Dr. Aamir Ali
Jr. Scientist
_______________
Enteric Pathogens Laboratory, Health Biotechnology Division,
National Institute for Biotechnology and Genetic Engineering, (NIBGE),
P.O. Box 577 Jhang Road, Faisalabad, Pakistan (www.nibge.org)
Assistant Professor NIBGE Campus - PIEAS University, Islamabad.
Higher Education Commission, Pakistan approved supervisor for MPhil and PhD studies.
Date of Birth:
Phone:
Email:
October 6th 1979.
+92(41)2651475, Ext.245, Fax: +92(41)2651472, Mobile: +923336550569
myaamirali@yahoo.com, aamirali@nibge.org
QUALIFICATIONS
 PhD Biotechnology (2009) from Quaid-i-Azam University Islamabad with partial
research work at International Vaccine Institute (IVI) Seoul, South Korea.
 MPhil Biotechnology (2004) from Quaid-i-Azam University Islamabad.
 Doctor of Veterinary Medicine (DVM), (2001) from University of Agriculture Faisalabad.
RESEARCH INTERESTS AND EXPERTISE









Isolation, purifications and characterization of bacterial pathogens
Detection of drug resistance and virulence factors of bacterial pathogens
Basic molecular biology and biotechnology techniques
Multiplex polymerase chain reaction
Purification of polysaccharide antigens from bacterial cultures
Spectrophotometery and chromatography techniques
Preparation of conjugate (polysaccharide-protein) vaccine candidates
Enzyme linked immuno sorbant assay (ELISA)
Animal trials of vaccine candidates
RESEARCH PUBLICATIONS:
1. Ali A, An SJ, Cui C, Haque A, Carbis R. Preparation and evaluation of immunogenic
conjugates of Salmonella enterica serovar Typhi O-specific polysaccharides with diphtheria
toxoid. Hum Vaccines, 2012; 8: 189-193.
2. Ali A, Haque A, Haque A, Sarwar Y, Mohsin M, Bashir S, Tariq A. Multiplex PCR for
differential diagnosis of emerging typhoidal pathogens directly from blood samples.
Epidemiol Infect, 2009; 137(1): 102-107.
3. Ali A, Haque A, Sarwar Y, Haque A, Mohsin M, Afzal A, Ifikhar T, Tariq A. Nested PCR based
diagnosis of Salmonella enterica serovar Paratyphi A directly from blood samples. Pak J Med
Sci, 2008; 24(4): 545-549.
4. Baker S, Sarwar Y, Aziz H, Haque A, Ali A, Dougan G, Wain J, Haque A. Detection of Vi
negative Salmonella enterica serovar Typhi in the peripheral blood of typhoid patients in the
Faisalabad region of Pakistan. J Clin Microbiol, 2005; 43(9): 4418–4425.
5. Habeeb MA, Sarwar Y, Ali A, Salman M, Haque A. Rapid emergence of ESBL producers in E.
coli causing urinary and wound infections in Pakistan. Pak J Med Sci, 2013; 29(2): 540-544.
6. Salman M, Ali A, Haque A. A novel multiplex PCR for detection of Pseudomonas aeruginosa:
A major cause of wound infections. In press Pak J Med Sci, 2013; 29(4):
7. Iftikhar T, Butt A, Nawaz K, Sarwar Y, Ali A, Mustafa T, Haque A. Genotyping of rotaviruses
detected from hospitalized children from Faisalabad region, Pakistan. J Med Virol, 2012; 84:
2003-2007.
8. Tariq A, Haque A, Ali A, Habeeb MA. Salman M, Sarwar Y. Molecular profiling of
antimicrobial resistance and integron association of MDR clinical isolates of Shigella species
from Faisalabad, Pakistan. Can J Microbiol, 2012; 58: 1047-1054.
9. Afzal A, Sarwar Y, Ali A, Haque A. Current status of fuoroquinolone and cephalosporin
resistance in Salmonella enterica serovar Typhi isolates from Faisalabad, Pakistan. Pak J
Med Sci, 2012; 28(4): 602-607.
10. Bashir S, Haque A, Sarwar Y, Ali A, Anwar I. Virulence profile of different phylogenetic
groups of locally isolated community acquired uropathogenic E. coli from Faisalabad region
of Pakistan. Ann Clin Microbiol Antimicrob, 2012; 11:23.
11. Raza A, Sarwar Y, Ali A, Jamil A, Haque A, Haque A. Effect of biofilm formation on the
excretion of Salmonella enterica serovar Typhi in feces. Int J Infect Dis, 2011; 15(11): e747e752.
