BioSc 321 Pathogenic Microbiology Exam 1 Due 9/18/2003 We discussed a new system for characterizing emerging and re-emerging disease agents (new-new, new-old, old-new, and old-old). Other than the organisms in Table 1-1, name one additional example of EACH category and explain why you placed it in that particular category. Numerous molecular techniques have been developed to identify bacterial pathogens. Select an appropriate technique for identifying a “new” bacterial pathogen that is causing an outbreak of pneumonia and explain your reason for choosing that technique. Would the same technique be used to track the transmission of the organism once it has been isolated, cultured and identified? Why or why not? If not, suggest and alternative technique and explain your reason for using that technique. Many aspects of modern living are associated with increased incidences of infectious disease such as increased incidences of Lyme disease resulting from building houses in areas frequented by wild animals. Describe five other examples of modern “conveniences” impacting infectious disease. Describe “sloppy feeding”. What would you expect to find in the “remnants” of sloppy feeding? What advantage does the host derive from having phagocytic cells that are “sloppy feeders”? Helicobacter pylori has been proven to cause gastric ulcers. One factor that may be critical for virulence of H. pylori may be it’s acid tolerance. An outer membrane protein (OmpX) of H. pylori is believed to impart acid tolerance in this organism. Describe how a molecular version of Koch’s Postulates may be used to verify that OmpX is a virulence factor of H. pylori. (Assume that there is a mouse model for studying H. pylori virulence.) Describe septic shock and explain the mechanism that leads to septic shock. What causes neutropenia and what is the impact of neutropenia on infectious diseases? Complete the table below by predicting the effects of a complete deficiency of each of the complement proteins listed at the top of the chart on the activities shown in the left hand column. Use the notation N = no inhibition; P = partial inhibition; C = complete inhibition. C1 C3 C4 C5 C9 Activation of alternative C3 convertase Activation of classical C3 convertase Activation of alternative C5 convertase Activation of classical C5 convertase Complement-mediated phagocytosis Complement-mediated inflammation Complement-mediated lysis Factor B C1INH