DNA DRY LAB
The following is the base sequence on one strand of a
DNA molecule:
A A T G C C A G T G G T T C G C A C
1. Give the base sequence of the complementary DNA strand.
TTACGGTCACCAAGCGTG
2. Draw this DNA molecule including sugars and phosphates.
3. Give the base sequence of the strand of mRNA. Read from
the original DNA strand.
Same as the answer for #1 except the “T”s are replaced with
“U”
4. Draw this RNA molecule including bases and sugars.
5. What protein fragment would this mRNA code for? Use
tables found in notes and assignments to list the amino
acid sequence.
Leu – Arg – Ser – Pro – Ser – Val
6. If the fourth nucleotide in the original DNA strand were
changed from G to C, what would the resulting mRNA look
like?
UUAGGGUCACCAAGCGUG
7. How would the amino acid sequence change?
words.
Describe in
The second amino acid would change from Arg to Gly
8. Will this protein to the same job the original protein
did?
No, if the amino acid sequence changes, the shape of the
protein changes and it will either be non functional or do
a different job.
9. If a G were added to the original DNA strand after the 3rd
nucleotide, what would the resulting mRNA look like? \
UUACCGGUCACCAAGCGUG
10.
List the amino acids this would code for. What type of
mutation is this? Will this protein to the same job the
original protein did?
Leu – Pro – Val – Thr – Lys – Lys – Arg – nothing
This is an insertion mutation. It is also considered a
“frame shift” mutation because all of the amino acids
changed after the addition. This protein will NOT do the
same job, see explanation above.
11.
If the 9th nucleotide in the original DNA strand were
changed from T to A, what would the resulting mRNA look
like?
UUACGGUCUCCAAGCGUG
12.
Describe the change in the amino acid sequence. Will
this protein to the same job the original protein did?
What type of mutation is this?
There would be no change in the sequence. This would be
considered a substitution mutation and the mutation is
silent. The protein WILL do the same job as the original
protein.