Envision Instrumentation for Users - QB3

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HTSF Instrumentation for Users

EnVision

®

Multilabel Plate Reader

Model 2104-0020

Technologies

The system reads all non-isotopic detection technologies in all major applications, including:

• Top and Bottom Read of Fluorescence

• Luminescence

Absorbance

Applications

EnVision is a multilabel reader for all major applications, including

Reporter gene assays

Cell applications

Genotyping

Enzyme assays

Signal transduction

Receptor ligand binding

Immunoassays

Quantification

Possible other future assays.

Counting modes and features included

EnVision counting modes are easily accessible. In the same protocol, even for the same well, you can freely use several different counting modes and labels .

Plate format:

96-well; 384-well; 1536-well

Temperature Control

To ensure reproducibility of cellular and enzyme kinetic assays and other assays that require a defined temperature range, the EnVision platform’s temperature control option lets you precisely regulate from +2°C to +45°C above ambient. Temperature control is uniform across the plate, and a top heating plate eliminates condensation problems.

Dispenser

The EnVision dispenser can be used with 1- to 384-well plates in all reading modes. It consists of two pump units, a magnetic stirrer and a heater, allowing precise delivery of reagents in 1

μL increments over a volume range of 2-475

μL. An adjustable dispense speed setting lets you optimize for viscosity, cell density and other assay requirements.

Plate Stacker

Available for 20 or 50 plates, interchangeable plate stacker magazines can easily be added to increase capacity and throughput. For kinetic assays, the grip-fed re-stacking function lets you maintain the order of plates in the stack, increasing the reliability of data.

Plate barcode

The barcode reader allows you to load barcode labeled plates, which are identified by the barcode reader. Codabar,

Code39, Interleaved 2 of 5, Code 128, UPC and EAN barcodes can be read.

Plate loading modes

Available in HTSF:

Manual reading (one plate at time)

Stacker loading (up to 50 plates per load)

Optical filters

A wide range of filters cover the wavelengths used in fluorometric and photometric applications. Below are 16 filters available in HTSF:

Barcode 217 243 229 214 207 206 102 319

Wavelength M590 M685 M595w M510 M460 M535 X485 P600

Barcode 103 318 105

Wavelength X355 P570 X531

302 227 112 118 303

P405 M650 X380 X620 P450

Application-specific mirror modules

User interchangeable application specific optical modules form a crucial part of the optical system. The optical modules include all application critical components; for example dichroicmirrors for specific labels. All applicationspecific optical modules are barcoded.

Top and bottom reading

Fluorescence readings (including FP and TRF) can be taken from the top or bottom. Top reading is the most efficient method when no lid is used because no plastic surface has to be penetrated. For adherent cells and lidded plates, below reading provides superior efficiency. It is a true epimode, in the sense that the both excitation and emission are directed to and from below. For top reading, four optical modules can be in place. Any of the them can also be used for below reading.

Below are 5 mirror modules available in HTSF.

Barcode

Wavelength?

401

B550

403

D505

405

D555

412

D400

420

D658

Ultra-Sensitive Luminescence technology makes the most of your precious cells and reagents

For the ultra sensitive luminescence, we have 5 types apertures (96-L1, 384-L1,1536-L1,1536-LP-L1 and 0L1/A1) for

96, 384 ad 1536 plates respectively. They are working with primary, stem or difficult-to-transfect cells presents a unique set of challenges. Optional ultra- sensitive luminescence boosts sensitivity by almost 40X vs. standard luminescence by bringing the detector closer to the sample —giving you more information from every cell.

Software that enhances both productivity and compliance

Powerful software makes it easy to take advantage of all the performance and versatility of the EnVision platform. An intuitive interface with pre-set user-specific protocols makes setting up the system fast and easy. For post-run data analysis, features such as automatic curve fitting and flexible interpretation of standards controls and unknowns gives the EnVision system the power to handle a broad range of assay types. The software integrates smoothly into your lab’s network and into lab automation and data management systems. For regulated environments, PerkinElmer’s

Enhanced Security mode provides technological controls and features that support 21 CFR Part 11 compliance.

Visit www.perkinelmer.com/EnVision for more information.

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