Annex 1: Analysis by liquid chromatography-mass spectrometry (LC-MS) of a small
fragment of material of Bocoa mollis (Swartzieae) from RBG Kew’s Herbarium
collections reveals the very unusual amino acids thought to be unique to Ateleia and
Cyathostegia. Traditionally Bocoa has not been considered very closely related to
these genera but DNA sequence analysis now suggests that some species of Bocoa,
such as B. mollis, may have affinities to Ateleia whereas others, such as B.
prouacensis, do not. LC-MS fails to detect the unusual amino acids in B. prouacensis
supporting the new hypothesis on relationships.
H
N
HOH2C
NH2
COOH
COOH
HOOC
100
NH2
Ateleia herbert -smithii
80
60
COOH
Relative Abundance
40
20
0
100
. Bocoa mollis
80
60
40
20
0
100
Bocoa prouacensis
80
60
40
20
0
0
2
4
6
8
10
12
14
Time (min)
16
18
20
22
24
26