Canine XSCID exon 1 with underlined deletion present in the canine XSCID model:
ATGTTGAAGCCACCATTGCCACTCAGATCCCTCTTATTCCTGCAGCTGTCTCTGCTGGGGGTGGGGCT
GAACTCCACGGTCCCCATGCCCAATGGGAATGAAGACATCACACCTG
I realize this is only 100 base pairs, but if you can target within this somehow, that would be by far the best.
Below is a longer sequence of canine genomic DNA, which includes about 200 base pairs of additional
Upstream sequence, and 400 or so of downstream sequence. Note deletion is underlined, so these base pairs will
not be present in the gene which needs to be cut.
CGGAAACTATGACAGAAGGAAATGTGTGGGTGGGGAGGGGTAATGGGTGAGGGGCCCAGGTTCCT
GACAGTCTACACCCAGGGAACGAAGAGCAAGCGCCATGTTGAAGCCACCATTGCCACTCAGATCCC
TCTTATTCCTGCAGCTGTCTCTGCTGGGGGTGGGGCTGAACTCCACGGTCCCCATGCCCAATGGGAA
TGAAGACATCACACCTGGTGGGAAACATGGGACTGGAAGGGGTTGGTGAGAGGGGAGCCTGTGGG
AAGGGGTCGCATAGAAATCTTGAACCTGCCATGGGGCATTAGAAGGATGTGGGCAGAGTTTAAGAG
TGCTGTGGAGAAGGAAGGGGGAAGGGGTTCACTGCCTCATGCTGCTGCTTCTTCTGACCATCATTTC
TGGTTTTTCCCTCCCCCTCTTCATTTTCTCCACAATCTAGATTTCTTCCTGACCGCTACACCCTCCGAG
ACCCTCAGTGTTTCCTCCCTGCCCCTCCCAGAGGTCCAGTGTTTTGTGTTCAATGTTGAGTACATGAA
TTGCACTTGGAACAGCAGCTCTGAGCCCCGGCCCACCAACCTGACCCTGCACTACTG
Murine XID - exon 2 contains the mutation:
Exon 2 is:
TACACAAACAAGTTCCAGAGAGAGGAAGCCATGGCTGCAGTGATACTGGAGAGCATCTTTCTGAAG
CGCTCCCAGCAGAAAAAGAAAACATCACCTTTAAACTTCAAGAAGCGCCTGTTTCTCTTGACTGTAC
ACAAACTTTCATACTATGAATATGACTTTGAACGTGGG
The above exon with surrounding sequence is:
GTGTAAAGTGGTGTCTAGACATTACTGACCACCCAGGGCTATGTTGTCGGGCTCTAGAGCATTCAGT
TCCCTTACCCAAGAAGCCCAGTTTTGTAAACTCACCATGTAGAATCAATCCCCTGGTTAAATAGTAT
GAGTTTGGGCTGGAGAGAAGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCACAGGTCCTGAGTTC
AATTCCCAGCAACCACATGGTGGCTCACAGCCATCTGTAAAGGGATCCGATGCCCTCTTGCGGCATG
CAGGTGTACATGCAGATAGAGCACTCACACATAAAATAAATAAATCTTTTTAAAAAAAATAGTATG
CGTTCACTGTACTTGAAATTTGACCAAGATGGGGGGACTGTGGAAGAAGGAGCTCAGGCAGAAAGA
AACAAGATTCCCTGCTAGCGTTGTATATTTGTGTTTTGTTTACAACAGGGAGCAGGGCTGAGGCAGA
ACCAGGTAGGACAAAGATGACTATGCTAGAACCTTAAAAACTTTCCTTATCCACAGTACACAAACA
AGTTCCAGAGAGAGGAAGCCATGGCTGCAGTGATACTGGAGAGCATCTTTCTGAAGCGCTCCCAGC
AGAAAAAGAAAACATCACCTTTAAACTTCAAGAAGCGCCTGTTTCTCTTGACTGTACACAAACTTTC
ATACTATGAATATGACTTTGAACGTGGGGTAAGTTTCCCAAATGAGAAGTCCAAATTTCACCATAGT
CTTGGATCTTCTGTGAGAAATGAGATATGTGGTAGGTAGGAACTTAGTAGAGCTAAGTACATCAAAC
TTGAAAACATATTTCCTAGCCTAGGAGTAGGCACAGTCATGGCAGGGGAAAAGTCAAATATGTTAA
GAAGCTGTTCAGAATGTCTTACCATGGAATTGTGTGGTTAGTAAGAACTCACTCTATGCTTCTTGAG
AATGAAATCCAAGAATTTGACATTGCGTTTTTATTTCTGGAACAATAATGCATATAATCATTTATTCT
TCTATGTTATCTGCTTCCCATTTGGCATTTAAAGGTTAAATAAAATGCTGGATTTGTTTAAAACTAAC
AGGTTCTGCCAGGCACAGTGGCACATACTTTTAGTCCCGGCACTCAGGTTTACACAGTGAGTTCTAG
GCCAGCCAGGGCTACATTGTGAGACCTTGAGTCA
The mutation is an R28C, with CGC in the following sequence changed to TGC:
GAAGCGCCTG to:
GAAGTGCCTG
Canine PK deficiency:
Below are intron 4-exon5-intron5 of canine PK, including the deletion mutation underlined and bold.
GTTTGGGGCGGGGCCAAGGGGAGGCTGGCGAGCTAAAGCTTGCCGGGTTGAGAGGTGGGGGGCCG
GGCAACCTGCTCCTGAAATCCCCTCTCGCTGGTCCCCAGTACCATGCTCAGTCCATCGCCAACATCC
GGGAGGCCGTGGAGAGCTTTGCAACTTCTCCGCTCGGCTATCGCCCCGTGGCCATTGCCCTGGACAC
CAAGGGACCGGAGATACGGACCGGGGTCCTGAAGGGGGTGAGAGGCCAACTACGCCCGGGGGCTT
CCCTAGGCCCCCACCACCCTGTCAGGGACTGAGTCGCGGGTGGGGGCGGGGCCTGGGAGGGCAGGG
CAAGAGGCGGGTCTCAAGGCCCCTCTGGCTGGTCCCTAGG
FAH murine model in Grompe Pilot project:
mutant mouse ideal target sequence:
GCTTTCTTCGTAGGCCCTGGGAACAGATTCGGAGAGCCAATCCCCATTTCCAAAGCCCATGAACACA
TTTTCGGGATGGTCCTCATGAACGACTGGAGCAGTAATGCCTGGTGGCCCAGCTTCCTCTGATGTTCT
GTTCTTAGGGGCACACACAGGAGTTGGGTATGGGACAGGAGGCCTAAGTACTACAGGGGTGATACC
ATGC
Note underlined/bold A: In the WT mouse this is a G, and it is the terminal codon of exon 8. In the FAH5961SB
mouse model of tyrosinemia, this is an A, as above.
