Fig. S1 Scanning electron micrographs images showing the abnormalities growth of G. boninense at the region of
inhibition. a Control hyphae. b Lysed hyphal tip. c Folding back of hyphae. d Hyphal swelling and curling. Red
arrows indicate the abnormal growth highlighted. White arrows indicate normal radial growth of fungus. Scale bar
indicates 10 µm.
a
c
b
d
Fig. S2 HPLC analysis of ethyl acetate extracts obtained from single culture and the inhibition zone of cross-plug
culture on ISP 4 medium. The eluate was monitored at 200 nm. a Ganoderma boninense. b Streptomyces sanglieri
strain AUM 00500. c Cross-plug culture of S. sanglieri strain AUM 00500 and fungus. Peaks labelled with retention
times of 2.8 and 3.8 min represent cycloheximide and actiphenol, the remaining peaks indicate agar constituents and
solvent ethyl acetate.
a
b
c
Fig. S3 The strong antagonist of fungus, Streptomyces sanglieri strain AUM 00500, produces two antifungal
metabolites. a Total ion chromatogram at UV wavelength 200 nm. b and c UV/Vis spectra of the peaks A and B,
extracted from the inhibition zone of cross-plug culture grown on ISP 4 medium. The peak (A) with a retention time
of 2.8 min had a UV spectrum identical cycloheximide,(B) with a retention time of 3.8 min had a UV spectrum
identical actiphenol.
a
A
b Peak#
Retention Time
B
:1
: 2.753
c Peak#
Retention Time
:2
: 3.765
Fig. S4 LCMS spectrums of two peaks were recorded in the HPLC elution profile of ethyl acetate extract of the
inhibition zone of cross-plug culture grown on ISP 4 medium, monitored at UV wavelength 200 nm. a The peak (A
in Fig. S3) at a retention time 2.8 min with reference spectrum cycloheximide (MSMS: precursor m/z 282.1). b The
peak (B in Fig. S3) at a retention time 3.8 min with reference spectrum actiphenol (MSMS: precursor m/z 276.1).
a
b