KYLE TRETINA 4 Wheaton Center Apt. 401, Wheaton, Il 60187 Email: kyletretina@gmail.com Phone: 856-630-1025 OBJECTIVE To begin study in an applied research microbiology and immunology graduate degree program that will further enhance my analytical skills, research experience, and educational success to prepare me for a career in medical research EDUCATION Bachelor of Science, Interdisciplinary Studies – Biotechnology May 2011 Wheaton College, Wheaton, Illinois GPA: 3.52/4.0 Related Coursework: Microbiology & Immunology, Genetics, Bioinformatics, Organic Chemistry I&II, Biochemistry, General Physics I&II, Calculus I&II RESEARCH Research Technologist June 2011 – Present University of Chicago Department of Medicine, Hematology/Oncology Section, Chicago, Illinois Proposed experiments for the direction of future research Managed, phenotyped and genotyped a genetically manipulated mouse colony Performed experiments utilizing a variety of immunological and informatics techniques DNA Functionality Intern August 2009 – May 2011 Wheaton College Biology Department, Wheaton, Illinois Trained and managed four students in their study of non-coding SNP sequences Initiated and completed computer automation of project Efficiently reduced the duration of the project by two years Epstein-Barr Virus Intern September 2003 – May 2006 Coriell Cell Repositories, Camden, New Jersey Researched and synthesized data for proposal to Board of Directors Operated cutting edge microscopy and flow-cytometry machinery and analysis software Explained project effectively to knowledgeable experts and to laypersons EXPERIENCE Teacher’s Assistant Microbiology & Immunology Wheaton College Biology Department, Wheaton, Illinois Prepared lab materials and assisted students in their class work May 2010 – August 2010 Member and Student Chapter Representative August 2009 – May 2011 American Scientific Affiliation, Wheaton College Chapter, Wheaton, Illinois Participated in local chapter meetings and enlisted other students for membership Presentation of a biomedical ethics poster at the 2011 annual meeting Tutor and Mentor Wheaton College Biology Department, Wheaton, Illinois Assisted student learning in General Chemistry and Biology Coached student to achieve an “A minus” from a failing grade in two months Prepared curriculum and study guides for students August 2009 – May 2011 Club Member/Volunteer Sigma Delta Pi: Health Professions Club, Wheaton, Illinois Volunteered at free health clinic and blood drives August 2007 – May 2011 EXTRACURRICULAR ACTIVITIES Intramural Men’s Soccer Player Wheaton College, Wheaton, Illinois Participated with a team to achieve the championship title October 2007 Tour Coordinator & Fund Raiser August 2008 - May 2009 Wheaton College Gospel Choir, Wheaton, Illinois Managed students to organize events, raised money to travel internationally to serve underprivileged communities TECHNICAL/LABORATORY SKILLS Molecular and Cell Biology Conventional PCR, cell fractionization by gradient centrifugation (fycoll), immuno-magnetic selection of human primary hematopoietic cell populations Flow cytometry (LSR II) Cell culture of established cell lines Functional experiments: proliferation assays (CFSE), quantifying apoptosis after agonist culturing Animal Immunology and Maintenance Transgenic mouse colony: breeding, phenotyping, genotyping, selective mating, upkeep, tail-vein bleeding, whole-mouse imaging, organ preservation for immunohistochemistry, non-survival surgery, organ extraction for experimentation (bone marrow, spleen) Microscopy Light microscopy of in vitro cultures Informatics Microsoft Office: Word, Excel, Power Point Some experience with C++ and PHP programming, database management PUBLICATIONS Cell Collections (Periodical) May 2007 Tretina, Kyle, and Karen Fecenko-Tacka. "Interleukin-10 Enhances Epstein-Barr Virus Transformation in Human Lymphocytes." Cell Collections (2007): 16-18. SELECTED POSTERS AND ORAL PRESENTATIONS Hematology/Oncology Laboratory Research Meeting, November 2011: University of Chicago, Chicago, IL; PowerPoint Presentation: “Establishment of a Knock-out Mouse Model to Determine Hematopoietic Cell Subsets Required for Agonist Effects on GVHD, GVT.” Annual American Scientific Affiliation Meeting (ASA). January 2011: North Central College, Naperville, IL; Poster Presentation: “Race and Medicine: Enelapril.” Kyle Tretina, Laura Frantz Annual Biology Symposium, January 2011: Wheaton College, Wheaton, IL; Poster Presentation: “Functionality of the Non-coding DNA on Human Chromosome 16.” Kyle Tretina