Markus' strategy utilizes a 4500 base pair region of homology delivered by an AAV vector, so it may be that it
would be interesting to target farther away, and look at the repair efficiency. Therefore, while it is best to try to
get something in the above sequence within 100 base pairs or so of the target, if you can find an easy target in the
following longer sequence, that would be worth going after as well.
(sequence from Andy – 3150 bp – genomic w/ intron)
AGACATGGAGTTGGAAATGGTGAGTTCTGTGTGGAATTTTGTTGAATGGGATCTACAAAGCGCTGTGGCAGTAAAGCTCT
CTTTCTGGGGCGGCACTTCACACCTGTTTGGCCACAAATAGCAGCCCATGTCCCCATGTGACCACTGAATGCAGTTGATGT
TCTTTCTTCAGATTCTGTGTTTGTGAATTCTCCCACTTGCTGAAACTTATTTGCAACCCAAATATTAACACAAGTCATTGCC
ACACAGAGAAGTGGAAAATTTGAGTCTCCTGATACACACATTTGTCCCCAGTTGAGGTCACACCAGGCTCCCTGTCTTCGT
GTTTCAGGTCTCATGCTATAAAAACATGCTATATTTTTGGTTTTTTTTCTCCACATATTTTCCACATTTGAACTACTTTTATT
ATTTACAATGCCTCCCAAGTGGAGGTCCATTGTGGTTCCCTAACTGCAGGAGGGCAATTCTAACAAGTGTTAGAAAAGCC
TCATTCATACCTAAGTTGTAGCTGTTGTTGGGGAATACAGTGTTGTTGAGCCAACACTATGTCAGCTATCTTTAACAGAAA
GAGGTAAAATATGGTGAGTGTTGTAGCCAGAGACTATGAAAACCCAGCTTGTTGCATCTTGTGGGAGCACCGGTCCACCT
TTTTTCTGTCACATTCATGGTGATTTTATACAATGTCACATGAACAGCAAGCATCAAGGCCCGGTGGCAGGGCCTCTATCA
CTTTAGAAAGACCCACACTAAGGCTCCACTCTTTATTTAAATTAAGCACTTTATTTTGAGAGTGCTGAAGACTCATATGTA
GTTGTGAGAAATGAGGTGGAGATCCCCTGAATTTGAAAGAGATGGGGTCCTTCATGCCTCGGTGCCCTCAGCTCTCTCTCT
CTCTCTCTCTCTCTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
ATCTTGAAGATTTTGCACTGTTTAAACCCTATCTCAAGGACAAATAAAACAGTGACCCTAGCCATGAACGTAGATGATTCT
GTACTGCTCAAGGAATTCCTTCTCCCCCGCACTTAGTTTCCCTTAGACATGATTGCTAGATGTTCTCTTGGTCCATGGCTAT
TGGACAGATGCCATTCCTTTTGGGATAGGTAGGGCTGTGAAGTCAACTTGTCAATCCTCCTGGAATGAGCACCCCTGATGT
TTTCATTCTTTACAAGTGTCATATACTGTCAGATGTGAGGGTATGGAGCCTGGAATACAGAGGAAGAATCAGAAAGGCCT
GGGGTGCCCTGGGGCTAGGGGGGAAAGGGCTCATGTGAGTAGACTTCCCACCTTAGAGACCCAGGGAAGTAATGCCAGG
TCCTCAGGCAGCCCTAGTCCCTGGTTGAACTTTGAAAATATTTTCCCTTTGCTCTGTAAGCCACAGTGACCCAGAGCATCG
GGTCATCTAGATTCTTACCAACTTTCTCCATGGCAGGCTTTCTTCGTAGGCCCTGGGAACAGATTCGGAGAGCCAATCCCC
ATTTCCAAAGCCCATGAACACATTTTCGGGATGGTCCTCATGAACGACTGGAGCGGTAATGCCTGGTGGCCCAGCTTCCTC
TGATGTTCTGTTCTTAGGGGCACACACAGGAGTTGGGTATGGGACAGGAGGCCTAAGTACTACAGGGGTGATACCATGCA
GACTTCTGACTCTGTGGGTGTGGGGCAGTCACAGCTTCCCTGAGTAGCTTTCTCATAAGTGGAAGGATGGAGCTGACAGA
ACCTAAAGCTTTATCAAGCCCTACACACTCCACTCACTGTTGCCAGCACCATCAGCTGCTGATGAAGAGCTGTACATGGA
GGGATTTTAGTCAGTGGCTGAATTGAGCCTTACTATTCATCAGCCCAGCATCTGAGTCCCAGCTCTACACCCTGATACCAT
GTGTAAGAGGAAGTGGGTCCAGAAATCTCAGGAGAGGGCCTTCCCCCATTCAGGCCCTTGGTAGGGACTGTCCTGTCATC
CTTCATTGTTCCCTAGCACAGCACCTCCAAGGACCTCTGTCTTCATACTGAATACCTTTCTGTTTGTTCCCTGGGAGATCTG
GTTGGCCAGAGCCATGTAGAGAGGTCCATGTAGGTCAAGGACAGGAAGCAGCTTGGAGTGAGTCTGTAAGCCACGGTGA
GACCTAAGCTTGCTGTCTTTTCATAGCACGAGACATCCAGCAATGGGAGTACGTCCCACTTGGGCCATTCCTGGGGAAAA
GCTTTGGAACCACAATCTCCCCGTGGGTGGTGCCTATGGATGCCCTCATGCCCTTTGTGGTGCCAAACCCAAAGCAGGTA
AGCACCCTTCTGCTGAAAACTCTGCTCTAAGTTCCGGCTTCCGCCCTTTCCTGTTGCCCTCTGATACTGAGACATGGTGATG
CTTGGTGGATCCTGCATTTTGTTTTGTGCAATGGCTCCCTCTAGAGTCTAATTTGCAGAATGCATTTTGGAATAGAGGCTAT
AAGGGAAAAGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCCAACAGGGTTCCCAGTGCAATA
TCCCACCCCTTCATTACAGTCACCCAGCTCTTATGGCCAATGCCTTGAAGGCCCTAACTTCTCTCAAACACGGCTCTGGTA
AATGACCTATCTGGTCCTCCCACATCTCTGGGTCTAAATCTCCAGAGGGCTCTGCTCTGCTCCTGGCTCCCTGCCTTCCCTC
CCCACACTCTCCCTGGCATTGTCTCACCAGCTGTGTACTGCTGCACTTTCTCCCTCACCCATCCATGTCATTTTATGCATGA
