CAFT training in Veterinary Microbiology: Proposal for the year 2013-13. Department of Veterinary Microbiology,
LLRUVAS, Hisar-125004 (Haryana)
Proforma for submitting proposal for organization of CAFT Programmes (20122013)
1.
Topic of Training Programme:
‘Advances in diagnostics of infectious diseases of domestic animals’
2.
Justification of the proposed programme in the light of suggestive training
needs in the discipline (not more than 100 words):
Development and validation of molecular and immunodiagnostic tests for
infectious diseases of domestic animals has shown rapid strides during the past
decade. As a consequence, several validated diagnostic tests having high
predictive values have been made available for applications in management of
clinical cases, epidemiology and control of important infectious diseases,
including transboundry and emerging diseases of animals. The department has
made and is continuing to make significant contributions in development and
application of molecular and immunodiagnostic tests for infectious diseases such as
FMD, PPR, sheep pox, hemorrhagic septicaemia, bluetongue, viral & bacterial diarrhoeas,
mastitis, surra, etc. The proposed topic is covered under the ICAR suggestive list of
topics (Sr. No. 74. ‘Molecular approaches in disease diagnostics and vaccines’).
3.
Venue with full postal/ e-mail address and office phone/fax numbers:
Centre of Advance Faculty Training
Department of Veterinary Microbiology
Lala Lajpat Rai University of Veterinary and Animal Sciences
Hisar- 125 004, Haryana
E-mail: adjitprinik@msn.com, skbatra54@gmail.com
Office Tel: 01662 289319
Telefax: 01662 284312
4.
Proposed dates:
September 17- October 7, 2013
5.
Eligibility qualification for the participants of the Training Programme
i) Master's Degree,
ii) Working not below the rank of Assistant Professor and equivalent in the
concerned subject under Agricultural/ Veterinary Universities /I.C.A.R. Institute
6.
A.
Information regarding proposed Course Coordinator, other than the
Director, CAFT (enclose bio-data clearly bringing out the specific qualification,
experience and scientific contribution of the Course Coordinator in the proposed
topic):
1.
Dr S. K.Batra, Professor
2.
Dr Shiv Charan Gupta, Senior Scientist
Information about Course Coordinator- 1. Dr S.K. Batra
Designation:
Professor (Ex- Head of Department)
Academic Qualifications :
Bachelor of Vet. Sc. & Animal Husbandry (Hons.)
Master of Veterinary Sciences (Veterinary Microbiology)
Ph D (Virology) Univ. of Glasgow (U.K.)
Professional Experience:
Teaching and Research - 32 years
Page 1 of 9
CAFT training in Veterinary Microbiology: Proposal for the year 2013-13. Department of Veterinary Microbiology,
LLRUVAS, Hisar-125004 (Haryana)
Research contribution to the proposed area of proposed topic:
The course coordinator has a vast experience of working with pox viruses, parapox
viruses, enteroviruses and PPR virus of sheep and goats. He has been working with pox
viruses of sheep and goats for a decade. A number of outbreaks of sheep pox and goat
pox infections were investigated for their viral aetiology. Out breaks of parapox
infections in sheep were also investigated. The poly peptides made by the parapox
isolates in cell culture were studied in the lab by employing SDS PAGE analysis.
Immunogenic poly peptides were then identified using Western blot technique. Fusion
protein gene of the sheep pox virus was cloned in PEX vector and expressed in E. coli.
The expressed protein was then used as antigen for detection of anti sheep pox virus
antibodies in the sheep flocks. Lymphocyte profiles in sheep pox virus infected animals
were also studied. Currently investigation on standardization of a quick and cost
effective test for the detection of anti-PPR virus antibodies in the sera of small ruminants
is being undertaken.
Research publications related to the proposed topic:
Kumar, P; Batra, S.K. 2004. Upregulation of T lymphocyte responses and nitric oxide
production in lambs upon infection with CPD virus. J. Immunol. Immunopathol. 6:
551.
