Research Shows For The First Time That Lyme Disease Bacterium Does Not Require
Iron To Infect Host
ScienceDaily (June 6, 2000) — ATHENS, Ga.
-- New research from scientists at the University of Georgia, just published in the journal
Science, demonstrates that Borrelia burgdorferi, the bacterium that causes Lyme disease in
humans, is the first pathogenic bacterium identified that does not need or use iron.
"All bacterial pathogens described to date have developed specialized systems to acquire
iron from their hosts,"said microbiologist Frank Gherardini. "Current dogma states that to be
successful in humans, bacteria must overcome strict iron limitations that the human body
imparts on them. Although iron is abundant in humans, the amount of free iron is well below
the levels required to support the growth of most bacteria. To our surprise, we found that B.
burgdorferi doesn't even require iron. In fact, iron is extremely toxic to it."
Understanding how these bacteria are able to successfully colonize humans and cause
disease will ultimately lead researchers to more effective ways to prevent and control the
disorder.
Some 30 years ago, doctors were puzzled by a large number of arthritis cases in children
who lived in and near Lyme, Conn. After exhaustive study, they discovered that the disorder,
which has symptoms that vary in kind and severity, was caused by a bacterium transferred to
humans from the bite of the deer tick, Ixoides scapularis. Since then, there have been major
efforts to understand the bacterium and its life-cycle, and Gherardini's lab has been involved
for a number of years, publishing many papers on the subject. Gherardini's graduate student
James E. Posey was co-author on the paper published today in Science.
Borrelia burgdorferi is a spiral-shaped organism called a spirochete. Some scientists believe
that Lyme disease came from Europe a century ago but was only recently detectable when it
became more common. A resurgent deer population-along with mice, the reservoir host for
the bacterium, coupled with increased outdoor activities by humans, has helped insure
increased occurrence and spread of the disease.
The first symptom of Lyme disease is usually a red rash that often resembles a bull's eye.
This rash, which can be small or cover a person's entire back, appears within a few weeks of
a tick bite when the spirochete is introduced into the human host. The infection spreads to
different parts of the body and is often accompanied by such symptoms as fatigue, body
aches, headache, fever and a stiff neck. Treatment with antibiotics in early stages of the
disease is effective in most cases.
The Lyme disease microbe is extremely difficult to isolate or culture, so most doctors look for
evidence of the rash at the site of the tick bite or antibodies to B. burgdorferi in the blood.
Despite available tests and therapies, many cases go undiagnosed for months. Humans with
late-stage Lyme disease are largely out of luck, and doctors can only treat the symptoms.
Though people don't die from Lyme disease, the attendant joint discomfort and other
symptoms can create ongoing misery. That's why laboratories are still working hard to
understand the ways in which Borrelia infects and survives in its hosts.
Until recently, Gherardini said, standard wisdom held that all bacterial pathogens require iron.
The new finding probably doesn't indicate that Borrelia evolved, though natural selection, a
life-cycle designed not to need iron. On the other hand, natural selection pressure on the
entire genome clearly made it possible for Borrelia to exist without iron, though why remains
unclear.
The studies in Gherardini's lab were helped immeasurably by the fact that the genome for
Borrelia burgdorferi has been completed, so scientists know the location of the bug's genes
on its chromosomes-and what these genes do.
The proof the researchers needed came slowly and painstakingly. First, they showed that the
activities of several common iron-dependent enzymes were undetectable in cell extracts.
Second, they found no iron-containing proteins in the bacterium during an analysis of the
complete genome sequence. Third, B. burgdorferi grew normally in the presence of high
levels of iron-limiting compounds, and fourth, the bacterium doesn't appear to alter gene
expression in response to iron-limiting conditions. Finally, by using mass spectroscopy and
radioactive iron transport techniques, they found that the levels of iron inside the cells of B.
burgdorferi are 1,000-fold less than those measured in other pathogenic bacteria.
"Uptake experiments using iron suggest that B. burgdorferi does not transport iron and there
are less than five atoms of iron per cell," said Gherardini. To date, Lactobacillus planatarum,
a free-living soil bacterium, is the only other organism for which experimental data
demonstrate that iron is not required for growth. Apparently, B. burgdorferi belongs to a
unique category of pathogenic bacteria that use a novel strategy to overcome iron limitation
in the human host.
Just how this new discovery can be used in developing either new vaccines or new therapies
for those infected with Lyme disease is not yet clear. While a vestigal iron pathway may still
exist in Borrelia burgdorferi, simply adding iron to a diet or as a medical therapy would do
little good. Still, because other metals such as manganese are used by the bacterium, the
discovery of how iron is unneeded by the bug could point toward an entirely new way to limit
the occurrence and severity of the disease in humans.
"The ultimate goal of the bacterium is not to wipe out the host," says Gherardini. "It's just to
find another place to live. This should tell use a lot about how they do that."
The good news is that probably far less than 1 percent of all ticks are infected with B.
burgdorferi, though in some areas, more than half harbor the microbe. Gherardini said
anecdotal evidence is that in Georgia, few, if any, strains of B. burgdorferi have been isolated
from ticks.
If you discover a tick on your body, pull it out gently with tweezers, being careful not to
squeeze the tick's body, then apply an antiseptic to the bite. Studies by National Institutes for
Health-supported scientists indicate that a tick must be attached to its host for many hours to
transmit the Lyme disease bacterium.