ACACTCTTGGGACTTTCCCCTGCAAATTGGTTCTTCATAAAAGTCCCTGCAGTTCCATTCTGTCTCCTTGGATCTTCCCCAA
CACTTCCCACCACAGCACCCACGTCTCCCCTCTGGTGGTCTCCCTCCTCTTTGTCTCTGTCCCTCCACTGTCCTTCTCATCTT
CTGGCCACCCAGGACCTGTTGGGCTCCTGCTTTAATGGTCCCCATCCTCCTTCACAGTCCTTCGCCCAGCTCTATCTTCACA
GAGATGATTGGATGCTGACTGCCTCCATCCTTCCCAAGGACTGTTTAAGTACAGCTGTCACCATTCCTGC
(Sequence from Barry – 2750bp – WT, unsure of origin)
AGACATGGAGTTGGAAATGGTGAGTTCTGTGTGGAATTTTGTTGAATGGGATCTACAAAGCGCTGTGGCAGTAAAGCTCT
CTTTCTGGGGCGGCACTTCACACCTGTTTGGCCACAAATAGCAGCCCATGTCCCCATGTGACCACTGAATGCAGTTGATGT
TCTTTCTTCAGATTCTGTGTTTGTGAATTCTCCCACTTGCTGAAACTTATTTGCAACCCAAATATTAACACAAGTCATTGCC
ACACAGAGAAGTGGAAAATTTGAGTCTCCTGATACACACATTTGTCCCCAGTTGAGGTCACACCAGGCTCCCTGTCTTCGT
GTTTCAGGTCTCATGCTATAAAAACATGCTATATTTTTGGTTTTTTTTCTCCACATATTTTCCACATTTGAACTACTTTTATT
ATTTACAATGCCTCCCAAGTGGAGGTCCATTGTGGTTCCCTAACTGCAGGAGGGCAATTCTAACAAGTGTTAGAAAAGCC
TCATTCATACCTAAGTTGTAGCTGTTGTTGGGGAATACAGTGTTGTTGAGCCAACACTATGTCAGCTATCTTTAACAGAAA
GAGGTAAAATATGGTGAGTGTTGTAGCCAGAGACTATGAAAACCCAGCTTGTTGCATCTTGTGGGAGCACCGGTCCACCT
TTTTTCTGTCACATTCATGGTGATTTTATACAATGTCACATGAACAGCAAGCATCAAGGCCCGGTGGCAGGGCCTCTATCA
CTTTAGAAAGACCCACACTAACCTCAGCTCTCTCTCTCTCTCTCTCTCTCTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTC
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATCTTGAAGATTTTGCACTGTTTAAACCCTATCTCAAGGACAAAT
AAAACAGTGACCCTAGCCATGAACGTAGATGATTCTGTACTGCTCAAGGAATTCCTTCTCCCCCGCACTTAGTTTCCCTTA
GACATGATTGCTAGATGTTCTCTTGGTCCATGGCTATTGGACAGATGCCATTCCTTTTGGGATAGGTAGGGCTGTGAAGTC
AACTTGTCAATCCTCCTGGAATGAGCACCCCTGATGTTTTCATTCTTTACAAGTGTCATATACTGTCAGATGTGAGGGTAT
GGAGCCTGGAATACAGAGGAAGAATCAGAAAGGCCTGGGGTGCCCTGGGGCTAGGGGGGAAAGGGCTCATGTGAGTAG
ACTTCCCACCTTAGAGACCCAGGGAAGTAATGCCAGGTCCTCAGGCAGCCCTAGTCCCTGGTTGAACTTTGAAAATATTTT
CCCTTTGCTCTGTAAGCCACAGTGACCCAGAGCATCGGGTCATCTAGATTCTTACCAACTTTCTCCATGGCAGGCTTTCTTC
GTAGGCCCTGGGAACAGATTCGGAGAGCCAATCCCCATTTCCAAAGCCCATGAACACATTTTCGGGATGGTCCTCATGAA
CGACTGGAGCGGTAATGCCTGGTGGCCCAGCTTCCTCTGATGTTCTGTTCTTAGGGGCACACACAGGAGTTGGGTATGGG
ACAGGAGGCCTAAGTACTACAGGGGTGATACCATGCAGACTTCTGACTCTGTGGGTGTGGGGCAGTCACAGCTTCCCTGA
GTAGCTTTCTCATAAGTGGAAGGATGGAGCTGACAGAACCTAAAGCTTTATCAAGCCCTACACACTCCACTCACTGTTGC
CAGCACCATCAGCTGCTGATGAAGAGCTGTACATGGAGGGATTTTAGTCAGTGGCTGAATTGAGCCTTACTATTCATCAG
CCCAGCATCTGAGTCCCAGCTCTACACCCTGATACCATGTGTAAGAGGAAGTGGGTCCAGAAATCTCAGGAGAGGGCCTT
CCCCCATTCAGGCCCTTGGTAGGGACTGTCCTGTCATCCTTCATTGTTCCCTAGCACAGCACCTCCAAGGACCTCTGTCTTC
ATACTGAATACCTTTCTGTTTGTTCCCTGGGAGATCTGGTTGGCCAGAGCCATGTAGAGAGGTCCATGTAGGTCAAGGAC
AGGAAGCAGCTTGGAGTGAGTCTGTAAGCCACGGTGAGACCTAAGCTTGCTGTCTTTTCATAGCACGAGACATCCAGCAA
TGGGAGTACGTCCCACTTGGGCCATTCCTGGGGAAAAGCTTTGGAACCACAATCTCCCCGTGGGTGGTGCCTATGGATGC
CCTCATGCCCTTTGTGGTGCCAAACCCAAAGCAGGTAAGCACCCTTCTGCTGAAAACTCTGCTCTAAGTTCCGGCTTCCGC
CCTTTCCTGTTGCCCTCTGATACTGAGACATGGTGATGCTTGGTGGATCCTGCATTTTGTTTTGTGCAATGGCTCCCTCTAG
AGTCTAATTTGCAGAATGCATTTTGGAATAGAGGCTATAAGGGAAAAGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
GTGTGTGTGTGTGTGTCCAACAGGGTTCCCAGTGCAATATCCCACCCCTTCATTACAGTCACCCAGCTCTTATGGCCAATG
CCTTGAAGGCCCTAACTTCTCTCAAACACGGCTCTGGTAAATGACCTATCTGGTCCTCCCACATCTCTGGGTCTAAATCTC
CAGAGGGCTCTGCTCTGCTCCTGGCTCCCTGCCTTCCCTCCCCACACTCTCCCTGGCATTGTCTCACCAGCTGTGTACTGCT
GCACTTTCTCCCTCACCCATCCATGTCATTTTATGCATGAACACTCTTGGGACTTTCCCCTGCAAATTGGTTCTTCATAAAA
Primate (Macacaque) CCR5:
We simply want to disrupt this gene, so can target anywhere.
