Reardon, L.Y. Kim and J.T. Brenna. Novel fatty acid desaturase 3
(FADS3) transcripts generated by alternative splicing. Gene 2009, 446: 28-34.
21. W.J. Park, K.S.D. Kothapalli, P. Lawrence, C. Tyburczy and J.T. Brenna. An alternative pathway to long
chain polyunsaturates: The FADS2 gene product Delta8-desaturates 20:2n-6 and 20:3n-3. J Lipid Res
2009, 50: 1195-1202.
22. S.Pujar, K.S.D.Kothapalli, H.H.Goring, V.N. Meyers-Wallen. Linkage to CFA29 detected in a genomewide linkage screen of a canine pedigree segregating Sry-negative XX sex reversal. J Hered, 2007,
98:438-444.
23. K.S.D.Kothapalli, J.C.Anthony, B.S.Pan, A.T.Hsieh, P.W.Nathanielsz, J.T.Brenna. Differential cerebral
cortex transcriptomes of baboon neonates consuming moderate and high docosahexaenoic acid
formulas. PLoS ONE, 2007 Apr 11;2(4):e370.
24. K.S.D.Kothapalli, E.F.Kirkness, R.VanWormer, V.N.Meyers-Wallen. Exclusion of DMRT1 as a candidate
gene for canine SRY-negative XX Sex Reversal. Vet Journal, 2006, 171: 559-61.
25. K.S.D.Kothapalli, E.F.Kirkness, S.Pujar, R.VanWormer, V.N.Meyers-Wallen. Exclusion of candidate
genes for canine SRY-negative XX Sex Reversal. Journal of Heredity, 2005, 96:759-763.
6
26. S.Pujar, K.S.D.Kothapalli, E.F.Kirkness, R.H. Van Wormer, V.N.Meyers-Wallen. Exclusion of LHX9 as a
candidate gene for canine SRY-negative XX Sex Reversal in the American cocker spaniel model. Journal
of Heredity, 2005, 96:452-454.
27. K.S.D.Kothapalli, E.F.Kirkness, S.Pujar, V.N.Meyers-Wallen. Exclusion of WT1 as a candidate gene for
canine SRY-negative XX Sex Reversal. Animal Genetics, 2004, 35: 466-467.
28. K.S.D.Kothapalli, E.Kirkness, L.J.Natale, V.N.Meyers-Wallen. Exclusion of PISRT1 as a candidate locus
for canine SRY-negative XX sex reversal. Animal Genet, 2003, 34:467-469.
29. K.S.D.Kumar*, V.Govindaiah, S.E.Naushad, R.R.Devi, A.Jyothy. Plasma homocysteine levels correlated
to interactions between folate status and methylene tetrahydrofolate reductase gene mutation in women
with unexplained recurrent pregnancy loss. J Obstet Gynaecol, 2003, 23:55-8.
30. A.Jyothy, K.S.D.Kumar, G.N.Mallikarjuna Rao, M.Rajasekhar, B.Uma Devi, M.Sujatha, C.K.Kumari and
P.P.Reddy. Cytogenetic investigations in 1843 referral cases of disordered sexual development from
Andhra Pradesh, India. Int J Hum Genet India, 2002, 2: 55-59.
31. K.S.D.Kumar, M. Shiva Prakash and A.Jyothy. Beta2-glycoprotein I dependent anticardiolipin antibodies
in women experiencing recurrent pregnancy loss. Int J Hum Genet India, 2002, 2: 65-67.
32. K.S.D.Kumar*, A.Jyothy, M.Shiva Prakash, H.S.Rani, P.P.Reddy. Beta2-glycoprotein I dependent
anticardiolipin antibodies and lupus anticoagulant in patients with recurrent pregnancy loss. J Postgrad
Med, 2002, 48: 5-10.
33. K.S.D.Kumar*, Abhay Kumar, M.Shiva Prakash, V.Jagadeesan, A.Jyothy. Role of red cell selenium in
recurrent pregnancy loss. Journal of Obstetrics and Gynaecology, 2002, 22: 181-183.
