DNA DRY LAB The following is the base sequence on one strand of a DNA molecule: A A T G C C A G T G G T T C G C A C 1. Give the base sequence of the complementary DNA strand. TTACGGTCACCAAGCGTG 2. Draw this DNA molecule including sugars and phosphates. 3. Give the base sequence of the strand of mRNA. Read from the original DNA strand. Same as the answer for #1 except the “T”s are replaced with “U” 4. Draw this RNA molecule including bases and sugars. 5. What protein fragment would this mRNA code for? Use tables found in notes and assignments to list the amino acid sequence. Leu – Arg – Ser – Pro – Ser – Val 6. If the fourth nucleotide in the original DNA strand were changed from G to C, what would the resulting mRNA look like? UUAGGGUCACCAAGCGUG 7. How would the amino acid sequence change? words. Describe in The second amino acid would change from Arg to Gly 8. Will this protein to the same job the original protein did? No, if the amino acid sequence changes, the shape of the protein changes and it will either be non functional or do a different job. 9. If a G were added to the original DNA strand after the 3rd nucleotide, what would the resulting mRNA look like? \ UUACCGGUCACCAAGCGUG 10. List the amino acids this would code for. What type of mutation is this? Will this protein to the same job the original protein did? Leu – Pro – Val – Thr – Lys – Lys – Arg – nothing This is an insertion mutation. It is also considered a “frame shift” mutation because all of the amino acids changed after the addition. This protein will NOT do the same job, see explanation above. 11. If the 9th nucleotide in the original DNA strand were changed from T to A, what would the resulting mRNA look like? UUACGGUCUCCAAGCGUG 12. Describe the change in the amino acid sequence. Will this protein to the same job the original protein did? What type of mutation is this? There would be no change in the sequence. This would be considered a substitution mutation and the mutation is silent. The protein WILL do the same job as the original protein.