> NAME = 'nemestrina promotor and UTR.fa' : TYPE = DNA
GCACAGGGTTAACCCGAAATCCAGGATCCCCCTCTACATTTAAAGTTGGTTTAAGTTGGCTTCAATTGATGGCAAC
TCTTAAGATAATCAGAATTTTCTTACCTTTTTAGCTTTACTGTTGAAAAGCCCTGTGATCTTGTACAAATCATTTG
CTTCTTGGGTAGTAATTTCTTTTACTCAAACGTGAGCTTTTGACTAGGTGAATGTAAATGTTCTTCTAGCTCTAAT
ATCCTTTATTCTTTATATTTTCTAACAGATTCTGTGTAGTTGGATGAGCAGAGAACAAAAACAAAATAATCCAGTG
AGGAAAGCCCGTGAATAAACTTTCAGACCCGAGATCTACTCTCTAGCCTATTTTAAGCTCAACTTAAAAAGAAGAA
CTGTTCTCTGATTCTTTTCGCCTTCAATACTCAATGATTTAACTCCGCCCTCCTTCAAAAGAAACAGCATTTCCTA
CTTTTATACTGTCTATATGATTGATTTGCACAACTCATCTGCCAGAAGAGCTGAGACATCCGTTCCCTTACAAGAA
ACTCTCCCCGGTAAGTAACCTCAGCTGCTTGGCCTATTAGTTAGCTTCTGAGATGAGTAAAAGCCTTAACAGGAAA
CCCATAGAAGACATTTGGCAAACACAAAGTGCTCATACAATTATCTTAAAATATAATCTTTAAGATGAGGAAAGGG
TCAGAGTTTAGAATGAGTTTCAGATTATTATAACATCAAAGATACAAAACATGATTGTGAGTGAAAGACTTTAAAG
GGAGCAATAGTATTTTAATAACTAACAGTCCTTACCTCTCAAAAGAAAGATTTGCAGAGAGATGAGTCTTAGCTGA
AATCTTGAAATCTTATCTTCTGCTAAGGAGAACTAAACCCTCTCCAGTGAAATGCCTTCTGAATATGTGCC
> NAME = 'nemestrina CCR5 mRNA.fa' : TYPE = DNA
ATGGACTATCAAGTGTCAAGTCCAACCTATGACATCGATTATTATACATCGGAACCCTGCCAAAAAATCAATGTGA
AACAAATCGCAGCCCGCCTCCTGCCTCCGCTCTACTCACTGGTGTTCATCTTTGGTTTTGTGGGCAACATACTGGT
CGTCCTCATCCTGATAAACTGCAAAAGGCTGAAAAGCATGACTGACATCTACCTGCTCAACCTGGCCATCTCTGAC
CTGCTTTTCCTTCTTACTGTCCCCTTCTGGGCTCACTATGCTGCTGCCCAGTGGGACTTTGGAAATACAATGTGTC
AACTCTTGACAGGGCTCTATTTTATAGGCTTCTTCTCTGGAATCTTCTTCATCATCCTCCTGACAATCGATAGGTA
CCTGGCTATCGTCCATGCTGTGTTTGCTTTAAAAGCCAGGACAGTCACCTTTGGGGTGGTGACAAGTGTGATCACT
TGGGTGGTGGCTGTGTTTGCCTCTCTCCCAGGAATCATCTTTACCAGATCTCAGAGAGAAGGTCTTCATTACACCT
GCAGCTCTCATTTTCCATACAGTCAGTATCAATTCTGGAAGAATTTTCAGACATTAAAGATGGTCATCTTGGGGCT
GGTCCTGCCGCTGCTTGTCATGGTCATCTGCTACTCGGGAATCCTGAAAACTCTGCTTCGGTGTCGAAACGAGAAG
AAGAGGCACAGGGCTGTGAGGCTTATCTTCACCATCATGATTGTTTATTTTCTCTTCTGGGCTCCCTACAACATTG
TCCTTCTCCTGAACACCTTCCAGGAATTCTTTGGCCTGAATAATTGCAGTAGCTCTAACAGGTTGGACCAAGCCAT
GCAGGTGACAGAGACTCTTGGGATGACACACTGCTGCATCAACCCCATCATCTATGCCTTTGTCGGGGAGAAGTTC
AGAAACTACCTCTTAGTCTTCTTCCAAAAGCACATTGCCAAACGCTTCTGCAAATGCTGTTCCATTTTCCAGCAAG
AGGCTCCCGAGCGAGCAAGTTCAGTTTACACCCGATCCACTGGGGAGCAGGAAATATCTGTGGGCTTGTGA
Anopheles CTLMA2:
I believe this is straight genomic sequence as it had the following info with it:
ref|NT_078265.1|:13663306-13664186 Anopheles gambiae str. PEST chromosome 2L (CDS 176..700)
GCCTTCTTCACGCATACCTCTTATCATCGCATCCCAATCGCAGTTGTACAGAAGGTGCTTCTACGATC
ATTCAAGCTCACTCTCAGAAAACGTATAGTCTTTAGAAGTTTGTGTGGTTTTCTGTACTGTTATACTG
TGTTCCTTTTTACAGAAAAAGTGGTAAACCATTTTTAAAATGAATTTAATGATCACAATTCTAATTGC
TGTCATCACAGTGGTTCGTGGTGACCTATCGGTTCCTAAAACTGTGGATGGTAAGATTTTTGGGGTTT
TTGTTATGGTCCAATTTAGGTTCGATTCACGTAATAATTTGTTATAACCATTTCCAGATATTCTTCAA
CAAAACCCATGCCTTTGCCCATGCAAACCGTTCGAGGAGAAAGAATACTTCATTCCCATCAGTCGGG
TAGAATAGAATTCCAAACCGTATCCAAATGCAACCTAAACCTTTATCACCCATTCCATCCTTCGCAG
ACGAGCGACTGGTTCGGTGCCGTTGCCTACTGCCACAGCGTTGGCATGGAGCTGGCCGAGGTGCTGA