34. A.Jyothy, G.N.Mallikarjuna Rao, K.S.D.Kumar, V.Babu Rao, B.Uma Devi, and P.P.Reddy. Translocation
Down syndrome. Indian J Med Sci, 2002, 56: 122-126.
35. V.Durga Rao, K.S.D.Kumar, V.Sridevi, M.Hema Prasad and P.P.Reddy. Adenosine Deaminase
estimation in petrol filling station workers. J Human Ecology, 2002, 13: 275-277.
36. A.Jyothy, K.S.D.Kumar, G.N.Mallikarjuna Rao, M.Rajasekhar, S.Ramesh Babu, C.Kusuma Kumari,
M.Sujatha and P.P.Reddy. Chromosomal role in the aetiology of amenorrhoea, sterility and reproductive
failure. J Obstet & Gynecol India, 2001, 51: 129-132.
37. B.Uma Devi, K.S.D.Kumar, S.Ramesh Babu, M.Swarna, P.P.Reddy. Serum adenosine deaminase in
tobacco factory workers. J Human Ecology, 2001, 12: 323-325.
38. A.Jyothy, K.S.D.Kumar, G.N.Mallikarjuna Rao, V.Babu Rao, B.Uma Devi, M.Sujatha, P.P.Reddy.
Parental age and the origin of extra chromosome 21 in Down syndrome. Journal of Human
Genetics, 2001, 46: 347-350.
39. A.Jyothy, K.S.D.Kumar, G.N.Mallikarjuna Rao, V.Babu Rao , M.Swarna , B.Uma Devi, M.Sujatha,
C.Kusuma Kumari, P.P.Reddy. Cytogenetic studies of 1001 Down syndrome cases from Andhra
Pradesh, India. Indian J Med Res, 2000, 111: 133-137.
40. M.Rajasekhar, K.S.D.Kumar, M.D.Sadhnani, A.Jyothy. Sexual ambiguity: Clinical and Cytogenetic
evaluation of 105 cases. Medical Science Research, 1999, 27: 205-207.
41. K.S. D.Kumar, G.N.Mallikarjuna Rao, C.Kusuma Kumari, A.Jyothy. Etiology of recurrent miscarriage: A
review. Int J Gynecol & Obstet India, 1999, 2: 25-34.
Selected Presentations
Oral and Poster Presentations
7
42. J.Y. Zhang, E. Kim, P. Lawrence, K.S. Kothapalli and J. Brenna. High Oleic Sunflower Oil Alters the
Proportions of n-6 and n-3 Polyunsaturated Fatty Acids in Young and Aging C57BL/6 Heart Tissue. The
FASEB Journal, vol. 29 no. 1 Supplement: 598.11.
43. H.G. Park, K. Kothapalli, W.J. Park, P. Lawrence, L. Liu and J.T. Brenna. Delta-6 Desaturase Substrate
Competition between 16:0 and 18:2n-6 or 18:3n-3 May Limit the Accumulation of 16:1n-10. The FASEB
Journal, vol. 29 no. 1 Supplement: 743.8.
44. J.Y. Zhang, X. Qin, H.G. Park, E. Kim, G. Liu, K.S. Kothapalli and J.T. Brenna. Alternative Splicing
Generates Novel Fads3 Transcript in Mice. The FASEB Journal, vol. 29 no. 1 Supplement: 743.10.
45. K.S.D. Kothapalli, X. Guo, X. Sun, S.S. Hyon, R. Ran-Ressler, J.S. Zou, Z. Gu and J.T. Brenna.
Alternative transcripts in the human milk fat globule proteinogenic RNA transcriptome and a novel
FADS2 transcript. International Society for the Study of Fatty acids & Lipids. 2014, Stockholm, Sweden.
46. K.S. Kothapalli, H.T. Reardon, and J.T. Brenna. Biomarker for Treatment to Lower Cholesterol by
Personalized Medicine. Ithaca, New York, USA.
47. K.S. Kothapalli, S.E. Carlson, K.O. O’Brien, K.E. Ojukwu, J.S. Zou, S.S. Hyon, R. Ran-Ressler and J.T.
Brenna. Allele frequency of a 22-bp insertion/deletion polymorphism of FADS2 in a U.S. population.
