Pathology Institute
‫המעבדה לפתולוגיה‬
Dr. Avi Eisenthal Ph.D.
Ph.D. ‫ד"ר אבי איזנטל‬
In vitro and in vivo models for studing immunotherapy
1. Generation of cytotoxic lymphocytes in culture by cytokines including, activated
natural killer cells from splenocytes and tumor infiltrating lymphocytes expanded
from the tumors after growing in mice.
2. Measurment of cytotoxic activity of cells in-vitro using
51
chromium-labeled tumor
targets (chromium release assay).
3. Measurement of efficacy of monoclonal antibodies in mediating antibody-dependent
cellular cytotoxicity (ADCC) in culture.
4. Effect of various anti-metabolites on the lymphocytes activity in-vitro.
5. Immunotherapy models in mice on established lung metastases.
References :
1. Eisenthal A. , R.B. Cameron, I. Uppenkamp , S.A. Rosenberg,
Effect of combined therapy with lymphokine-activated killer cells,
interleukin-2 and specific monoclonal antibody on established B16
melanoma lung metastases. Cancer Research, Vol. 48, pp. 71407145, 1988.
2. Eisenthal A. , S.A. Rosenberg , The effect of various cytokines
on the in vitro induction of ADCC in murine cells : Enhancement of
the IL-2 induced ADCC activity by IL-1. J. Immunology, Vol. 142, pp.
2307- 2313, 1989.
3. Eisenthal A, K. Eytan, E. Brazowski, B. Katz , I.
Shirazi, Y. Skornick. Effects of 5-fluorouracil on human
mitogen-activated peripheral blood lymphocytes from healthy
individuals. Anticancer Research Vol. 28:3785-3792, 2008
For further information
Pathology Institute
Tel Aviv Sourasky Medical Center, Tel Aviv University
6 Weizmann St, 64239, ISRAEL
Tel: +972-3-6974645
email: eisenthal@tasmc.health.gov.il