ACGAAGACGAAGCACGAGCCATGGGCGAAGTGATCGCGGAGGAAGAATCCGACTCGGATGACGAG
TTTTACTGGATCGGAGCGAATGATCTCGGCGTGCAGGGTACTTACCGGTGGGCATTAGCTGGACGGC
CAGTGTTGTACAGCCAATGGGCTGCAGGTGAACCGAACCATGCTCGCGGTGAGAATGGCCAACAGC
CCGCCGAACGATGCGTCGCTGTTGCGATGGACAAGTACGAGTGGAACGATTTCCAATGTACGCAGC
AGAAGCCGTTCATCTGTCAACAGTTCCGAAAACATTGAAAAGTGTGAATAAATCGTGCGTTTTCTCG
GCAGAAACA
Murine ROSA26 genomic
This loci is a ubiquitiously expressed locus, identified in mice, that seems to be very easy to target. No one knows
exactly why. It would be a useful safe harbor site, if something looks designable for it. We would take targets for
either human or mouse, but if you can find something conserved between them sequence wise, that would be ideal
(as would anything also conserved in the canine genome).
TGGCCTCTCCGCCCACCGCCCCACACTTATTGGCCGGTGCGCCGCCAATCAGCGGAGGCTGCCGGGGCCGCCTAAAGAAGAGGCTGTGC
TTTGGGGCTCCGGCTCCTCAGAGAGCCTCGGCTAGGTAGGGGATCGGGACTCTGGCGGGAGGGCGGCTTGGTGCGTTTGCGGGGATGGG
CGGCCGCGGCAGGCCCTCCGAGCGTGGTGGAGCCGTTCTGTGAGACAGCCGGGTACGAGTCGTGACGCTGGAAGGGGCAAGCGGGTGG
TGGGCAGGAATGCGGTCCGCCCTGCAGCAACCGGAGGGGGAGGGAGAAGGGAGCGGAAAAGTCTCCACCGGACGCGGCCATGGCTCG
GGGGGGGGGGGGCAGCGGAGGAGCGCTTCCGGCCGACGTCTCGTCGCTGATTGGCTTCTTTTCCTCCCGCCGTGTGTGAAAACACAAAT
GGCGTGTTTTGGTTGGCGTAAGGCGCCTGTCAGTTAACGGCAGCCGGAGTGCGCAGCCGCCGGCAGCCTCGCTCTGCCCACTGGGTGGG
GCGGGAGGTAGGTGGGGTGAGGCGAGCTGGACGTGCGGGCGCGGTCGGCCTCTGGCGGGGCGGGGGAGGGGAGGGAGGGTCAGCGAA
AGTAGCTCGCGCGCGAGCGGCCGCCCACCCTCCCCTTCCTCTGGGGGAGTCGTTTTACCCGCCGCCGGCCGGGCCTCGTCGTCTGATTGG
CTCTCGGGGCCCAGAAAACTGGCCCTTGCCATTGGCTCGTGTTCGTGCAAGTTGAGTCCATCCGCCGGCCAGCGGGGGCGGCGAGGAGG
CGCTCCCAGGTTCCGGCCCTCCCCTCGGCCCCGCGCCGCAGAGTCTGGCCGCGCGCCCCTGCGCAACGTGGCAGGAAGCGCGCGCTGGG
GGCGGGGACGGGCAGTAGGGCTGAGCGGCTGCGGGGCGGGTGCAAGCACGTTTCCGACTTGAGTTGCCTCAAGAGGGGCGTGCTGAGC
CAGACCTCCATCGCGCACTCCGGGGAGTGGAGGGAAGGAGCGAGGGCTCAGTTGGGCTGTTTTGGAGGCAGGAAGCACTTGCTCTCCC
AAAGTCGCTCTGAGTTGTTATCAGTAAGGGAGCTGCAGTGGAGTAGGCGGGGAGAAGGCCGCACCCTTCTCCGGAGGGGGGAGGGGAG
TGTTGCAATACCTTTCTGGGAGTTCTCTGCTGCCTCCTGGCTTCTGAGGACCGCCCTGGGCCTGGGAGAATCCCTTCCCCCTCTTCCCTCG
TGATCTGCAACTCCAGTCTTTCTAGAAGATGGGCGGGAGTCTTCTGGGCAGGCTTAAAGGCTAACCTGGTGTGTGGGCGTTGTCCTGCA
GGGGAATTGAACAGGTGTAAAATTGGAGGGACAAGACTTCCCACAGATTTTCGGTTTTGTCGGGAAGTTTTTTAATAGGGGCAAATAAG
GAAAATGGGAGGATAGGTAGTCATCTGGGGTTTTATGCAGCAAAACTACAGGTTATTATTGCTTGTGATCCGCCTCGGAGTATTTTCCAT
CGAGGTAGATTAAAGACATGCTCACCCGAGTTTTATACTCTCCTGCTTGAGATCCTTACTACAGTATGAAATTACAGTGTCGCGAGTTAG
ACTATGTAAGCAGAATTTTAATCATTTTTAAAGAGCCCAGTACTTCATATCCATTTCTCCCGCTCCTTCTGCAGCCTTATCAAAAGGTATT
TTAGAACACTCATTTTAGCCCCATTTTCATTTATTATACTGGCTTATCCAACCCCTAGACAGAGCATTGGCATTTTCCCTTTCCTGATCTT
AGAAGTCTGATGACTCATGAAACCAGACAGATTAGTTACATACACCACAAATCGAGGCTGTAGCTGGGGCCTCAACACTGCAGTTCTTT
TATAACTCCTTAGTACACTTTTTGTTGATCCTTTGCCTTGATCCTTAATTTTCAGTGTCTATCACCTCTCCCGTCAGGTGGTGTTCCACATT
TGGGCCTATTCTCAGTCCAGGGAGTTTTACAACAATAGATGTATTGAGAATCCAACCTAAAGCTTAACTTTCCACTCCCATGAATGCCTC
