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Appendix S2. Hierarchical analysis of variance of distribution and climate variables for each of the disjunct
2
genera.
3
4
Orders and families of flowering plants follow the Angiosperm Phylogeny Group II (2003) classification
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incorporating recent information from molecular phylogenetic analyses (e.g., Soltis et al. 2000). We
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calculated a hierachical analysis of variance for 9 variables in each region (log10-transformed area [Area],
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mid-latitude [Midlat], northernmost latitude [Nmost], latitude range [Range], average annual temperature
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[AnnT], average January temperature [JanT], average July temperature [JulT], annual precipitation [AnnP],
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summer [June-September] precipitation [SumP]). Levels of analysis were orders within angiosperms,
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families within orders, and genera within families (error). Analyses were done with the Statistical Analysis
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System (SAS) NESTED procedure. A preliminary analysis included all taxa, but because of the unbalanced
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design the SAS software does not estimate statistical significance of the variance components. None of the
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order or family-within-order effects was significant (P < 0.05) when the analysis was run as a nested general
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linear model (SAS Proc GLM). The variance distributions from Proc NESTED are reported in the following
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table for eastern Asia and eastern North America separately.
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df
Area
Midlat
Nmost
Range
AnnT
JanT
JulT
AnnP
SumP
23
7.8
21.0
0.0
0.0
0.0
0.0
0.0
7.0
3.1
Families 17
0.3
0.0
50.8
36.9
24.5
22.6
20.4
0.0
0.0
Genera
91.9
79.0
49.2
63.1
75.5
77.4
79.6
93.0
96.9
Eastern Asia
Orders
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Eastern North America
Orders
17
23
0.3
39.1
17.6
17.1
15.0
16.7
14.1
15.5
20.5
Families 17
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
Genera
99.7
60.9
82.4
82.9
85.0
83.3
85.9
84.5
79.5
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In a second analysis, the data were randomly trimmed to produce a balanced design, with two genera in each
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of two families in each of six orders, for a total of 24 genera. In this reduced dataset, the SAS output includes
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estimates of the statistical significance of differences between families within orders, and orders within
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angiosperms.
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df
Area
Midlat
Nmost
Range
AnnT
JanT
JulT
AnnP
SumP
0.0
0.0
0.0
14.2
0.0
0.0
0.0
3.4
0.0
Families 6
32.4
46.4
68.0
5.5
46.6
46.1
40.0
0.0
0.0
Genera
67.6
53.6
32.0
80.3
53.4
53.9
60.0
96.6
100.0
Eastern Asia
Orders
5
12
Eastern North America
Orders
5
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
Families 6
0.0
46.4
0.0
29.7
3.0
10.2
14.8
0.0
0.0
Genera
100.0
53.6
100.0
70.3
97.0
89.8
85.2
100.0
100.0
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6
7
8
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Variance proportion in bold type is P < 0.01.