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Clinical Chemistry Instrumentation Study Sheet:
Spectrophotometer:
Components:
Light source:
Visible spectrum: Tungsten Lamp
UV spectrum: Mercury- Arc
Deuterium
Monochromators: Separate light (radiant energy) into wavelengths
Filters
Prisms
Diffraction gratings
Photodetectors (Transducers): Convert radiant energy to electrical energy
Photocell (barrier-layer cell)
Phototube
Photomultiplier Tube
Photodiode
Principle: Absorbance of Light is proportional to Concentration
Atomic Absorption Spectrophotometry:
Components:
Light source:
Hollow cathode Lamp: Iight emitted is single wavelength characteristic for metal being tested.
Chopper: alternates light signals directly from hollow cathode lamp and light emitted from
flame
Sample:
Atomized in flame
Principle:
Light absorbed in the flame is proportional to concentration of metal
Reflectance Photometry:
Components: Dry slide technology
Principle:
Light is absorbed by sample in the slide; Amount of reflected light is inversely proportional to
concentration.
Nephelometry:
Principle: Light scatter by particles; Light detector at 30-90 degree angle from beam
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