12. Jabbar A, Ali A. Tawab A, Haque A, Iqbal M. Fatty acid profiling of lipid A isolated from
indigenous Salmonella Typhi strain by Gas Chromatography–Mass Spectrometry, Journal of
The Chemical Society of Pakistan, 2013, In press (accepted for publication).
13. Bashir S, Sarwar Y, Ali A, Mohsin M, Saeed MA, Tariq A, Haque A. Multiple drug resistance
patterns in various phylogenetic groups of uropathogenic E. coli isolated from Faisalabad
region of Pakistan. Braz J Microbiol, 2011; 42: 1278-1283.
14. Mohsin M, Haque A, Ali A, Sarwar Y, Bashir S, Tariq A, Afzal A, Iftikhar T, Saeed MA. Effect of
ampicillin, gentamicin and cefotaxime on release of Shiga toxins from Shiga toxin producing
Escherichia coli isolated during a diarrhea episode in Faisalabad, Pakistan. Foodborne
Pathog Dis, 2010; 7(1): 85-90.
15. Saeed MA, Haque A, Ali A, Mohsin M, Bashir S, Tariq A, Afzal A, Iftikhar T, Sarwar Y. A profile
of drug resistance genes and integrons in E. coli causing surgical wound infections in the
Faisalabad region of Pakistan. J Antibiot, 2009; 62: 319-323.
16. Haque A, Haque A, Sarwar Y, Ali A. Multiplex PCR for drug resistance against standard
antityphoid drugs in blood samples of typhoid patients (Abst). FEBS J, 2006; 273(S-1): 74-75.
17. Saeed MA, Haque A, Ali A, Mohsin M, Bashir S, Tariq A, Afzal A, Iftikhar T, Sarwar Y.
Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound
infections. J Infect Dev Ctries, 2009; 3(9): 667-670.
18. Mohsin M, Hussain A, Butt MA, Bashir S, Tariq A, Babar S, Ali A, Haque A, Sarwar Y, Haque
A. Prevalence of Shiga Toxin-Producing Escherichia coli in diarrheal patients in Faisalabad
region of Pakistan as determined by multiplex PCR. J. Infect Developing Countries, 2007;
1(2): 164-169.
19. Bashir A, Haque A, Bashir S, Ali A, Sarwar Y, Haque A. Molecular Analysis of Drug Resistance
in Clinical Isolates of MDR Salmonella enterica Serovar Typhi in Faisalabad, Pakistan.
Pakistan J Zool, 2009; 41(5): 363-369.
20. Jamil M, Bashir S, Mohsin M, Tariq A, Bashir A, Haque A, Sarwar Y, Ali A, Haque A.
Differentiation of common gram negative pathogens by PCR-ribotyping. Pak J Med Sci,
2007; 23(2): 1-5.
21. Haque A, Haque A, Sarwar Y, Ali A, Bashir S, Tariq A, Mohsin M. Identification of drug
resistance genes in clinical isolates of Salmonella typhi for development of diagnostic
multiplex PCR. Pak J Med Sci, 2005; 21(4): 402-407.
22. Bashir S, Haque A, Ahmad N, Raza A, Haque A, Sarwar Y, Ali A, Bashir S, Mohsin M, Ahmad
I, Ahmad J. Significant incidence of typhoid in Hepatitis C patients. Professional Med J, 2005;
12(3): 287-294.
Sequence Submissions: NCBI Genbank, with the accession numbers JN717229 to JN717232.
RESEARCH TRAININGS
1. Member Organizing Committee of “National Training Course on Handing and Use of
Laboratory Animals for Biotechnology and Biomedical Research” April, 2-4, 2013 at NIBGE,
Faisalabad.
2. Member Organizing Committee of “Two-day symposium on Dengue Fever: Virology and
Vector Control” Jan 31st – Feb 1st, 2013 at NIBGE, Faisalabad.
3. Participated in “2nd Regional Dengue Workshop” Oct 30th – Nov 3rd, 2012 held at NCP,
Islamabad.
4. Participated in “FAO/IAEA Workshop on Basic Surveillance and Control for Dengue Vectors
in Pakistan” May 28th to June 2nd 2012, at NCP, Islamabad.
5. Participated in “1st National Training Workshop on Chromatography and Mass
Spectrometry” April 2-6, 2012 at NIBGE, Faisalabad.
6. Member Organizing Committee of “Three-day workshop on Molecular Diagnosis of Dengue
Virus” February 1-3, 2012 at NIBGE, Faisalabad.
7. Participated in “31st International Training Course of Asia-Pacific International Molecular
Biology Network-Asian Molecular Biology Organization” June 2-6, 2008 at International
Vaccine Institute (IVI), Seoul, South Korea.
