RODENT ENDPOINT PCR/RT-PCR FORM (D-1-M)
Please print and complete this form and forward (via e-mail or fax to MDS/RRDA. We will contact you
for a detailed quotation.
Institution:
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Date Submitted:
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Services Requested:
Mouse Panels:
18 Tests Panel
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
Mycoplasma pulmonis
Sendai Virus
Mouse Hepatitis Virus
Pneumonia Virus of Mice
Minute Virus of Mice
Mouse Parvovirus
Theiler's GD-VII
Reo-3 Virus
Mouse rotavirus
Ectromelia (Poxvirus)
11.
12.
13.
14.
15.
16.
17.
Lymphocytic Choriomeningitis Virus
Polyoma virus
K Virus
Mouse Adenovirus
Mouse cytomegalovirus
Lactodehydrogenase Virus
Choice of Mouse Thymic Virus or
Mouse Parvovirus (MMV)
18. Choice of Hantaan virus or other
Mycoplasma spp. *
15 Tests Panel
1.
2.
3.
4.
5.
6.
7.
8.
Mycoplasma pulmonis
Sendai Virus
Mouse Hepatitis Virus
Pneumonia Virus of Mice
Minute Virus of Mice
Mouse Parvovirus
Theiler's GD-VII
Reo-3 Virus
9.
10.
11.
12.
13.
14.
15.
Mouse rotavirus
Ectromelia (Poxvirus)
Lymphocytic Choriomeningitis Virus
Polyoma virus
K Virus
Mouse Adenovirus
Mouse cytomegalovirus
13 Tests Panel
1.
2.
3.
4.
5.
6.
7.
Mycoplasma pulmonis
Sendai Virus
Mouse Hepatitis Virus
Pneumonia Virus of Mice
Minute Virus of Mice
Mouse Parvovirus
Theiler's GD-VII
8.
9.
10.
11.
12.
13.
Reo-3 Virus
Mouse rotavirus
Ectromelia (Poxvirus)
Lymphocytic Choriomeningitis Virus
Polyoma virus
K Virus
9.
10.
11.
12.
13.
14.
15.
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse Adenovirus
Reovirus 3
Theiler’s murine encephalomyelitis
virus
7.
8.
9.
10.
11.
Rat cytomegalovirus
Sendai Virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Rat Panels:
15 Tests Panel
1.
2.
3.
4.
5.
6.
7.
8.
Mycoplasma spp.
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Rat Parvovirus
Lymphocytic Choriomeningitis Virus
Rat cytomegalovirus
Sendai Virus
11 Tests Panel
1.
2.
3.
4.
5.
6.
Mycoplasma spp.
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Rat Parvovirus
Lymphocytic Choriomeningitis Virus
Hamster Panel:
11 Tests Panel
1.
2.
3.
4.
5.
6.
Mycoplasma spp.
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Hamster Parvovirus
Lymphocytic Choriomeningitis Virus
7. Mice minute virus
8. Theiler’s murine encephalomyelitis
virus
9. Sendai virus
10. Reovirus 3
11. Hantaan virus
Other animal pathogens
Rotavirus, equine
Rotavirus, feline
Rotavirus, canine
Rotavirus, bovine
Rotavirus, porcine
Equine influenza virus
Equine protozoal myeloencephalitis (Sarcocystis neurona)
Samples (For multiple samples, please complete Form D-3.)
What type of sample will be submitted? (see recommendations page 3)
Sample source:
human
rat
mouse
other
Cell culture (flask size
; number of flasks
; passage #
Cell pellet (number of cells
; lot #
)
Tissue (type
; amount
)
DNA (concentration
; volume
; total amount
ng)
RNA (concentration
; volume
; total amount
ng)
Lysis buffer (if applicable)
Special components of buffer (if applicable)
)
Recommended Sample Size for Endpoint PCR
Sample
Cultured cells
Liver
Brain
Lung
Heart/muscle
Kidney
Spleen
Plant leaf
Mouse tail
Blood
Buffy coat
DNA
RNA
Other
Amount
5 x 10^6
25 mg (~2mm3)
25 mg
25 mg
25 mg
25 mg
10 mg
100 mg
0.8-1.2 cm
1 ml
50 ul
50 ng/reaction
100 ng RNA/reaction
Call
Recommended Shipping Conditions
Plasmid DNA
Liquid = A, W, D; pellet A
PCR products
Liquid = A, W, D; pellet A
Primers
Liquid = A, W, D; pellet A
cDNA or genomic libraries
With DMSO = D; without DMSO = W
RNA
D
Bacterial colonies
A, W
Stab culture
A
Cell banks
D
Glycerol stocks
D
Tissues
D
Blood
W
A = ambient temperature
W = wet ice
D = dry ice