TCTCCTTTTTCTCCATTTATAAACTGAGCTATTAACCATTAATGGTTTCCAGGTGGATGTCTCCTCCCCCAATATTACCTGATGTATCTTAC
ATATTGCCAGGCTGATATTTTAAGACATTAAAAGGTATATTTCATTATTGAGCCACATGGTATTGATTACTGCTTACTAAAATTTTGTCAT
TGTACACATCTGTAAAAGGTGGTTCCTTTTGGAATGCAAAGTTCAGGTGTTTGTTGTCTTTCCTGACCTAAGGTCTTGTGAGCTTGTATTT
TTTCTATTTAAGCAGTGCTTTCTCTTGGACTGGCTTGACTCATGGCATTCTACACGTTATTGCTGGTCTAAATGTGATTTTGCCAAGCTTCT
TCAGGACCTATAATTTTGCTTGACTTGTAGCCAAACACAAGTAAAATGATTAAGCAACAAATGTATTTGTGAAGCTTGGTTTTTAGGTTG
TTGTGTTGTGTGTGCTTGTGCTCTATAATAATACTATCCAGGGGCTGGAGAGGTGGCTCGGAGTTCAAGAGCACAGACTGCTCTTCCAGA
AGTCCTGAGTTCAATTCCCAGCAACCACATGGTGGCTCACAACCATCTGTAATGGGATCTGATGCCCTCTTCTGGTGTGTCTGAAGACCA
CAAGTGTATTCACATTAAATAAATAAATCCTCCTTCTTCTTCTTTTTTTTTTTTTTAAAGAGAATACTGTCTCCAGTAGAATTTACTGAAGT
AATGAAATACTTTGTGTTTGTTCCAATATGGTAGCCAATAATCAAATTACTCTTTAAGCACTGGAAATGTTACCAAGGAACTAATTTTTA
TTTGAAGTGTAACTGTGGACAGAGGAGCCATAACTGCAGACTTGTGGGATACAGAAGACCAATGCAGACTTTAATGTCTTTTCTCTTAC
ACTAAGCAATAAAGAAATAAAAATTGAACTTCTAGTATCCTATTTGTTTAAACTGCTAGCTTTACTTAACTTTTGTGCTTCATCTATACAA
AGCTGAAAGCTAAGTCTGCAGCCATTACTAAACATGAAAGCAAGTAATGATAATTTTGGATTTCAAAAATGTAGGGCCAGAGTTTAGCC
AGCCAGTGGTGGTGCTTGCCTTTATGCCTTTAATCCCAGCACTCTGGAGGCAGAGACAGGCAGATCTCTGAGTTTGAGCCCAGCCTGGTC
TACACATCAAGTTCTATCTAGGATAGCCAGGAATACACACAGAAACCCTGTTGGGGAGGGGGGCTCTGAGATTTCATAAAATTATAATT
GAAGCATTCCCTAATGAGCCACTATGGATGTGGCTAAATCCGTCTACCTTTCTGATGAGATTTGGGTATTATTTTTTCTGTCTCTGCTGTT
GGTTGGGTCTTTTGACACTGTGGGCTTTCTTTAAAGCCTCCTTCCTGCCATGTGGTCTCTTGTTTGCTACTAACTTCCCATGGCTTAAATG
GCATGGCTTTTTGCCTTCTAAGGGCAGCTGCTGAGATTTGCAGCCTGATTTCCAGGGTGGGGTTGGGAAATCTTTCAAACACTAAAATTG
TCCTTTAATTTTTTTTTTAAAAAATGGGTTATATAATAAACCTCATAAAATAGTTATGAGGAGTGAGGTGGACTAATATTAAATGAGTCC
CTCCCCTATAAAAGAGCTATTAAGGCTTTTTGTCTTATACTTAACTTTTTTTTTAAATGTGGTATCTTTAGAACCAAGGGTCTTAGAGTTT
TAGTATACAGAAACTGTTGCATCGCTTAATCAGATTTTCTAGTTTCAAATCCAGAGAATCCAAATTCTTCACAGCCAAAGTCAAATTAAG
AATTTCTGACTTTTAATGTTAATTTGCTTACTGTGAATATAAAAATGATAGCTTTTCCTGAGGCAGGGTCTCACTATGTATCTCTGCCTGA
TCTGCAACAAGATATGTAGACTAAAGTTCTGCCTGCTTTTGTCTCCTGAATACTAAGGTTAAAATGTAGTAATACTTTTGGAACTTGCAG
GTCAGATTCTTTTATAGGGGACACACTAAGGGAGCTTGGGTGATAGTTGGTAAAATGTGTTTCAAGTGATGAAAACTTGAATTATTATCA
CCGCAACCTACTTTTTAAAAAAAAAAGCCAGGCCTGTTAGAGCATGCTTAAGGGATCCCTAGGACTTGCTGAGCACACAAGAGTAGTTA
CTTGGCAGGCTCCTGGTGAGAGCATATTTCAAAAAACAAGGCAGACAACCAAGAAACTACAGTTAAGGTTACCTGTCTTTAAACCATCT
GCATATACACAGGGATATTAAAATATTCCAAATAATATTTCATTCAAGTTTTCCCCCATCAAATTGGGACATGGATTTCTCCGGTGAATA
GGCAGAGTTGGAAACTAAACAAATGTTGGTTTTGTGATTTGTGAAATTGTTTTCAAGTGATAGTTAAAGCCCATGAGATACAGAACAAA
GCTGCTATTTCGAGGTCTCTTGGTTTATACTCAGAAGCACTTCTTTGGGTTTCCCTGCACTATCCTGATCATGTGCTAGGCCTACCTTAGG
CTGATTGTTGTTCAAATAAACTTAAGTTTCCTGTCAGGTGATGTCATATGATTTCATATATCAAGGCAAAACATGTTATATATGTTAAAC
ATTTGTACTTAATGTGAAAGTTAGGTCTTTGTGGGTTTGATTTTTAATTTTCAAAACCTGAGCTAAATAAGTCATTTTTACATGTCTTACA
TTTGGTGGAATTGTATAATTGTGGTTTGCAGGCAAGACTCTCTGACCTAGTAACCCTACCTATAGAGCACTTTGCTGGGTCACAAGTCTA