8. International Research Support Initiative Program (IRSIP) Training (8 months) sponsored
by Higher Education Commission (HEC), Islamabad on preparation of Salmonella OSP-DT
conjugate vaccine candidates against human typhoid and paratyphoid (Feb-2007 to Oct2007) at International Vaccine Institute (IVI), Seoul, South Korea.
9. Participated in “17th IVI International Advanced Course on Vaccinology in Asia-Pacific
Regions” July 7-12, 2007 at International Vaccine Institute (IVI), Seoul, South Korea.
10. Participated as laboratory instructor in “Workshop on Molecular Detection and Genotyping
of HBV/HCV by PCR methods and Quality Assurance Issues” December 20-24, 2004 at
NIBGE, Faisalabad.
11. Participated in “Seminar/Workshop on vaccine development, production and utilization in
developing countries. Issues, prospects and implications for Pakistan” August 8-10, 2003 at
Agha Khan University Karachi.
12. Participated in “Regional Seminar on prevalent and newly emerging poultry diseases” April
15-16, 2002 at University of Agriculture Faisalabad.
PRESENTATIONS
1. Molecular diagnosis and serotyping of dengue virus directly from blood samples. Aamir Ali,
Yasra Sarwar, Muhammad Ashraf, Ikhlaq Anwar and Abdul Haque. Poster Presentation at
International Conference on Biotechnology, Prospects and Challenges in Agriculture,
Industry, Health and Environment, April 22-25, 2013 at NIBGE, Faisalabad.
2. Purification and characterization of polysaccharide antigens from Salmonella Species for
future use in conjugate vaccine. Poster presented by Aamir Ali on scholarship at “Initiative
for Vaccine Research (IVR), Global Vaccine Research Forum” December 3-6, 2006 at
Bangkok, Thailand.
3. Nested-multiplex PCR; an effective tool for diagnosis of typhoidal diseases. Poster
presented by Aamir Ali at “2005 CAS-JSPS Asian Science Seminar on Recent Developments
in the Control of Infectious Diseases from Fundamental Understanding to Application”
December 20-23, 2005 at Tianjin, China.
3. Multiplex PCR for differential diagnosis of emerging typhoidal pathogens directly from
blood samples. Aamir Ali, Abdul Haque, Asma Haque and Yasra Sarwar. Oral presentation
at “2nd National Conference on Health Biotechnology” May 27-28, 2008 at Pakistan
Academy of Sciences, Islamabad.
4. Epidemiology of Shiga toxin-producing Escherichia coli in Pakistan as determined by
multiplex polymerase chain reaction. Mashkoor Mohsin, Asma Haque, Yasra Sarwar, Aamir
Ali, Saira Bashir, Ayesha Tariq and Abdul Haque. Poster presented at “National Symposium
on Biotechnology for Economic Prosperity” July 24-26, 2006 at Green Retreat Hotel,
Nathiagali.
5. Genetic study of drug resistance patterns of locally isolated Vi positive and Vi negative
strains of Salmonella typhi. Yasra Sarwar, Asma Haque, Saira Bashir, Aamir Ali, Ayesha
Tariq, Mashkoor Mohsin, Mahwish Jamil, Aasia Bashir and Abdul Haque. Poster presented
at “18th FAOBMB Symposium on Genomic and Proteomics in Health and Agriculture”
November 20-23, 2005 at Lahore (Got 1st Prize).
6. Development of a multiplex PCR for detection of Salmonella typhi and related pathogens.
Aamir Ali, Abdul Haque, Asma Haque and Yasra Sarwar. Oral presentation at “15th National
& 5th International Chemistry Conference” November 24-27, 2004 at Quaid-i-Azam
University, Islamabad.
STUDENTS SUPERVISION
Currently Supervising MPhil (Biotechnology) research of Ms. Asma Ahsan on preparation and
evaluation of polysaccharide protein conjugates of Salmonella 2013 from NIBGE Campus, PIEAS
University, Islamabad.
Supervised MPhil (Biotechnology) thesis of Ms. Sanam Faryal entitled as “Purification and
antigenicity evaluation of O-specific polysaccharides of locally isolated Shigella dysenteriae”
2013 from NIBGE Campus, PIEAS University, Islamabad.
Co-Supervised MS (Biotechnology) thesis of Ms. Iram Naz entitled as “Molecular detection and
drug resistance profile of non-lactose fermenting bacteria isolated from urinary tract and
wound infections” 2013 from Govt. College University, Faisalabad.
TEACHING
Currently teaching two MPhil Biotechnology Courses: “Cell & Molecular Biology” as Faculty
Member and “Recombinant DNA Technology” as Course Co-coordinator at NIBGE Campus,
PIEAS University, Islamabad.
Course Coordinator for the subject “Advance Immunology” for PhD studies at NIBGE Campus,
PIEAS University, Islamabad.