單 位:農學院獸醫學系 教師姓名:吳瑞得 現 職:助理教授 學 歷:國立中興大學獸醫學士 (1993 - 1998) 國立中興大學獸醫學博士 (1998 - 2006) Name:Jui-Te Wu E-mail:jtwu@mail.ncyu.edu.tw TEL :05-2717574 FAX :05-2717566 簡要經歷: 國立嘉義大學獸醫學系助理教授 (2007 - ) 考試: 考試院獸醫師檢覈考試及格 ( 2000 ) 專長: 動物疾病學、動物繁殖障礙與繁殖技術、精子生理功能研究、蛋白質純化鑑定與 應用 研究興趣: 精子生理功能研究、精液保存技術與人工輔助繁殖技術、鹿茸生理與生化研究 主要教授課程: 獸醫外科學及實習、臨床討論、診療實習、高等小動物外科學、專題討論 最近參與的研究計畫案: 計畫名稱 擔任工作 起訖年月 補助機構 豬精子性特異蛋白之特性研究與應用-抗體製備與功能定性研 兼任助理 95.08 – 96.07 國科會 X-及 Y-精子細胞膜特異性蛋白純化及應用 兼任助理 92.08 – 94.07 國科會 Applications of purified nongenomic sperm progesterone receptor 兼任助理 91.08 – 93.07 國科會 究 proteins (1/2) Production and functional characterization of monoclonal antibody against sperm nongenomic progesterone receptor(s) (2/2) Study of reproductive characteristics of animals immunized by purified sperm progesterone receptor proteins (A)期刊論文 1.JT Wu, KC Tung, HP Fung, TJ Guo, PS Tsai, FP Cheng. Evaluation of Reproductive Characteristics of farmed Formosan Sika deer (Cervus nippon taiouanus) and Formosan Sambar deer (Cervus unicolor) during breeding season: an electro-ejaculation study. Taiwan Veterinary Journal 2002; 28:204-210. 2.KC Tung, CH Lai, FP Cheng, JT Wu, CH Yang, JS Wang. Comparisons of larvae morphological properties of Setaria spp. in the cattle, goat and deer with cerebrospinal setariasis. Taiwan Veterinary Journal 2002; 28:220-227. 3.FP Cheng, TJ Guo, JT Wu, TE Lin, PJF Ursem, B Colenbrander and HP Fung. Annual variation in semen characteristics of pigeons (Columba livia). Poultry science 2002; 8:11050-11056. (SCI) 4.FP Cheng, YT Tsai, ID Tsai, JT Wu, HP Fung. Case report: Effects of electro-acupuncture on canine monoparesis caused by organphosphorus intoxication. Taiwan Veterinary Journal 2003; 29:151-155. 5.ID Tsai, KC Tung, CH Lai, JT Wu, CL, Hsu, JS Wang, FP Cheng. In vitro lysis of canine heartworms using bromelain. Taiwan Veterinary Journal 2003; 29:116-121. 6.ID Tsai, KC Tung, CH Lai, JT Wu, JS Wang, FP Cheng. Epidemiological survey of Dirofilariasis of stray dogs in Taichung area. Taiwan Veterinary Journal 2003; 29:71-75. 7.TJ Guo, JT Wu, JP Kao, CH Cheng, WC Cheng, JPW Chan, FP Cheng. A morphological study on racing pigeon Spermatozoa (Columa livia). Taiwan Veterinary Journal 2003; 29:333-339. 8.TJ Guo, JT Wu, JP Kao, JPW Chan, FP Cheng. Study of assisted artificial breeding in pigeons ( Columa livia). Taiwan Veterinary Journal 2003; 29:400-404. 9.KC Tung, FP Cheng, JT Wu, SC Wen, CM Weng, CH Yang, JS Wang. The epidemiological survey of genus Setaria in the farmed cattle and deer in Kinmen Area. Taiwan Veterinary Journal 2003; 29:373-377. 10.FP Cheng, JT Wu, HP FUNG, JS Wang, KC Tung. Effect of different extenders on post-thaw sperm survival, acrosomal integrity and longevity in cryopreserved semen of Formosan Sika deer and Formosan Sambar deer. Theriogenology 2004; 61:1605-1616. (SCI) 11.WC Cheng, MC Liau, JT Wu, CH Shyu, CH Cheng, KC Chiang, SH Chiou, JH Shien, CL Shyu, FP Cheng. Proliferation - inhibition of DMGT and LM tk cell lines by application of CDA-Ⅱ and high doses of Vitamin C and Vitamin K3. Taiwan Veterinary Journal 2005; 31:128-137. 