De Lys E. Coli bacteria
L Broth
Ampicillin
Chloramphenicol
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Laura Machesky
Assessor
Jacqui Woodings
Assessment Number*
2031
Date of Assessment
15.01.02
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
List names where possible
J.A.Woodings, C. Costa, S. Launay, T. Millard, L.Machesky, S. Sharp
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
8th Floor labs
Production of recombinant proteins in bacteria
AMOUNT**
De Lys E. Coli bacteria
Attach copies of data sheet(s)
HAZARD
Biological
(Signalling to
Cell Motility)
Caution
Harmful
L Broth
Ampicillin
2L
2ml (1M)
Chloramphenicol
200l
(25mg/ml)
Toxic
IPTG
800l
(0.5M)
Harmful
PBS
Tris
200ml
500ml
(50mM)
Non Hazardous
Harmful
EDTA
200ml
(40mM)
Harmful
Sucrose
200ml
(2.5% w/v)
Non Hazardous
RISK PHRASES
See biohazard risk
assessment “Protein
Expression in E. coli”
Not Fully tested
May cause sensitisation
by inhalation and skin
contact. R42/43
May cause cancer. R45
May cause heritable
genetic defects. R46
Possible risk of harm to
the unborn child. R63
Harmful by inhalation, in
contact with skin and if
swallowed. R20/21/22
Irritating to eyes,
respiratory system and
skin. R36/37/38
None known
Harmful by inhalation, in
contact with skin and if
swallowed. R20/21/22
May cause sensitisation
by inhalation and skin
contact. R42/43
Harmful if swallowed.
R22
May be harmful if inhaled
or through skin contact.
Irritating to eyes,
respiratory system and
skin. R36/37/38
None known
HAZDAT NO***
BIOSCIENCESNO***
Sodium Azide
500ml
(0.02%
w/v)
Very Toxic
MgCl2
200ml
(100mM)
Irritant
Triton X-100
200ml
(0.2% w/v)
Harmful
Tween
100ml
(0.2%w/v)
200ml
(1mM)
Caution
Thrombin
30units
(1u/l)
Harmful
CaCl2
100ml
(2.5mM)
Harmful
PMSF
100l
(0.1M) in
ethanol
Very toxic
Ethanol
100l
GSH Agarose
Glutathione
5ml
25ml
(100mM)
100l
Highly
Flammable
Caution
Non Hazardous
DTT
p-amino benzamideagarose
5
Irritant
Non Hazardous
Very toxic if swallowed.
R28
Contact with acids
liberates very toxic gas.
R32
Heating may cause an
explosion. R5
Irritating to eyes,
respiratory system and
skin. R36/37/38
Harmful by inhalation, in
contact with skin and if
swallowed. R20/21/22
Risk of serious damage
to eyes. R41
Not Fully tested
Irritating to eyes,
respiratory system and
skin. R36/37/38
Harmful by inhalation, in
contact with skin and if
swallowed. R20/21/22
Harmful if swallowed.
R22
Irritating to eyes,
respiratory system and
skin. R36/37/38
Toxic by inhalation, in
contact with skin and if
swallowed. R23/24/25
Causes burns. R34
Contact with water
liberates highly
flammable gases. R15
Highly flammable. R11
Not Fully tested
None known
None known
INTENDED USE
Give brief details and attach protocol/instructions
To purify recombinant proteins from bacteria
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
There is a possible risk of harm to the unborn child when using Chloramphenicol. There is a risk involved when
making up the solutions from solid reagents. The risk is reduced considerably when handling the dilute solutions.
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The level of risk, when making up the solutions from solid reagents, is unacceptable. When handling the dilute
solutions, risks are greatly reduced provided good laboratory practice is followed.
8
CONTROL MEASURES
Additional to Good Chemical Practice
Weigh toxic chemicals inside a fume hood (7th floor). Alternatively, weigh toxic chemicals in a well-ventilated area
whilst wearing a facemask, taking care not to raise aerosols.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
Use of fume hood and balance
10
MONITORING
Performance of control measures,
Check fume hood and balance are in safe working order
Personal exposure
Health Surveillance
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Discard solutions down the sink with plenty of running water.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
January 2007
13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Dilute solids with plenty of water and mop up with absorbent paper. Discard paper in yellow clinical waste bags
for incineration.
If a major spill involves Chloramphenicol, IPTG, azide or PMSF solids, dispose of them using the special waste
procedures (contact Brian Facer in stores).
If a major ethanol spill occurs, remove any sources of heat or ignition.
Do not mix azide with acids.
TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid
The person cleaning up should wear lab coat, gloves, eye protection and a mask.
If chemicals come into contact with skin or eyes, wash with plenty of running water.
Remove pregnant women from the vicinity of any spill of chloramphenicol.
Clean-up/decontamination
TO RENDER SITE OF EMERGENCY SAFE
Dilute solids with plenty of water and mop up with absorbent paper. Discard paper in yellow clinical waste bags
for incineration
If a major spill involves Chloramphenicol, IPTG, azide or PMSF solids, dispose of them using the special waste
procedures (contact Brian Facer in stores).
CONTACT
Laura Machesky
PHONE
42504
*
Prefix T is used for Teaching Assessment Number.
**
List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =
4g).
***
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.