1 Supporting Information 2 Table S2. List and brief definitions of 34 flow metrics calculated in this study. Metric Definition MA3 CV in daily flows MA5 Mean daily flow/median daily flow MA11 Ratio of 25th/75th percentiles in daily flows/median daily flow MA41 Mean annual flow/catchment area ML4 Mean minimum monthly flow for April ML17 7-day minimum flow/mean annual daily flow averaged across all years ML18 CV in ML17 ML21 CV in annual minimum flows averaged across all years MH8 Mean maximum monthly flow for August MH10 Mean maximum monthly flow for October MH14 Median of the highest annual daily flow/the median annual daily flow averaged across all years MH16 Mean of the 10th percentile flow/median daily flow across all years FL1 Mean frequency of low flow (25th percentile) FL2 CV in frequency of low flow (25th percentile) FL3 Total number of low flow spells (threshold equal to 5% of mean daily flow)/the record length in years FH2 CV in frequency of high flow (75th percentile) FH3 Mean frequency of high flow (3 times median daily flow) FH6 Mean frequency of high flow (3 times median flow over all years) FH7 Mean frequency of high flow (7 times median flow over all years) DL13 Mean annual 30-day minimum/median flow DL16 Mean duration of low flow (25th percentile) DL17 CV in mean duration of low flow (25th percentile) DL18 Number of zero-flow days DH13 Mean annual 30-day maximum/median flow DH15 Mean duration of high flow (75th percentile) DH16 CV in DH15 DH20 Mean duration of high low (25th percentile of median flows) TA1 Constancy (Colwell 1974) TL2 CV in the Julian date of the 1-day annual minimum flow TH3 Maximum proportion of the year (number of days/365) during no 1.67-year floods have ever occurred RA5 Ratio of days where the flow is higher than the previous day RA6 Median of difference between 2 consecutive days with increasing flow RA8 Number of negative and positive changes in water conditions from one day to the next RA9 CV in RA8 See the “all streams” column in Table III in Olden & Poff (2003) for the complete list. 3 a 4 Abbreviations for the metrics are the same as those in Olden & Poff (2003) to maintain 5 consistency. SD, standard deviation; CV, coefficient of variation. 6 DL16 DH20 DH15 DH13 PC2 (15%) FL2 MA5 MH14 MA3 FH7 MH16 MH8 MH10 DL17 RA9 SDdis MA41 Meandis TH3 ML4 FH6 TL2 TA1 FH2 DL13 ML17 MA11 RA6 DL18 FL3 DH16 FH3 ML18 ML21 RA5 FL1 RA8 7 PC1 (33%) 8 Fig. S2. Results of principal component (PC) analysis on 36 flow metrics calculated in 9 the present study (See Table S2 for full definitions of the metrics). The first and second 10 PCs together explained 48.8% of the variation in flow metrics.