12.FP Cheng, JT Wu, PS Tsai, CLT Chang, SL Lee. Effects of cryo-injury on progesterone receptor(s) of canine spermatozoa and its response to progesterone. Theriogenology 2005; 64:844-854. (SCI) 13.JT Wu, PS Tsai, SL Lin, FP Cheng. Characterisation of the progesterone receptor on canine spermatozoa. Reproduction, Fertility and Development 2005; 17:733-741. (SCI) 14.JT Wu, KC Chiang, FP Cheng. Expression of progesterone receptor(s) during capacitation and incidence of acrosome reaction induced by progesterone and zona proteins in boar spermatozoa. 2005 spring symposium. Animal Reproduction Science 2006; 93:34-45. (SCI) (B)研討會論文 Poster 1.FP Cheng, TJ kuo, JT Wu, TE Lin. 1999; December. Seminal characteristics of racing pigeons in Taiwan. The Taiwan Journal of Veterinary Medicine and Animal Husbandry 69; suppl 1:125 abs. 2.FP Cheng, JT Wu, KC Tung, TJ Kuo, HP Fung. 1999; December. Seminal characteristics of farmed Formosa Sika deers. The Taiwan Journal of Veterinary Medicine and Animal Husbandry 69; suppl 1:126 abs. 3.FP Cheng, JT Wu, KC Tung, HP Fung. 2000; May. Seminal characteristics and semen cryopreservation of farmed Formosa Sika deers and Formosan Sambar deer. The Taiwan Journal of Veterinary Medicine and Animal Husbandry. 4.FP Cheng, JT Wu, KC Tung, HP Fung. 2000; November. Semen cryopreservation of Formosan Sika deer and Formosan th Sambar deer. 11 congress of the Federation of Asian Veterinary Associations. P369. 5.FP Cheng, JT Wu, PS Tsai, SL Lee. 2000; December. The effect of freezing injury on progesterone receptor(s) of frozen canine spermatozoa. Journal of Reproduction and Fertility 26; 25-26 abs. 6.WW Chen, FP Cheng, ID Tsai, JT Wu, JS Wang. 2001. The effect of centrifugation and time on the pack red cells preparation. Spring Meeting of Association of Taiwan Husbandy and Veterinary Sciece. P67. 7.KC Tung, JT Wu, CH Lai, HK Ooi, JS Wang. 2001. Matrix metalloproteinases in the cerebrospinal fluid of goats with setariasis. Spring Meeting of Association of Taiwan Husbandy and Veterinary Sciece. P95. 8.JT Wu, KC Tung, HP Fung, JS Wang, FP Cheng. 2001. Effect of Different Extenders on post-thaw sperm survival, acrosomal integrity and longevity in cryopreserved semen of Formosan Sika deer and Formosan Sambar deer. Joint Meeting of Association of Taiwan Husbandry and Veterinary Science and Chinese Society of Veterinary Medicine. P104. 9.ID Tsai, KC Tung, CH Lai, JT Wu, CL Shyu, JS Wang, FP Cheng. 2002; May. The in vitro lysis of canine heartworms by the use of bromelain. Spring Meeting of Association of Taiwan Husbandy and Veterinary Sciece. P68. 10.KC Chiang, JT Wu, PS Tsai, HP Fung, FP Cheng. 2002; December. Investigation of porcine sperm progesterone receptor(s) exposure and progesterone-mediated acrosome reaction during in vitro capacitation. Joint Meeting of Association of Taiwan Husbandry and Veterinary Science and Chinese Society of Veterinary Medicine. P162. 11.KC Tung, CH Lai, FP Cheng, JT Wu, YT Chiu, CH Yang, SC Wen, HK Ooi, JS Wang. 2003; May. The biological study of Setaria nematodes. Spring Meeting of Association of Taiwan Husbandy and Veterinary Sciece. P76. 