Biotechnology Pre/PostTest
Name ________________________________________
Date __________________
MULTIPLE CHOICE
_____ 1) Which of the following is an example of a product easily found in nature?
A) Antibiotic
B) GMO
C) Recombinant DNA
D) All of the above
_____ 2) In a biotechnology lab, tap water is best used for
A) making buffers.
B) washing glassware.
C) the final rinse when washing glassware.
D) All of the above
_____ 3) The order of amino acids on a polypeptide chain is encoded by
A) DNA
B) mRNA
C) rRNA
D) Regulatory proteins
_____ 4) How do DNA molecules vary from one species to another?
A) The type of nitrogenous bases they contain
B) Number of genes and non-coding regions
C) The way nitrogenous bases pair
D) The directionality of the strand
_____ 5) What is Agar?
A) Solid media on which bacteria grow
B) Liquid media for bacterial growth
C) Region on a bacterial operon
D) Matrix used for vertical get electrophoresis
_____ 6) What part of an amino acid differs from one to the other?
A) Amino acid
B) Carboxyl group
C) R group
D) All of the above
_____ 7) How can colorless proteins be monitored during electrophoresis?
A) Coomassie blue stain
B) Silver stain
C) PAGE loading dye
D) Ethidium bromide
_____ 8) Which of the following is a codon?
A) ATA
B) UAU
C) Tyrosine
D) All of the above
_____ 9) What is the purpose for SDS during electrophoresis?
A) Stain the proteins for visualization
B) Use as a loading dye
C) Denature the proteins and give them a negative charge
D) Conduct electricity
_____ 10) Which of the following is an example of a product easily found in nature?
A) Antibiotic
B) GMO
C) Recombinant DNA
D) All of the above
_____ 11) What are the most common molecules in cells?
A) Sugars
B) Lipids
C) Proteins
D) Nucleic acids
_____ 12) Glucose is a
A) peptide.
B) polysaccharide.
C) disaccharide.
D) monosaccharide
_____ 13)
A)
B)
C)
D)
Where does translation occur in prokaryotes?
In the endoplasmic reticulum
At the ribosomes
Outside the cell
In the nucleus
Please match the word with the description.
Activity assays
Concentration assays
Molarity
Buffer
pH
Biotechnology
Translation
ELISA
Genetic engineering
PK/PD assays
Transcription
Plasmids
Spectrophotometry
HPLC
Acid
Assay
Recombinant DNA
Bacteria cells
Potency assays
Gene therapy
Genetic disorders
Gel electrophoresis
Chromatography
14) A process of making an RNA code from DNA
_____________________________________
15) A process in which RNA is decoded and polypeptides are formed
_____________________________________
16) Extra small rings present in some bacteria
_____________________________________
17) Modifications of DNA code of an organism
_____________________________________
18) Parkinson's, diabetes, cystic and fibrosis (CF) are examples of
_____________________________________
19) Do not contain nucleus or membrane bound organelle So, DNA is floating in
cytoplasm
_____________________________________
20) Helps researchers measure the amount of protein present
_____________________________________
21) Determines the amount and length of activity for a given protein in a test organism
_____________________________________
22) Shows if a specific protein is present and conducting the expected reaction
_____________________________________
23) A highly specific test to determine protein concentration based on a particular a
antigen-antibody interaction
_____________________________________
24) Used for understanding the relationship between dosage and activity of a given
pharmaceutical
_____________________________________
25) A test for determining the presence or a particular characteristic of a protein of
interest
_____________________________________
26) An aqueous solution that ionizes and yields hydrogen ion
_____________________________________
27) A solution that resists changes in pH
_____________________________________
28) A measurement of the concentration of hydrogen ion in a sample
_____________________________________
29) Separation of molecules on or through a stationary phase
_____________________________________
30) Quantitative study of electromagnetic spectra deals with visible light, nearultraviolet, and near-infrared light sources
_____________________________________
31) Form of liquid chromatography used to separate compounds that are dissolved in
solution. Instruments consist of a reservoir of mobile phase, a pump, an injector, a
separation column, and a detector
_____________________________________
32) Defined as the moles of solute per liter of solution
_____________________________________
33) Using electricity to separate molecules on a gel slab
_____________________________________
34) Process of correcting DNA codes that cause genetic diseases and disorders
_____________________________________
35) The study and manipulation of living things or their components
_____________________________________
36) A combination of DNA from different sources in one molecule
_____________________________________
Answer the following questions.
37) A piece of one strand of a DNA molecule has the following code on it
ATG CCC GTG TTA AAA TGT GGG ATC CCC GGT GTG CCC TTA
What is the nitrogen base code on the opposite DNA strand?
38) A piece of structural gene has the code TAC CCC ATG GGG TAA GGC GTC
What is the mRNA transcript made?
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