Experimental Biology. 2014, April, San Diego, USA.
48. K.S.D. Kothapalli, X. Guo, X. Sun, S.S. Hyon, R. Ran-Ressler, J.S. Zou, Z. Gu and J.T. Brenna.
Alternative transcripts in the human milk fat globule proteinogenic RNA transcriptome with emphasis on
desaturases. Experimental Biology. 2014, April, San Diego, USA.
49. J. Zhang, X. Qin, A. Liang, E. Kim, P. Lawrence, W.J. Park, K.S.D. Kothapalli, J.T. Brenna. Fatty acid
desaturase 3 (Fads3) null mouse biochemical phenotype. Experimental Biology. 2014, April, San Diego,
USA.
50. X. Qin, H.G. Park, J. Zhang, P. Lawrence, G. Liu, K.S. Kothapalli and J.T. Brenna. Fatty acid profiles of
undifferentiated and differentiated white and brown adipose cell lines supplemented with alpha-linolenic
acid. Experimental Biology. 2014, April, San Diego, USA.
51. H.G. Park, K.S. Kothapalli, W.J. Park, X. Qin, P. Lawrence and J.T. Brenna. Human breast cancer cells
stably expressed FADS2 synthesize sapienic acid (16:1n-10) from palmitic acid (16:0). Experimental
Biology. 2014, April, San Diego, USA.
52. L. Liu, N. Bartke, H. Van Daele, P. Lawrence, X. Qin, H.G. Park, K.S. Kothapalli, A. Windust, Z. Wang
and J.T. Brenna. Brain docosahexaenoic acid accretion is greater when supplied as phosphatidylcholine
than as triacylglycerol in piglets Experimental Biology. 2014, April, San Diego, USA.
53. K.S.D. Kothapalli, J.S. Zou, S. S. Hyon, K. E. Ojukwu, G. L. Bugbee, R. Alluri, H.K. Park, J. Zhang, R.
R. Ran-Ressler, and J. Thomas Brenna. Human Milk Fat Globule Proteinogenic mRNA Reveal Existence
of Alternative Transcripts. Experimental Biology. 2013, April, Boston, USA.
54. Kothapalli KSD. NS3320 Invited lecture on “Genes in Health and Disease”, October 2012, Cornell
University, USA.
55. Kothapalli KSD, Park WJ, Zhang J, Reardon HT, Zhang J, Downs I, Kaiser D, Hyon S, Brenna JT. An
Intron 1 Insertion/Deletion (Indel) Polymorphism In The Fatty Acid Desaturase 2 (FADS2) Gene Is
Associated With HUFA Synthesis In Cancer Cell Lines. International Society for the Study of Fatty acids
& Lipids. 2012, May, Vancouver, Canada.
56. Park WJ, Kothapalli KSD, Reardon HT, Lawrence P, Qian SB, Brenna JT. A novel fatty acid desaturase
1 (FADS1) isoform potentiates FADS2-mediated production of eicosanoid precursor polyunsaturated
fatty acids. International Society for the Study of Fatty acids & Lipids. 2012, May, Vancouver, Canada.
57. Reardon
HT,
Zhang
J,
Kothapalli
KSD,
Kim
AJ,
Park
WJ,
Brenna
JT.
Identification Of An Insertion-Deletion In FADS2 Intron 1 With SREBP Binding That Upregulates FADS1
And Mediates Enhanced HUFA Biosynthesis In Response To Simvastatin And LXR Agonists.
International Society for the Study of Fatty acids & Lipids. 2012, May, Vancouver, Canada.
8
58. Park WJ, Kothapalli KSD, Lawrence P, Brenna JT. Delta 4- desaturation activity coded by FADS1 is the
last step of 22:5 n-6 biosynthesis in human cells. International Society for the Study of Fatty acids &
Lipids. 2012, May, Vancouver, Canada.
59. Huang P, Jhang H, Hsu C, Kothapalli KSD, Brenna JT, Huang M. Polymorphisms in FADS2 correlated
with altered desaturase activity in a type-2 diabetic cohort. Experimental Biology. 2012, April, San Diego,
USA.
