DNA Learning Center www.urbanbarcodeproject.or g Phone: (516) 367-5170 Fax: (516) 367-5182 Email: urbanbarcode@cshl.edu Cold Spring Harbor Laboratory 1 Bungtown Road Cold Spring Harbor, NY 11724 Urban Barcode Project Footlocker Kit Application Form Date Teacher Name Teacher Email Teacher Phone School Name School Address School Phone Grade of Students Teams using this kit Types of Samples and Number of DNA extractions (Please indicate the number of DNA extractions you plan to perform for each sample type) I would like this kit by... (choose days between Tuesday and Friday) Do you have any equipment/ materials for this lab? (Please enter X for answer) # teams: ________ # students (total): ______ Project titles: ____________________________________ ____________________________________ ____________________________________ ____________________________________ Plant Algae Fish Insect #______ Other animals (specify the type and #): #______ Type:_____________________ #_______ #______ #______ Date: Yes ________ No __________ If you have equipment/materials, please complete page 2. _________________________________________________________________________________________ FOR OFFICE USE ONLY Pick-up Date Return Date Fee DNA Learning Center Cold Spring Harbor Laboratory 1 Bungtown Road Cold Spring Harbor, NY 11724 www.urbanbarcodeproject.or g Phone: (516) 367-5170 Fax: (516) 367-5182 Email: urbanbarcode@cshl.edu If you have any of the following equipment/materials at your school, please indicate it below with a “Y”. Equipment/Materials DNA extraction reagents (silica extraction method) Pestles for grinding samples in microcentrifuge tubes Microcentrifuge Waterbath Thermometer Gel electrophoresis chambers / power supplies Gel electrophoresis reagents (agarose, buffer etc.) UV transilluminator Digital camera and hood for gels Thermal cycler (if “Y”, what model?) Tubes for thermal cycler Small micropipettors (10 µl) Medium micropipettors (100 µl) Large micropipettors (1000 µl) 10 µl tips 100 µl tips 1000 µl tips 1.5 ml microcentrifuge tubes Tube racks for 1.5ml tubes, PCR tubes, etc. DNA stain (e.g. SYBR green, CarolinaBLU, etc.) DNA marker (e.g. 100bp ladder, pBR322/BstNI) Sterile distilled water Gloves Enter “Y” below if you have any of these materials.