UCSF Lab Standard Operating Procedure Chemical Process, Name or Hazard Class: Copper Cyanide Please fill out the form completely. Insure all users have access to this SOP. Refer to instructions for assistance. Enter text. Department: Date SOP was written: Enter date. This lab specific SOP has been reviewed and approved by: Principal Investigator Name: Enter text. Principal Investigator Signature Lab Manager/Supervisor: Type of SOP: ☐ Process Enter text. ☒Hazardous Chemical ☐ Hazardous Class Purpose Copper cyanide (Cuprous cyanide) is an acute toxin. Very harmful or fatal if swallowed, inhalation, or absorbed through skin. Causes eye and skin irritation and may be absorbed through skin contact. May cause respiratory and digestive tract irritation. May cause central nervous system effects and blood abnormalities. May be metabolized to cyanide which then inhibits cytochrome oxidase to impair cellular respiration. Copper cyanide is a useful reagent in organic synthesis and is used for electroporation of copper. Physical & Chemical Properties/Definition of Chemical Group CAS#: 544-92-3 Class: Acute toxin Molecular Formula: CuCN Form (physical state): powder Color: off white/pale yellow-green Copper Cyanide 1 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Boiling point: NA Potential Hazards/Toxicity Copper cyanide is an acute toxin, and extremely hazardous or fatal in case of ingestion, inhalation or absorbed through skin. May be absorbed through the skin in harmful amounts and is an irritant to skin and eye. If absorbed, causes symptoms similar to those of inhalation and ingestion. May be metabolized to cyanide which in turn acts by inhibiting cytochrome oxidase impairing cellular respiration. Acute: Ingestion may cause gastrointestinal irritation with nausea, diarrhea, and vomiting. May cause tissue anoxia with symptoms of weakness, confusion, weak and irregular heart beat, collapse, unconsciousness, convulsions and death. May cause cyanosis, bloody stools, low blood pressure, jaundice and coma and produce systemic toxic effects to the kidney and liver and central nervous excitation. Inhalation may cause respiratory tract irritation and nausea, headache, dizziness, unconsciousness and coma. Chronic: Repeated exposure to copper cyanide may cause central nervous system damage. May be metabolized to cyanide, impairing cellular respiration. Chronic exposure may lead to the development of a "cyanide" rash, characterized by itching, vesicular eruptions, and may be accompanied by secondary infections. May also produce loss of appetite, headache, weakness, nausea, dizziness, and upper respiratory tract irritation. . Engineering Controls Handle using a chemical fume hood with good ventilation and electrically grounded lines and equipment. Personal Protective Equipment (PPE) Respirator Protection If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://www.ehs.ucsf.edu/respiratory-protection-program) Hand Protection Gloves must be worn. Use proper glove removal technique to avoid any skin contact. NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Copper Cyanide. Refer to glove selection chart from the links below: http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf OR http://www.allsafetyproducts.biz/page/74172 OR http://www.showabestglove.com/site/default.aspx OR http://www.mapaglove.com/ Copper Cyanide 2 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles. Face shield is also recommended. Skin and Body Protection Flame resistant lab coats must be worn and be appropriately sized for the individual and buttoned to their full length. Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed. Hygiene Measures Wash thoroughly and immediately after handling. Remove any contaminated clothing and wash before reuse. First Aid Procedures If inhaled Move into the fresh air immediately and give oxygen. If not breathing give artificial respiration. Seek medical attention immediately. In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash any contaminated clothing before reuse. Thoroughly clean shoes before reuse. Seek medical attention immediately. In case of eye contact Check for and remove any contact lenses. Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician. Seek immediate medical attention and continue eye rinse during transport to hospital. If swallowed Do NOT induce vomiting unless directed by medical personnel. Never give anything by mouth to an unconscious person. Seek medical attention immediately. Special Handling and Storage Requirements Precautions for safe handling: Avoid contact with skin and eyes and inhalation. Keep away from sources of ignition, heat, sparks, or flame. Take caution against static discharges and avoid mechanical shock and friction. Conditions for safe storage: Keep container tightly closed in a cool, dry, and well-ventilated. Keep away from incompatible materials and conditions. Store in original container. Store away from heat sources and in a flame proof area. Keep cool and protect from sunlight. Spill and Accident Procedure Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) Copper Cyanide 3 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.). Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up. Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance. Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends. Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends. Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone) Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours. Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays). Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours. Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC). Decontamination/Waste Disposal Procedure Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste. Safety Data Sheet (SDS) Location Copper Cyanide 4 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Online SDS can be accessed at http://www.ehs.ucsf.edu/safety-data-sheet-sds-1 Protocol/Procedure Quantities covered by this SOP: ______ (g , ml) to _______ (g, ml) Temperature range covered by this SOP: __ °C – __ °C General Overview and Purpose: Enter the experimental purpose Procedure: Enter experimental procedure. You can copy procedure from your lab notebook or from literature. NOTE Any deviation from this SOP requires approval from the Principal Investigator. Copper Cyanide 5 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)