Seasonal Effects on Preferential Flow on Irish Grassland Soils Gaelene Kramers1,2, Karl Richards1 and Nicholas M. Holden2 1 Teagasc, Environment Research Centre, Johnstown castle, Wexford, Ireland 2 UCD School of Agriculture, Food Science and Veterinary Medicine, University College Dublin Earlsfort Terrace, Dublin 2, Ireland gkramers@johnstown.teagasc.ie, kichards@johnstown.teagasc.ie, Nick.Holden@ucd.ie Agricultural land in Ireland is dominated by permanent grassland (> 5 yrs grass growth) for both grazing and silage. An important factor influencing water infiltration into grassland soils is preferential flow through macropores. These can be biological macropores caused by soil fauna or the decay of plant roots, or structural macropores caused by wetting and drying processes, freeze and thawing cycles or subsurface erosion (Beven and Germann, 1982). Grassland in Ireland is usually ploughed and reseeded every 7-10 years, if used for intensive livestock production. As only 2% of grassland by area is reseeded each year, there is stable soil structure and biological activity, with rapid recovery after disturbance in a well managed grassland where grazing is prohibited in the winter months and other wet periods. The extent to which macropores occur in a soil under grassland will be influenced by land management, climate and soil texture. The Irish climate is characterized by mild temperatures with a wet winter and drier summer (Rohan, 1986). Grass growth and biological activity peak in the mild, wetter months of spring and slow down in the warmer, drier months in summer. Preferential flow in grassland soils is usually initiated either at the soil surface or in a near-saturated surface soil layer (Weiler and Naef, 2003). In Irish grassland soils the water content of the surface root zone will normally be greater in spring than in summer (Schulte et al., 2005). The seasonal difference in water content, evaporation, temperature and biological activity in Irish grassland soils could have an impact on the extent of preferential flow occurring at different times of the year. This study was designed to ascertain whether there is seasonality associated with preferential flow in Irish grassland soils. Using blue food dye (brilliant blue FCF), infiltration patterns will be studied on three soil types in spring (March-April) and in summer (June-July). The three soils are a sandy loam, a loam, and a clay loam which are all under permanent grassland. A continuous rainfall intensity of 4 mm/hr will be applied for 8 hours with a rainfall simulator in summer and in spring. The soil onto which the dye is applied will be excavated and the infiltration patterns analysed using digital image analysis. The amount of large macropores contributing to soil water flow as well as the actual infiltration patterns will be used to determine whether there is any difference in the character of the infiltration between spring and summer. The soils will be tested for water repellence during both experiments. Meteorological data and soil temperature measurements will further enhance the dataset. Preliminary results will be presented and discussed. References Beven, K. and P. F. Germann (1982). "Macropores and Water Flow in Soils." Water Resources Research 18(5): 1311-1325. Rohan, P. K. (1986). The climate of Ireland. Dublin, Meteorological Service, Ireland. Schulte, R. P. O., J. Diamond, F. K., N. M. Holden and A. J. Brereton (2005). "Predicting the soil moisture conditions of Irish Grasslands." Irish Journal of Agricultural and Food Research 44(1): 95110. Weiler, M. and F. Naef (2003). "An experimental tracer study of the role of macropores in infiltration in grassland soils." Hydrological Processes 17: 477-493.