Sample Job Descriptions and Summaries For use in the Department of Microbiology Tech Positions: Responsibilities and Duties Order supplies Unpack and restock lab supplies to maintain lab supply inventory Prepare media and solutions Oversee growth and maintenance of cell lines Tissue culture: grow and maintain cells Perform Western and Southern blots PCR analysis Assist lab members with experiments Keep accurate records for radioactive supplies Check on liquid nitrogen freezer Autoclave pipette tips, microfuge tubes and surgical instruments Summary (example 1): Maintain cell lines and prepare virus for use by lab members; aid in animal work and participate in minor experiments in molecular biology and virology; order lab supplies; keep chemical inventories; organize freezers. Summary (example 2): Assist PI in planning protocols. Perform microbial and molecular biology techniques; perform DNA, RNA and protein analysis. Maintain log book and lab notebook; write lab reports. Assist in ordering lab supplies. Attend lab meetings. Qualifications for a “Research Technician C”: This is a weekly paid position with slightly less responsibilities than a Research Specialist. Usually this person would have a BS or BA in Chemistry or Biology, lab experience preferred but not required. The average starting salary would be between $23K-$25K. Research Specialists: Responsibilities and Duties: Design protocols Execute experiments Prepare media and solutions Perform cell culture Maintain and expand tissue culture and virus stocks Create virus stocks and perform assays Perform DNA preparations using Qiagen, Biorad and Promega kits Perform molecular biological assays Cloning and protein purification RNA isolation and purification Breed mouse strains, type mice Run mouse core: DNA analysis of transgenics, perform FACs analysis and interact with ULAR staff on regular basis Drug administration to mice Test entry inhibitors and antibodies in cell-cell fusion assays Assist senior postdocs with cornea scarifications Conduct cell culture HIV and SIV infection assays in a BSL-3 facility Present research data at lab meetings Supervise work-study students Liason with vendors representatives Data analysis Summary (example 1): Principal duties include experimental work, literature searches, presentations and general laboratory administration. Other specific aspects of the job involve analysis of cell adhesion, tissue culture, flow cytometry, immunofluorescence, protein analysis by gel electrophoresis and blotting, protein cross-linking and radio-iodination of protein for analysis of adsorption and specific binding, and ELISA analysis. Summary (example 2): Perform microbiological and immunolical experiments involving listeria monocytogenes in mice; breed and maintain mouse strains; create virus stocks; present data at lab meetings. Molecular biology techniques, biochemical assays and cell biological approaches will be pursued. Summary (example 3): Contribute to research projects in leading HSV lab. Responsible for managing the lab by directing students, ordering supplies, assisting with animal surgeries, etc. Qualifications for a Research Specialist: There are four levels of Research Specialist, though it is most common to hire a new person as a Research Specialist A and over years promote them through the ranks. Research Specialist A: BS or BA in chemistry or biology with some lab experience required. Common starting salary is $24K-$27K Research Specialist B: BS or BA required, MS preferred with at least 1-3 years lab experience required. Common starting salary is $27K-$32K. Research Specialist C: MS preferred and sometimes required with 3-5 years lab experience. Common starting salary ranges $32-50K. Research Specialist D: Please discuss with Micro Office staff before posting this position for an external hire.