GGAGTCAAGCATTTCACCTTGAAGTTGAGACGTTTTGTTAGTGTATACTAGTTTATATGTTGGAGGACATGTTTATCCAGAAGATATTCA
GGACTATTTTTGACTGGGCTAAGGAATTGATTCTGATTAGCACTGTTAGTGAGCATTGAGTGGCCTTTAGGCTTGAATTGGAGTCACTTG
TATATCTCAAATAATGCTGGCCTTTTTTAAAAAGCCCTTGTTCTTTATCACCCTGTTTTCTACATAATTTTTGTTCAAAGAAATACTTGTTT
GGATCTCCTTTTGACAACAATAGCATGTTTTCAAGCCATATTTTTTTTCCTTTTTTTTTTTTTTTTTGGTTTTTCGAGACAGGGTTTCTCTGT
ATAGCCCTGGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCCTGAGTGCCGGGAT
TAAAGGCGTGCACCACCACGCCTGGCTAAGTTGGATATTTTGTTATATAACTATAACCAATACTAACTCCACTGGGTGGATTTTTAATTC
AGTCAGTAGTCTTAAGTGGTCTTTATTGGCCCTTCATTAAAATCTACTGTTCACTCTAACAGAGGCTGTTGGTACTAGTGGCACTTAAGC
AACTTCCTACGGATATACTAGCAGATTAAGGGTCAGGGATAGAAACTAGTCTAGCGTTTTGTATACCTACCAGCTTTATACTACCTTGTT
CTGATAGAAATATTTCAGGACATCTAGAGTGTACTATAAGGTTGATGGTAAGCTTATAAGGAACTTGAAAGTGGAGTAACTACTCCATT
TCTCTGAGGGGAGAATTAAAATTTTTGACCAAGTGTTGTTGAGCCACTGAGAATGGTCTCAGAACATAACTTCTTAAGGAACCTTCCCA
GATTGCCCTCAACACTGCACCACATTTGGTCCTGCTTGAACATTGCCATGGCTCTTAAAGTCTTAATTAAGAATATTAATTGTGTAATTAT
TGTTTTTCCTCCTTTAGATCATTCCTTGAGGACAGGACAGTGCTTGTTTAAGGCTATATTTCTGCTGTCTGAGCAGCAACAGGTCTTCGAG
ATCAACATGATGTTCATAATCCCAAGATGTTGCCATTTATGTTCTCAGAAGCAAGCAGAGGCATGATGGTCAGTGACAGTAATGTCACT
GTGTTAAATGTTGCTATGCAGTTTGGATTTTTCTAATGTAGTGTAGGTAGAACATATGTGTTCTGTATGAATTAAACTCTTAAGTTACACC
TTGTATAATCCATGCAATGTGTTATGCAATTACCATTTTAAGTATTGTAGCTTTCTTTGTATGTGAGGATAAAGGTGTTTGTCATAAAATG
TTTTGAACATTTCCCCAAAGTTCCAAATTATAAAACCACAACGTTAGAACTTATTTATGAACAATGGTTGTAGTTTCATGCTTTTAAAAT
GCTTAATTATTCAATTAACACCGTTTGTGTTATAATATATATAAAACTGACATGTAGAAGTGTTTGTCCAGAACATTTCTTAAATGTATAC
TGTCTTTAGAGAGTTTAATATAGCATGTCTTTTGCAACATACTAACTTTTGTGTTGGTGCGAGCAATATTGTGTAGTCATTTTGAAAGGAG
TCATTTCAATGAGTGTCAGATTGTTTTGAATGTTATTGAACATTTTAAATGCAGACTTGTTCGTGTTTTAGAAAGCAAAACTGTCAGAAG
CTTTGAACTAGAAATTAAAAAGCTGAAGTATTTCAGAAGGGAAATAAGCTACTTGCTGTATTAGTTGAAGGAAAGTGTAATAGCTTAGA
AAATTTAAAACCATATAGTTGTCATTGCTGAATATCTGGCAGATGAAAAGAAATACTCAGTGGTTCTTTTGAGCAATATAACAGCTTGTT
ATATTAAAAATTTTCCCCACAGATATAAACTCTAATCTATAACTCATAAATGTTACAAATGGATGAAGCTTACAAATGTGGCTTGACTTG
TCACTGTGCTTGTTTTAGTTATGTGAAAGTTTGGCAATAAACCTATGTCCTAAATAGTCAAACTGTGGAATGACTTTTTAATCTATTGGTT
TGTCTAGAACAGTTATGTTGCCATTTGCCCTAATGGTGAAAGAAAAAGTGGGGAGTGCCTTGGC
Human ROSA26 genomic fragment partial
TGGCCTCTCCGCCCACCGCCCCGCACCCATTGGCCCACTCGCCGCCAATCAGCGGAAGCCGCCGGGGCCGCCTAGAGAAG
AGGCTGTGCTTCGGCGCTCCGGCTCCTCAGAGAGCCTCGGCTAGGTAGGGGAGCGGAACTCTGGTGGGAGGGGAGGTGC
GGTGCACTGGGGGGATGGGTGGCTAGGGGGGCCGTCTGGTGGCTTGCGGGGGTTGCCTTTCCCGTGGGAAGTCGGGAAC
ATAATGTTTGTTACGTTGGGAGGGAAAGGGGTGGCTGGATGCAGGCGGGAGGGAGGCCCGCCCTGCGGCAACCGGAGGG
GGAGGGAGAAGGGAGCGGAAAATGCTCGAAACCGGACGGAGCCATTGCTCTCGCAGAGGGAGGAGCGCTTCCGGCTAG
CCTCTTGTCGCCGATTGGCCGTTTCTCCTCCCGCCGTGTGTGAAAACACAAATGGCGTATTCTGGTTGGAGTAAAGCTCCT
GTCAGTTACACCGTCGGGAGTACGCAGCCGCTTAGCGACTCTCGCGTTGCCCCCTGGGTGGGGCGGGTAGGTAGGTGGGG
