:
McMaster University
Initial Issue Date:
Revision Date:
Title:
PCR
Cabinet
PCR Cabinet / Workstation – Standard
Operating Procedure
Approved by:
1.0
Section:
Document Number:
Page 1 of 3
Purpose:
This procedure outlines the steps to be followed in using a PCR Cabinet /
Workstation.
2.0
Scope:
2.1 This procedure applies to all students, staff and researchers.
3.0
Definitions:
UV Sterilizing PCR Cabinet/Workstation – Equipment that allows for the
preparation of PCR and other samples in an environment with low risk of sample
contamination via ultraviolet deactivation of DNA between experiments.
UV/Air Sterilizing Re-Circulator – Optional equipment on some units that allows
air to be re-circulated after either passing through a HEPA filter or a UV
sterilization process which eliminates airborne microbes.
4.0
Responsibility:
4.1 It is the responsibility of all students, staff and researchers to ensure they
receive proper hands-on training by staff of the facility before performing the
procedure on their own.
4.2 It is the responsibility of all students, staff and researchers to perform the
work according to the procedures enclosed in this document.
4.3 It is the responsibility of the USER to report any damage or malfunction of
this equipment to the emergency contact person listed.
You are required to leave a note on the equipment describing the
problem and include your name and date the problem occurred.
5.0
Related Policies/Procedures:

Preparation of samples for PCR
6.0
Equipment:

Personal protective equipment: latex/nitrile gloves, lab coat, UV protective
eyewear or face shield if needed.
____________________________________________________________________________
7.0 Action/Decision-making Framework:
PROCEDURE
STEPS
7.1 Pre-use inspection
7.2 Using cabinet
WORK INSTRUCTIONS
.1 Check:
 Condition of electrical cord
 UV light is off
 Keylock is off (if applicable)
RATIONALE
To prevent accidental
exposure of personnel
or samples to UV light.
.1 Internal plugs, if provided, may be used for
other equipment, which is to be
decontaminated between experiments.
.2 Equipment plugged into internal plugs
should not exceed the maximum
recommended amperage.
.3 Cabinet is not to be used in place of a
biological safety cabinet.
7.3 Operation of UV
light
.1 (If applicable) Ensure key is completely
inserted into the lock. Turn keylock ON.
.2 Set timer for appropriate decontamination
time.
.3 Turn keylock OFF when done.
7.4 White light
To prevent sample
exposure to UV light.
.4 NEVER commence work with UV light on.
UV can harm all
personnel in vicinity.
.4 Keep UV use to a minimum.
To minimize crazing of
acrylic panels.
.5 Use recommended procedures to measure
UV emissions at recommended intervals
To ensure adequate
decontamination is
occurring.
.1 Keep ON while working.
Prevents eye strain.
.2 May be on at any time, including
decontamination.
PROCEDURE
STEPS
7.5 Air Recirculation
WORK INSTRUCTIONS
RATIONALE
.1 Not applicable for all units.
.2 Turn ON.
.3 Keep air vents clear.
7.3 Cleaning and
Maintenance
For proper air
circulation.
.1 Cleaning of work station includes making
sure it is in the condition it was when you
first used it.
.2 Unplug before performing any cleaning or
maintenance.
.3 Clean spills promptly according to
appropriate spill protocol.
.4 Use a mild detergent and soft cloth.
Never use organic compounds, alcohols,
ammonia-based cleaners, or abrasive
materials.
.5 Clean dust from cord and plug pins at
least yearly.
.6 UV lights must be tested routinely and
bulbs changed as needed.
7.5 Safety
.1 All those using the equipment must wear
the appropriate personal protective
equipment. This should include lab coats
& gloves.
.2 The unit has built-in safety features.
Never attempt to override any safety
feature.
 The safety shut-off turns off the UV
light when the door is opened.
 The acrylic door is UV blocking (if at
least 0.5 inches thick).
 In the unlikely event of a malfunction,
NEVER expose eyes to UV light
without wearing UV blocking glasses.
8.0
As UV lights age, their
intensity diminishes,
which affects their
germicidal activity.
Documentation:
Even short exposure to
UV light can cause
damage to unprotected
eyes and skin.
9.0
References:
Equipment manual
10.0
Developed By in Consultation With:
FHSc. Safety Office