UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
Nitrobenzene
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Department:
Date SOP was written:
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This lab specific SOP has been reviewed and approved by:
Principal Investigator Name:
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Principal Investigator Signature
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Type of SOP:
☐ Process
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☒Hazardous Chemical
☐ Hazardous Class
Purpose
Nitrobenzene is a carcinogen and a reproductive toxin. Approximately 95% of nitrobenzene is consumed in
the production of aniline, which is a precursor to rubber chemicals, pesticides, dyes, explosives, and
pharmaceuticals. Other synthetic targets include azobenzene, nitrosobenzene, and phenylhydroxyamine. It
can also be used as a solvent.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 98-95-3
Class: Suspect Carcinogens and reproductive toxin
Molecular Formula: C6H5NO2
Form (physical state): liquid
Color: yellowish
Boiling point: 210.9 oC
Potential Hazards/Toxicity
Nitrobenzene
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
OSHA Hazards
Combustible Liquid, Carcinogen, Toxic by inhalation., Toxic by ingestion, Teratogen
Target Organs
Blood, Central nervous system, Male reproductive system., Liver, Spleen.
Other hazards which do not result in classification
Rapidly absorbed through skin..
Engineering Controls
Certified fume hood.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)
Hand Protection
Handle with nitrile gloves. Gloves must be inspected prior to use. Use proper glove removal technique
(without touching
glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in
accordance with applicable laws and good laboratory practices. Wash and dry hands..
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are
compatible with nitrobenzene
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI approved safety glasses or goggles.
Skin and Body Protection
Lab coats must be worn and be appropriately sized for the individual and buttoned to their full length.
Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves. Full
length pants and close-toed shoes must be worn at all times by all individuals that are occupying the
laboratory area. The area of skin between the shoe and ankle should not be exposed
Hygiene Measures
Avoid contact with skin, eyes, and clothes. Wash thoroughly and immediately after handling. Remove any
contaminated clothing and wash before reuse.
First Aid Procedures
If inhaled
If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician..
Nitrobenzene
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
In case of skin contact
Immediately flush skin with plenty of water for at least 15 minutes. If contact with liquid form, treat like
frostbite and do not remove any clothing. Seek medical attention immediately. Wash any contaminated
clothing before reuse. Thoroughly clean shoes before reuse.
In case of eye contact
Rinse thoroughly with plenty of water for at least 15 minutes. If possible, check for and remove any contact
lenses and consult a physician.
If swallowed
Do NOT induce vomiting unless directed by medical personnel. Never give anything by mouth to an
unconscious person. Seek medical attention immediately
Special Handling and Storage Requirements
Precautions for safe handling Avoid contact with skin and eyes. Avoid inhalation of vapour or mist.
Conditions for safe storage Keep container tightly closed in a dry and well-ventilated place. Containers
which are opened must be carefully resealed and kept upright to prevent leakage. Place in secondary
containment. Label container, secondary containment, and storage location with a Cancer Hazard or a
Reproductive Toxin label.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent
material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear
plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct
Nitrobenzene
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working
hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as
hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
Nitrobenzene
4
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)