V.I.Pivtorak, O.A.Smiyukha, M.P.Bul’ko
Electron microscopic changes in the structural components of testicle after varicocele
modeling and surgery by the own method
Studding characteristics of veins and testicles parenchyma that are relevant to the emergence of a
varicocele and spermatogenesis disorders is still actual due to the fact that varicose veins causes
reproductive disorders and is one of the leading factors of male infertility.
Objective: To establish submicroscopic changes of testicles structural components after surgery
by the own method, performed in 30 days after varicocele modeling.
Experimental studies conducted on ten mongrel male dogs, weighing 9 to 12 kg. The dogs were
divided into control and experimental groups. The animals of the experimental group (five dogs)
were created a model of varicocele. Parenteral injected Gonadotropinum of 300 IU / kg and 0.2
ml of 1% Progesteronum solution daily for 10-days. After 10 days the midline laparotomy was
performed, and applied a ligature to the left renal vein at 2/3 of its diameter at the point between
the inferior vena and testicular veins. The bougie insertted through the renal vein damaged
valves of testicular vein. The wound was sutured up in layers. In 30 days after creation of
varicocele model the animals of experimental group were performed surgery by the own method.
Formed intervenous anastomoses to improve blood outflow of from the pampiniform plexus.
Retroperitoneal found testicular vein and deep vein encircling the ilium bone, crossed them so
that the proximal ending of the deep vein branch encircling the ilium bone, had a complete valve.
Using precision technique the renal ending of testicular vein and the proximal ending of the deep
vein encircling the ilium bone were sutured up together, forming anastomosis of "end-to-end"
type. Testicular ending of the testicular vein and the proximal ending supplementary superficial
vein (or superficial vein encircling the ilium bone) also sutured up together, forming anastomosis
of "end-to-end" type". Ligated distal endings of deep vein encircling the ilium bone, and
supplementary superficial vein (or superficial vein encircling the ilium bone). Examed the both
testicles in 60 days after creation of varicocele model.
Submicroscopic study showed that at varicocele there were discovered normalization of wall
structure and spermatogenic epithelium in the testicles of animals in many segments of the
convoluted seminiferous tubules under developed surgery (the own developed method). As part
of the tubular wall the myoid cells have the oblong shape nucleus without karyolemma
invaginations, preserved cytoplasm with myofibrils. Basement membrane clearly shaped,
moderate thickness. Many supported cells with large rounded nucleus containing euchromatin in
karyoplazm. There are small mitochondrias, mostly primary lysosomes, phagosomes in
cytoplasm. Saved the structure of spermatogonia, spermatocyte 1st and 2nd types. In spermatids
clear nucleus and formed acrosome. However, there are some dilated intercellular spaces and
some vacuoles in the cytoplasm of spermatocytes. Near the lumens of seminiferous tubules are
sperm cells that have constant ultrastructural organization. In the formed head clearly defined
oval osmiofilic nucleus, acrosome, a small cytoplasm lumbus. Behind the nucleus there are
proximal centriole and evidences of tail formation. Leydig cells are found often in loose
connective tissue that separates the convoluted seminiferous tubules. Ultrastructural organization
of their part indicates its active state.
Conclusions. After surgical correction of experimental varicocele using the own merhod there
was a high regenerative ability of testicles and the full restoration of its structural components.
Using of venous anastomoses helps to reduce venous pressure in the venous plexus, flowing
blood from the testicles, normalizing testicular hemodynamics and eliminating circulatory
hypoxia of testicles, stimulating venous drainage of the body, reducing the volume of its
bloodstream and improving the indices of spermatogenesis.