12.FP Cheng, JT Wu, JPW Chan, JS Wang, B Colenbrander, KC Tung. Effect of different extenders on post-thaw sperm survival, acrosomal integrity and longevity in cryopreserved semen of Formosan Sika and Formosan Sambar deer. Fertility 2003, Aberdeen UK. Reproduction abstract serial 30, p86-87. 13.FP Cheng, JT Wu, PS Tsai, CLT Chang, SL Lee. The fate of cryo-preserved progesterone receptor(s) of canine sperm and its subsequent response to ligand stimulation. Fertility 2003, Aberdeen UK. Reproduction abstract serial 30, p86. 14.JT Wu, PS Tsai, HP Fung, SL Lee, FP Cheng. Characterization and purification of sperm progesterone receptor(s). Fertility 2003, Aberdeen UK. Reproduction abstract serial 30, p87. 15.FP Cheng, KC Chiang, JT Wu. 2004; April. Expression of progesterone receptor(s) during capacitation and incidence of acrosome reaction induced by progesterone and zona proteins in boar spermatozoa. Joint European Conference in Reproduction and Fertility 31, p12. 16.WC Cheng, MC Liau, HC Wang, JT Wu, CH Cheng, KC Chiang, SH Chiou, JH Shieh, CL Shyu, FP Cheng. 2004; May. Proliferation inhibition of JKT-1, DMGT and LMtk- cell lines by CDA-II, vitamin C, and vitamin K3. Spring Meeting of Association of Taiwan Husbandy and Veterinary Sciece. P61. 17.KC Chiang, JT Wu, CH Cheng, WC Cheng, FP Cheng. 2004; May. Roles of progesterone in inducing the acrosome reaction of boar spermatozoa. Spring Meeting of Association of Taiwan Husbandy and Veterinary Sciece. P69. 18.CH Cheng, JT Wu, KC Chiang, WC Cheng, SH Chiou, JH Shien, CL Shyu, FP Cheng. 2004; May. Use of opposite-sex immunization to screen sex-specific proteins from X- and Y-sperm plasma membrane. Spring Meeting of Association of Taiwan Husbandy and Veterinary Sciece. P70. 19.JT Wu, YH Fan, SH Chiou, FP Cheng. 2005; May. Detection and characterization of progesterone receptor transcript in boar sperm. Spring Meeting of Association of Taiwan Husbandy and Veterinary Sciece. P105. 20.CY Lee, SY Huang, JT Wu, YC Shieh, FP Cheng. 2005; December. Detection of sex-specific proteins from porcine sperm membrane. Autumn Meeting of Association of Taiwan Husbandy and Veterinary Sciece. P116. Oral Presentation 1.JT Wu, PS Tsai, FP Cheng. 2002; May. Characterizations of non-genomic progesterone receptor on canine sperm. Spring Meeting of Association of Taiwan Husbandry and Veterinary Science. P31. 2.KC Chiang, JT Wu, CH Cheng, WC Cheng, FP Cheng. 2004; May. Roles of progesterone in inducing the acrosome reaction of boar spermatozoa. Spring Meeting of Association of Taiwan Husbandry and Veterinary Science. P70. 3.JT Wu, KC Chiang, FP Cheng. 2005; May. Capacitation enhances the A23187-induced calcium influx and the subsequent acrosome reaction in the boar sperm. Spring Meeting of Association of Taiwan Husbandry and Veterinary Science. P23. 4.JT Wu, KC Chiang, FP Cheng. 2005; May. Expression of progesterone receptor(s) during capacitation and incidence of acrosome reaction induced by progesterone and zona proteins in boar spermatozoa. Spring Meeting of Association of Taiwan Husbandry and Veterinary Science. P24. (C)其他著作 1.吳瑞得. 2000. 台灣畜養梅花鹿及水鹿之人工輔助繁殖。碩士班研究成果報告。國立中興大學獸醫學研究所。 台中。 2.吳瑞得. 2006. 哺乳動物精子助孕素接受器之特性研究。博士論文。國立中興大學獸醫學研究所。台中。