TGTAGAGATGCTGGGTGTGCGGGCGCGGCCGGCCTCCTGCGGCGGGAGGGGAGGGTCAGTGAAATTGGCTCTGGCGCGG
GCGTCCTCCCACCCTCCCCTTCCTTCGGGGGAGTCGGTTTACCCGCCGCCTGCTTGTCTTCGACACCTGATTGGCTGTCGAA
GCTGTGGGACCGGGCCCTTGCTACTGGCTCGAGTCTCACATGAGCGAAACCACTGCGCGGGGCGCGGGGGTGGCGGGGA
GGCGGGCGTTGGTACGGTCCTCCCCGAGGCCGAGCGCCGCAGTGTCTGGCCCCGCGCCCCTGCGCAACGTGGCAGGAAGC
GCGCGCTGGAGGCGGGGGCGGGCTGCCGGCCGAGACTTCTGGATGGCGGCGGCCGCGGCTCCGCCCCGGGTTCCCACCG
CCTGAAGGGCGAGACAAGCCCGACCTGCTACAGGCACTCGTGGGGGTGGGGGAGGAGCGGGGGTCGGTCCGGCTGGTTT
GTGGGTGGGAGGCGCTTGTTCTCCAAAAACCGGCGCGAGCTGCAATCCTGAGGGAGCTGCGGTGGAGGAGGTGGAGAGA
AGGCCGCACCCTTCTGGGCAGGGGGAGGGGAGTGCCGCAATACCTTTATGGGAGTTCTCTGCTGCCTCCCGTCTTGTAAG
GACCGCCCTGGGCCTGGAAGAAGCCCTCCCTCCTTTCCTCCTCGCGTGATCTCGTCATCGCCTCCATGTCGAGTCGCTTCTC
GATTATGGGCGGGATTCTTTTGCCTAGGCTTAAGGGGCTAACTTGGTCCCTGGGCGTTGCCCTGCAGGGGAGTGAGCAGC
TGTAAGATTTGAGGGGCGACTCCGATTAGTTTATCTTCCCACGGACTAGAGTTGGTGTCGAGGTTATTGTAATAAGGGTGG
GGTAGGGAAATGGAGCTTAGTCATTCACCTGGGGCTGATTTTATGCAACGAGACTGCGGATTATCACTACTTATCATTTTT
GGAGCATTTTTCTAGAGACAGACATAAAGCATGATCACCTGAGTTTTATAC
Canine ROSA26 aligned genomic fragment
TGGCCTCTCCGCCCACCGCCCCTCGCCCATTGGCCAGTTCGCCGCCAATCAGCGGAAGCCGCCGGGGCCTCCTAGAGAAG
AGGCTGTGCTCTGGGGCTCCGGCTCCTCAGAGAGCCTCGGCTAGGTAGGGGAGCGGGACTCTGGTGGGAGGGGGGGGTC
CGGTGGACGGGCGGGGAGACGGGTGGCTGAGGCGGTCCGACCGAGAGACGCGGGTCGCCTTTCCCCGCGGGAAGTCGGG
GGTGTAACGTTTGTTAGGCTGGAAGGGAAAGAGGTTGGCCAGAAGTAGGCAGGACCGTGGCCCGCCCTGCGGCAACCGG
AGGGGGAGGGAGGAGAGCGGAAAAGGCTTGAATCCGGACGGGGCCATTGCTCCTGCAGAAGGAGGAGCGCTTCCGGCTC
ACCTCTCGTCACTGATTGGCCGCTTCTCCTCCCGCCGTGTGTGAAAACACAAATGGCGTGTTTTGGTTGGAGTAAAGCGCC
TGTCAGTTACGGCCTCGGGAGTGCGCAGCCGCCTGGGGACTCTCGCATTGCTCCCTGGGTGGGATGGGTAGGTAGACGGG
GTGGGGAGAGAGCTGGAGGAGCGGGCGCGGCCGACCTCCCGCGGGGGGCGGGGGCCGGGGCCGGGGCCGGGGCCGGGG
GGCGGGGGGGTCAGTGAAAGTGCCTCCCGAGCCGGCGTCCCGCCGCCCGCCCCTTCCTCGCGGGGAGTAGGGTTACCCGC
CGCCCGTGTGGCTTCGGCACCTGATTGGCTGCTGAAGCCGGGGAACGGCCCCTTGCCATTGGCTCGGGTCCCAATTGAGC
GAAACCACTGAGCGGGTCAGCAGGGGGGGCGGGGGGGCGGGGAGGCGGTCGTTGGTACGGTCCTTCCCGAGGCCCCGCG
CCGCAGTGTCTGGCCCCGCGCCCCTGCGCAACGTGGCAGGAAGCGCGCGCTGGAGGCGGAGGCGGAGGCGGAGGCGGGC
TGCTGGGCCGAGGCTTCTGGGTGGCGGCGACCGCGGCTCCGCCCCGGGCGTCCGCCGCCTGAAGGACGAGACGAGCTGG
ATCCGCTCGCGGACTCGCGGGGGTGGGGGGAGGAGCGGGGGTCGGTCCTGCTGGTTTGTGGGAGGGAGGTGCGTGGTCT
CTGAAAATCGGCGCGAGCTGCAATCCTGAGGGATCTGCAGTGGAGGAGGCGGAGAGAAGGCCGCACCCTTCTCGGTAGG
GGGAGGGGAGTGCCGCAATACCTTTATGGGAGTTCTCGGCTGCCTCCTGTCTCCTAAGGACCGCCCTGGGCCTAGAAGAA
TCCCTCCCTCCCCCGCGATCTCGTCATCGCCTCCATGTCGAGTTGCTCCTTCTCGATTGTGGGCGGGACTCTCTGTGCCCAG
GCTTTACCTGGTGTCTGGGCGTTGTCCTGCAGGGGATTGAGCAGGTGTGAGATTTCTCTCTTCACCCTGCTTGAGTTTGTGT
CACAAAATAATTATAATAGGGGTGGAGGAGGGAAATGGGAGTCCAGGCATTCATCTCGGGCTGATTTTATGCAGCAAGG
CTGCGTGTTATTACTACTTAAGATCGTTTTTGTAGTGCTTTTCCTGTGGATAAAAGGAGGTTAAGGGCATGGTCATCTGAG
TTTTTTACTCACGTCGTTTGAGA
Additional targets
 Murine XSCID (Word from Andy is this doesn’t need additional site searches because of a natural target
and other sites for Sce targeting).