LAB CASE NUMBER # 28-99912
Page 1 of 3
OFFICE OF THE
DISTRICT ATTORNEY
DR. ROSE LEIGH VINES
CHIEF DEPUTY
HORNET COUNTY
ALEXANDER GONZALES
DISTRICT ATTORNEY
DR. RUTH E. BALLARD
CRIME LABORATORY DIRECTOR
Date: 5/10/2010
HORNET COUNTY SHERIFF’S DEPARTMENT
13 Sting Street
Hornet’s Nest, CA. 95819
SUSPECT: R. Spear
VICTIM: J.B. Walker
ATTENTION: Detective Ronald Donaldson, Hornet County Sheriff’s Department
EVIDENCE EXAMINED:
This document reports the results of DNA testing on the listed items. Please see the report dated
3/10/2010 for the results of serological analyses performed on Items 22-1A and 27-1C.
Original items submitted to the laboratory:
22-1A
One tape-sealed envelope labeled “Fired 9 mm cartridge case from crime scene”
27-1C
One tape-sealed bag labeled “Black sock found in suspect’s bedroom”
Items analyzed in this report:
Item Number
22-1A-1
27-1C-1
38
Description
One tube labeled “28-999122-22-1A-1” (Extract from serological test for blood on
Item 22-1A)
One tube labeled “28-999122-1C-1” (Extract from serological test for blood on Item
27-1C)
One tape-sealed envelope labeled “Reference buccal swab from suspect R. Spear.”
EXAMINATION AND RESULTS:
Item 22-1A-1 contained approximately 0.2 mL of frozen extract remaining from a serological test for
human blood performed on Item 22-1A by analyst Doug Mason. DNA was extracted from the cells in the
entire serological extract, with a total yield of 4 ng of human DNA. One ng of the DNA extract was used
for nuclear DNA typing. The remaining DNA extract, labeled “22-1A-1 DNA,” remains for future DNA
analyses, if needed.
Item 27-1C-1 contained approximately 0.2 mL of frozen extract remaining from a serological test for
human blood performed on Item 22-1C by analyst Doug Mason. DNA was extracted from the cells in the
LAB CASE NUMBER # 28-99912
Page 2 of 3
entire serological extract, with a total yield of 22 ng of human DNA. One ng of the DNA extract was used
for nuclear DNA typing. The remaining DNA extract, labeled “27-1C-1 DNA,” remains for future DNA
analyses, if needed.
Item 38 was a buccal swab taken from suspect R. Spear for reference purposes. One half of the swab was
consumed to generate a DNA extract, with a total yield of 42 ng of human DNA. One ng of the DNA
extract was used for nuclear DNA typing. The remaining extract, labeled “38-DNA,” remains for future
DNA analyses, if needed. The remaining half of the original swab was also saved.
Nuclear DNA typing was performed using the Identifiler PCR kit, which amplifies the gender marker
amelogenin (AMEL) and fifteen short tandem repeat (STR) loci. The results are in the following table:
Item
Description
22-1A
Swab of stain on fired 9 mm cartridge case
27-1C
Stain from black sock
38
Suspect Spear reference
D8S1179
D21S11
D7S820
CSF1PO
D3S1358
THO1
D13S317
D16S539
D2S1338
D19S433
vWA
TPOX
D18S51
Amel
D5S818
FGA
12,13
29, 33.2
12,13
10,11
15,15
8,10
8,12
11,13
17,21
12.2, 16
16,17
10,11
12,14
XX
11,12
24,27
13,13
29,30
8,12
11,12
15,16
7,10
8,8
11,13
17,18
15,16
17,18
9,11
12,12
XY
10,11
21,24
12,13
29, 33.2
12,13
10,11
15,15
8,10
8,12
11,13
17,21
12.2, 16
16,17
10,11
12,14
XY
11,12
24,27
The DNA profile obtained from Item 22-1A indicates that the DNA is from a female and that Suspect R.
Spear is eliminated as its source.
The DNA profile obtained from Item 27-1C is the same as that of suspect R. Spear. This profile is
expected to occur, in a randomly selected individual, in approximately 1 in 7.7 septillion African
Americans, 1 in 640 sextillion Caucasians, and 1 in 360 sextillion Hispanics.
DISPOSITION OF EVIDENCE
Items 22-1A-1 DNA, 27-1C-1 DNA, and 38-DNA are being stored in a box in the laboratory’s long-term
evidence freezer. The box is labeled “LCN # 28-99912 DNA extracts.”
The remaining half of the evidence swab from Item 38 was returned to its original evidence envelope. The
envelope was then sealed and placed in the evidence kit for LCN #28-99912. The kit was sealed and is
currently being stored in the laboratory’s walk-in property room at 4 deg C.
LAB CASE NUMBER # 28-99912
Page 3 of 3
All of the items in evidence kit 28-99912 have been examined and the kit is ready for pick-up at your
earliest convenience. The DNA extracts will remain at the Hornet County Crime Laboratory for long-term
frozen storage.
Analyses Performed by:
Signature ________________________________ Print Name: Brian Cooper
Date: 5/10/2010
Technical Review by:
Signature ________________________________ Print Name: Ruth Ballard, PhD. Date: