Editorial checklist for Nature Biology Papers Manuscript number: Author name: Title: Please complete this checklist in addition to the manuscript checklist at: http://www.nature.com/nature/authors/submissions/final/3d_MS_checklist.pdf and the reporting checklist at: http://www.nature.com/authors/policies/checklist.pdf. We need all 3 checklists to be completed in order to accept your paper. Please confirm that your manuscript meets all of the relevant points: 1. Formatting Yes The paper is within the allotted page limit (please use the provided macro to determine the length of your paper). The Methods section is provided in the SAME Word document as the main text, after the figure legends (numbering of references used only in the full methods section continues from the main references). The main figures do not contain tables as sub-panels; tables should be provided as separate display items. The main figures are supplied in an editable format. If additional figures and tables are submitted as Extended Data (ED): There are no more than 10 ED items. Each ED item is referenced in the main text. Each ED item fits on a single page. ED figure legends are listed sequentially at the end of the main text file, not in the Extended Data files. There are no titles or footnotes in the ED items. If Supplementary Information (SI) is submitted: Where possible, the SI is combined and submitted as a single PDF. SI figure legends are displayed under the individual figures. Harvard, MIT, Princeton, UCSF, Georgia Tech, University of Hawaiʻi at Mānoa, University of Rhode Island, Duke University, or Caltech authors: a signed waiver letter accepting our license to publish terms and conditions is provided. Video files are referred to as videos, not movies, throughout the text. Videos are created using H.264 encoding, with a standard aspect ratio of 16:9 (4:3 is second best) and are not compressed. Video titles (1 short sentence) and legends (max. 100 words) are submitted in an editable format. All relevant subject terms have been identified during online submission; multiple subject pathways, if relevant, are recommended to allow your paper to be found by as many readers as possible. Page 1 of 2 No NA 2. Reporting Standards Figure Legends If representative data are shown, is it indicated out of how many? Is n noted (exact number, not range)? Are error bars used and defined? Are biological or technical replicates defined? Are statistical tests named? Is there a P value for each test? For each cell line, is there a statement in the Methods for: the source of the cell lines whether the cell lines have been authenticated, and if so, by which method? whether the cell lines have been tested for mycoplasma contamination? Cell line misidentification Are any cell lines used listed in the database of commonly misidentified cell lines maintained by ICLAC and NCBI Biosample? If yes, has a scientific justification of their use been included in the Methods section? Yes No NA Animal Experiments – in Methods Section IRB approved? Statement about sample size choice? Statement about randomization? Statement about blinding? Sex of animals stated? Age of animals stated? Species and strains stated? Yes No NA Human Subjects – in Methods Section IRB approved? Informed consent? Consent to publish photographs? Trial registration for randomized clinical trials? Yes No NA Data Description and Deposition Small RNA sequences provided? Full chemical characterization (incl. structure) is provided for small molecules? Nature standard table used for X-ray crystallography/NMR structure data? Accession code provided for X-ray, NMR, and cryoEM structures? Accession numbers provided for protein, gene, genome, meta-genome or exome sequences; RNA-seq; ChIP-seq; microarray data; zoobank? Project ID supplied for MG-RAST? Yes No NA Page 2 of 2