60. Kothapalli KSD, Park WJ, Lawrence P, Brenna JT, “Loss of FADS2 function at 11q13 cancer hot spot
region cause synthesis of unusual butylene-interrupted fatty acid.” the 12th International Congress of
Human Genetics/ASHG 61st Annual Meeting. 2011, October, Montreal, Canada.
61. Park WJ, Kothapalli KSD, Reardon HT, Lawrence P, Brenna JT. Identification of Novel Fatty Acid
Desaturase 1 Isoforms: Localization and Differential Expression in Tissues and Mammalian Cells. the
12th International Congress of Human Genetics/ASHG 61st Annual Meeting. 2011, October, Montreal,
Canada.
62. Park WJ, Kothapalli KSD, Tyburczy CT, Lawrence P, Brenna JT, “Novel alternative pathway for the
synthesis of LCPUFA.” Korean Society of Food Science and Technology. 2011, June, South Korea.
63. Reardon HT, Kothapalli KSD, Park WJ, Zhang J, Lawrence P, Brenna JT, “Alternative splicing of fatty
acid desaturases is associated with fatty acid composition.” Experimental Biology, Washington DC, USA,
2011, April.
64. Park WJ, Kothapalli KSD, Brenna JT. Novel FADS1 transcripts generated by alternative
polyadenylation, alternative transcription initiation and alternative splicing. The American Society of
Human Genetics. 2010, November, Washington DC, USA.
65. Brenna JT, Park WJ, Kothapalli KSD, Reardon HT. Fatty Acid desaturase alternative transcripts are
widely expressed and localize to mitochondria. International Society for the Study of Fatty acids & Lipids.
2010, June, Maastricht, The Netherlands.
66. Tyburczy CT, Kothapalli KSD, Park WJ, Blank BS, Salem Jr. N, Zimmer JP, Brenna JT. Effect of
arachidonic acid(ARA) levels on growth and clinical chemistry in domestic piglets. International Society
for the Study of Fatty acids & Lipids. 2010, June, Maastricht, The Netherlands.
67. Reardon HT, Park WJ, Kothapalli KSD, Brenna JT. Dietary LCPUFA upregulate FADS3 alternative
transcripts in neonatal baboon liver. Experimental Biology. 2010, April, Anaheim, USA.
68. Ran-Ressler RR, Kothapalli KSD, Glahn RP, Brenna JT. Cultured human intestinal cells take up and
metabolize branched chain fatty acids. International Society for the Study of Fatty acids & Lipids.
Maastricht, The Netherlands, 2010, June.
69. Ran-Ressler RR, Kothapalli KSD, Glahn RP, Brenna JT. Branched Chain Fatty Acids are taken up and
metabolized by Cultured Human Intestinal Cells (Caco-2). Experimental Biology. 2010, April, Anaheim,
USA.
70. Humans require omega-3 docosahexaenoic acid to achieve optimal brain and retinal fatty acid
composition. Proceedings of The International Seafood and Health Conference & Exhibition, Melbourne,
Australia. 2010.
71. Park WJ, Kothapalli KSD, Reardon HT, Brenna JT. Novel FADS3 alternative transcripts generated by
alternative splicing. The 5th Vertebrate Center for Genomics Annual Symposium in Cornell University,
2009, July, Ithaca, NY, USA.
72. Selvaraj V, Asano A, Page JL, Nelson JL, Kothapalli KSD, Foster JA, Brenna JT, Weiss RS, Travis AJ.
Mice lacking FABP9/PERF15 develop sperm head abnormalities but are fertile. The Society for the
Study of Reproduction’s annual meeting, 2009, July, Pittsburgh, USA.
73. Park WJ, Kothapalli KSD, Reardon HT, Brenna JT. Novel FADS3 alternative transcripts generated by
alternative splicing. Experimental Biology. 2009, April, New Orleans, USA.
