Notes – Cells and Cell Transport Organelle Nucleus Ribosomes Endoplasmic Reticulum Golgi Apparatus Lysosomes Vacuoles Mitochondria Chloroplast Cell Membrane Cell Wall Function Control Center Make Proteins Proteins (Rough ER), Smooth- full of enzymes HWY transports UPS of cell Modifies, sorts, packages Clean up – full of enzymes Storage Tank Powerhouse ATP Cellular Respiration Photosynthesis Homeostasis – allows things to move in and out of cell Protection and Support Passive Transport Diffusion, osmosis, facilitated diffusion NO Energy High to Low Plant/Animal Both Both Both Pro/Eukaryote Eukaryote Both Eukaryotes Both Eukaryotes Mostly animals Eukaryotes Large in Plants, many small vacuoles in animals Both Plants DO have Mitochondria! Eukaryotes PLANTS only (Autotrophs) Both Eukaryotes Plants Both Eukaryotes Both Active Transport Endocytosis, Exocytosis, Phagocytosis, PUMP ENERGY - ATP Low to High --Passive transport does NOT require energy (ATP); moves from an area of high concentration to an area of low concentration (“downhill”-down the concentration gradient) --3 types of passive transport diffusion>>particles move from higher to lower concentrations osmosis>>water moves from higher to lower concentrations across a membrane. facilitated diffusion>>particles from higher to lower concentrations using a channel/carrier protein --Active transport does require energy (ATP); moves from an area of low concentration to an area of high concentration (“uphill”-against the concentration gradient) --3 types of active transport: endocytosis>>moving particles into the cell using energy exocytosis>>moving particles out of the cell using energy protein pumps>> moving particles through a pump into or out through the cell membrane If the solution is… -Isotonic = the cell is balanced (homeostasis); Water can move in and out of the cell at the same rate= NO NET MOVEMENT Isotonic – SAME, Water will move in and out at SAME rate -Hypotonic = there is more water and less solute outside of the cell than inside the cell; water moves into the cell; the cell will swell/burst (in plants turgor pressure increases, and the cell becomes turgid) HypOtonic – LOW concentration in relation to cell, swell up like a HIPPO (water must move in to make it swell) -Hypertonic = there is less water and more solute outside the cell than inside the cell; water moves out of the cell; the cell will shrivel and wilt. ---Hypertonic – HIGH concentration in relation to cell, shrink (water must move out to make it shrink) Photosynthesis/Cellular Respiration Photosynthesis Cellular Respiration Function Energy storage Energy release Location Chloroplast Mitochondria Reactants CO₂ and H₂O and light C₆H₁₂O₆ and O₂ Products C₆H₁₂O₆ and O₂ CO₂ and H₂O Equation 6CO₂+ 6H₂O →C₆H₁₂O₆ + 6O₂ 6O₂ + C₆H₁₂O₆ →CO₂ + H₂O CO2 is a product of cellular respiration and burning of fossil fuels O2 is a product of Photosynthesis Photosynthesis - Chloroplast Cellular Respiration - Mitochondria Most ATP comes from ETC Aerobic – WITH Oxygen Anaerobic – WITHOUT Oxygen – FERMENTATION – Alcoholic and Lactic Acid Lactic Acid causes burning and pain in muscles because of build-up of lactic acid because there is not enough Oxygen present to keep up with demand. C6H12O6 + O2 yields 6CO2 + 6H20 + ATP Reactants yield Products Electron Transport Chain makes the most ATP!!! ATP – energy cell uses to carry out cell processes. ADP + P = stores energy ATP – P = releases energy CELL CYLCE and MITOSIS MITOSIS PMAT Prophase – Chromosomes appear Metaphase – Chromosomes Line up in MIDDLE Anaphase – Chromosomes move Apart or AWAY Telophase – TWO distinct nuclear envelopes Cytokinesis – cell division – animals form cleavage furrow. Plates form cell plate Mitosis 2 IDENTICAL Daughter Cells Body Cells (Somatic) 2N – Diploid - Human 2 (23) = 46 PMAT – Mitosis Meiosis 4 Genetically DIFFERENT Sex Cells – Egg (1/3 polar), 4 sperm 1N Haploid – Human 1 (23) PMAT x 2 Crossing over during Prophase I Mendel Genetics Mendel's Experiments Mendel chose pea plants as his experimental subjects, mainly because they were easy to cross and showed a variety of contrasting traits (purple vs white flowers, tall vs short stems, round vs wrinkled seeds) . Mendel chose true-breeding lines of each plant/trait he studied (true breeding lines always produced offspring of the same type) 2. He crossed a true breeding plant with a plant of the opposite trait (purple x white). He called this the Parental (P) generation. 3. He recorded data on the offspring of this cross (First Filial, F1) 4. He self pollinated the F1 offspring 5. He recorded data on the offspring of the second generation, calling it the Second Filial generation (F2) Analysis: The F1 generation always displayed one trait (he later called this the dominant trait) The F1 generation must have within it the trait from the original parents the white trait The F2 generation displayed the hidden trait, 1/4 of the F2 generation had it (he later called this hidden trait the recessive trait) Each individual has two "factors" that determine what external appearance the offspring will have. (We now call these factors genes or alleles) Mendel established three principles (or Laws) from his research 1. The Principle of Dominance and Recessiveness - one trait is masked or covered up by another trait 2. Principle of Segregation - the two factors (alleles) for a trait separate during gamete formation 3. Principle of Independent Assortment - factors of a trait separate independently of one another during gamete formation; another way to look at this is, whether a flower is purple has nothing to do with the length of the plants stems - each trait is independently inherited Mendel's factors are now called ALLELES. For every trait a person have, two alleles determine how that trait is expressed. We use letters to denote alleles, since every gene has two alleles, all genes can be represented by a pair of letters. PP = purple, Pp = purple, pp = white Homozogyous: when the alleles are the same, the individual is said to be homozygous, or true breeding. Letters designating a homozgyous individual could be capital or lowercase, as long as they are the same. Ex. AA, bb, EE, dd Heterozygous: when the alleles are different, in this case the DOMINANT allele is expressed. Ex. Pp, Aa Monohybrid cross = a cross involving one pair of contrasting traits. Ex. Pp x Pp Punnet Square: used to determine the PROBABILITY of having a certain type of offspring given the alleles of the parents Genotype: letters used to denote alleles (BB, Pp, etc) Phenotype: what an organism looks like (brown, purple) Examples of Punnet Squares: Crossing true-breeding parents or homozygous black and white. BB = Black, bb = white 1st example : Genotype of offspring = 0:2:0 BB:Bb:bb Phenotype = 4 Black:0 White 2nd example Crossing heterozygous Dominant with homozygous recessive Genotype of offspring = 0:2:2 Phenotype = 2 Black: 2 White