Comparative Medicine
SOP #: 605. 02
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Biological Material Testing for Murine Pathogens
The intent of this standard operating procedure (SOP) is to describe biologic material testing
and sample submission. This SOP is intended for use by Principal Investigators (PIs), research
staff, the Institutional Animal Care and Use Committee (IACUC) and Comparative Medicine
(CM). This SOP is approved by the NUS IACUC. Any deviation must be approved by the
IACUC in advance.
TABLE OF CONTENTS
1.
2.
3.
4.
Introduction
Procedures
References
Appendix
1. INTRODUCTION
Biological material refers to cell
fluids, antibody preparations or
introduced into an animal, it is a
pathogens. Pathogens present in
that are inoculated with them.
lines, transplantable tumors, serum, tissues, body
hybridoma lines. When biological material is
potential source of contamination by adventitious
these materials have the potential to infect animal
Biological materials must be tested for specific pathogens from a CM approved
diagnostic laboratory (e.g. IDEXX RADIL, Charles River), prior to use in animals.
Please refer to Appendix A for the list of murine pathogens to be tested.
Allow 1-2 weeks from the time of sample submission until results are received.
The cost of biological material testing is currently borne by CM.
Non-animal users are not allowed to use the irradiator(s) located within a NUS/CM
vivarium for their biological materials. Please contact Life Sciences Institute (LSI) for
use of their irradiator.
2. PROCEDURES
a. Biologics that REQUIRE testing prior to in vivo murine use:
i. Murine-derived cell lines, transplantable tumors, serum, tissues, body
fluids, antibody preparations and hybridomas from originating from
rodent colonies outside of CM facilities.
SOP #: 605. 02
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ii. Non-murine (including human) derived cell lines, transplantable
tumors, serum, tissues, body fluids, antibody preparations and
hybridomas that have been passaged through rodents or have been
exposed to rodents outside CM animal facilities.
b. Possible exemptions from testing: Exemptions must be approved by CM.
i.
ii.
iii.
If the murine biological material is derived from donor animals
located in the same facility and room as the recipients (or approved
room within the same facility with the same health status).
Non-murine derived biological that have NOT been passaged
through rodent or exposed to murine products.
Commercially obtained biologicals for which the vendor can supply
negative screening results, satisfactory to CM.
c. Submitting samples:
i.
ii.
Contact CM at 65166410 or lacacca@nus.edu.sg to arrange testing
of biological material.
Submit samples to CM Vet Diagnostic Laboratory.
a. Complete submission form. Request for Pathogen Screening
for Biological Materials. (Appendix B)
b. Submit two 1ml cryovial of each cell line sample containing
a minimum of minimum of 2 x 106 cells. Cells can be in
pellet form, in freeze media, growth media, or PBS.
c. For liquid samples, submit two 1ml cryovials with at least
0.5ml.
d. Collect all samples aseptically
e. Label all samples with PI name and sample ID (cell line
designation).
f. Contact CM Vet Diagnostic Lab at 6516 6410 for further
assistance.
3. REFERENCES
 RADIL links:
http://www.radil.missouri.edu/Biological_Materials/Cell_Line_Authentication_Cell_Ch
eck_/index.html
 Charles River – Research Animal Diagnostics
http://www.criver.com/sitecollectiondocuments/rm_ld_d_sample_submission_info.pdf
 NUS OSHE: http://www.nus.edu.sg/osh/index.html
 Cornell- http://www.research.cornell.edu/care/documents/ACUPs/ACUP619.pdf
Revision #
.02
Author
Anna Acuna
Effective Date
17 July 2012
SOP #:
605.02
SOP #: 605.02
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4. APPENDIX
Appendix A
Mouse Pathogens
Mycoplasma spp.
Sendai virus
Mouse hepatitis virus
Pneumonia virus of mice
Minute virus of mice
Mouse parvovirus (MPV-1,2,3)
Theiler’s murine encephalomyelitis
virus
Murine norovirus
Reovirus-3
Mouse rotavirus
Ectromelia virus
Lymphocytic choriomeningitis virus
Polyoma virus
Lactate dehydrogenase-elevating
virus
Mouse adenovirus (MAD-1,2)
Mouse Cytomegalovirus
K virus
Mouse Thymic virus
Hantaan virus
Rat Pathogens
Kilham’s rat virus
Toolan’s H1 virus
Rat parvovirus
Rat cytomegalovirus
Rat coronavirus
Rat minute virus
Sialodacryoadenitis virus
Seoul virus
Mycoplasma spp
Pneumonia virus of mice
Lymphocytic
choriomeningitis virus
Sendai virus
Mouse adenovirus
Reovirus3
Rat Theilovirus
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Appendix B
Date: _________________
Please complete the information below:
Protocol #: _____________________
OSHE Risk Assessment #____________
Primary Investigator: _____________________
Email/Phone ______________________
Laboratory Contact: ______________________
Email/Phone______________________
In which animal facility and room will this biological material be used in?_______________
The biological material is:
1) Murine-derived:
Yes
No
If yes, is it derived from donor animals located in the same facility and room as the recipients (or
approved room within the same facility with the same health status)?
Yes
Facility and Room No.:
No
2) Non- Murine (including human) derived cell lines
Yes
No
If yes, has it been passaged through rodents or exposed to murine products?
Yes
No
If yes, was it passaged through rodents or exposed to murine products in the same room as the
recipients or approved room within the same facility with the same health status ?
Facility and Room No. :_________________________
Samples submitted
Name
Example: Hep93C
1.
2.
Description / Origin
Human hepatocellular carcinoma line
*Attach additional sheets if necessary
Additional comments:
Media
PBS