9
74. Kothapalli KSD. "A partial reexamination of primate polyunsaturated fatty acid biosynthesis by cloning
and characterization of fatty acid desaturase (FADS) genes." VERGE Cornell University, Ithaca, NY,
2008, November.
75. Kothapalli KSD. Effects of high DHA formula on baboon neonates. Liver and brain gene expression.
Mead Johnson Nutritional, Evansville, Indiana, USA, 2008.
76. Park WJ, Kothapalli KSD, Lawrence P, Brenna JT, “A novel pathway for LCPUFA biosynthesis: FADS2
PRODUCT Δ8-DESATURATES 20:2n-6 TO 20:3n-6 AND 20:3n-3 TO 20:4n-3.” The 4th Vertebrate
Center for Genomics Annual Symposium in Cornell University, 2008, July, Ithaca, NY, USA.
77. Park WJ, Kothapalli KSD, Lawrence P, Brenna JT, “A novel pathway for LCPUFA biosynthesis: FADS2
PRODUCT Δ8-DESATURATES 20:2n-6 TO 20:3n-6 AND 20:3n-3 TO 20:4n-3.” International Society for
the Study of Fatty acids & Lipids. 2008, May, Kansas City, USA.
78. Kothapalli KSD, J.C. Anthony, B.S. Pan, A.T. Hsieh, P.W. Nathanielsz and J.T. Brenna. Impact of
Arachidonic acid and moderate and high Docosahexaenoic acid formulas on hepatic gene expression
profiles in baboon neonates. European Society of Human Genetics, 2007, Nice, France.
79. Kothapalli KSD, B.S. Pan, A.T. Hsieh, J.C. Anthony, P.W. Nathanielsz and J.T. Brenna. Comprehensive
Differential Transcriptome Analysis of Cerebral Cortex of Baboon Neonates Consuming Arachidonic Acid
and Moderate and High Docosahexaenoic Acid Formulas. Experimental Biology, 2006, San Francisco,
CA, USA.
80. Kothapalli KSD. Young investigator award lecture entitled “Differential transcriptomes of Liver and
Cerebral Cortex of baboon neonates consuming medium and high DHA.” ISSFAL, Cairns, Australia,
2006.
81. Kothapalli KSD, Kirkness E, Pujar S, Van Wormer R, Meyers-Wallen VN. Exclusion of candidate genes
for canine Sry-negative XX sex reversal, 2nd International Conference on Advances in Canine and Feline
Genomics, 2004, Universities Utrecht, Utrecht, The Netherlands.
82. Kothapalli KSD, Jyothy A. Role of Beta2-glycoprotein -I dependent anticardiolipin antibodies in women
experiencing recurrent pregnancy loss. 25th – Annual Conference, Indian Society of Human Genetics
and 2nd – Conference, South Asia-Pacific Society of Human Genetics, Nagpur, India, 2000.
Book Chapters
Recent Advances In Genetics & Health. V. Durga Rao, K.S.D. Kumar, M. Hemaprasad. Immunological
studies in petrol filling station workers. In: P.Usha Rani, A.Jyothy, M.Hema Prasad (eds) pp.203-206, 2004.
Paper Submitted
 K. S. D. Kothapalli, M.S. Gadgil, S.E. Carlson, K.O. O’Brien, J.Y. Zhang, H.G. Park, K. Ojukwu, J.
Zou, S.S. Hyon, K.S. Joshi, J. Thomas Brenna. Differences in allele frequencies of FADS2
rs66698963 Insertion/Deletion (Indel) polymorphism. Implications for endogenous synthesis of long
chain polyunsaturated fatty acids. J Hum Genetics.
Papers Under Preparation
 Ran-Ressler RR, Kothapalli KSD, Sim D, Glahn RP, Brenna JT. Branched chain fatty acids (BCFA)
are metabolized by Caco-2 cells.
 K. S. D. Kothapalli, Xiaoxian Guo, Xuepeng Sun, Stephanie S. Hyon, Rinat R. Ran-Ressler, Jiyao
Zhang, Hui Gyu Park, James Zou, Zhenglong Gu, J. Thomas Brenna. Human Milk Fat Globule
Proteinogenic RNA Alternative Transcripts.
10