Kumar, P; Batra, S.K. and Sharma, R. 2002. Cellular immune response to contagious
pustular dermatitis virus. J. Immunol. Immunopathol. 4(1&2): 43-46.
Kumar, P; Batra, S.K. and Sharma, R. 2001. Alterations in T lymphocyte sub-population in
lambs experimental infected contagious pustular dermatitis virus. I. Immunol.
Immunopathol. 3: 22-27.
Khatri, M; Chand, P. and Batra; S.K. 2001. Western blot analysis of soluble and structural
proteins of contagious pustular dermatitis virus. Israel J. Vet. Med. 56: 90-94.
Khatri, M; Sharma, R; Batra, S. K. and Chand, P. 2000. Cytokines restore infectious bursal
disease virus induced immunosuppression in in-vitro assays J. Immunol.
Immunopathol. 2: 78 – 83.
Khatri, M; Jain, V.K; Batra, S.K; Chand, P. and Khatri, N. 2000. A severe outbreak of
contagious pustular dermatitis in a goat flock. Inter. J. Anim. Sc. 15 : 273 – 274.
Sachan, A.; Khatri, M; Sharma, R. and Batra, S. K. 2000. Sheep pox virus infection alters T
lymphocyte sub population in sheep. Inter. J. Anim. Sc. 15: 105 – 108.
Khatri, M; Chand, P. and Batra, S. K. 1999. Confirmatory diagnosis of contagious pustular
dermatitis virus by electron microscopy. Ind. J. Anim. Sc. 69: 498 – 500.
Batra, S.K; Chand, P. and Rajpurohit, B. S. 1999. A severe outbreak of contagious pustular
dermatitis in a sheep flock. Ind. J. Anim. Sc. 69: 34- 35
Malik, Y; Chand, P. and Batra, S. K. 1998. Isolation and identification of a virus from
severe outbreak of pox in sheep. Ind. J. Anim. Sc. 68:1207 – 1209.
Chauhan, R, S; Batra, S.K; Sharma, R. and Chand, P. 1998. Investigation of an outbreak of
pox in racing pigeons. Ind. J. Anim. Sc. 68: 539 – 541.
Malik,Y; Khatri, M; Batra, S.K. and Chand, P. 1997. Occurrence of sheep pox in
unorganized flocks in Haryana State. Ind. J. Anim. Sc. 68: 962 – 963.
Kaul, A. and Batra, S. K. 1997. Expression of capripox fusion protein and its immune
response in lambs. Int. J. Anim. Sc. 12: 103 – 106.
Batra, S.K; Chand, P. and Rajpurohit, B.S. 1996. Outbreak of capripox in a vaccinated
sheep flock. Ind. J. Vet. Res. 5: 39 – 44.
Batra, S. K. 1994. Sheeppox a dreaded viral disease of sheep. Haryana Farming. 23: 20.
Batra, S. K. and Kalra, S.K. 1990. Adaptability of capripox virus in continuous cell line of
ovine origin. Ind. J. Microbiol. 30: 86 – 88.
Jain,N.C. and Batra, S. K. 1985. Characterization of ovine enteroviruses. Ind. J. Virol. 1:
17 – 25.
Page 2 of 9
CAFT training in Veterinary Microbiology: Proposal for the year 2013-13. Department of Veterinary Microbiology,
LLRUVAS, Hisar-125004 (Haryana)
Jain, N.C; Batra, S. K. and Ram, G.C. 1984. Isolation and characterization of enteroviruses
from neonatal kid diarrhoea. Int J. Diarrh. Dis. Res. 2: 43 – 46.
Papers presented at the national and international conferences:
Karale, P.V. and Batra, S.K. 2011. Comparative evaluation of HI, c-ELISA and SNT for
detection of anti peste des petits virus antibodies in goats. Paper presented at XX
National conference of Indian Virological Sciety (Virocon-2011) held at NRCE, Hisar.
Abs pp 58-59
Kumar, P. and Batra, S. K. 2001. Contagious pustular dermatitis virus increases
production of nitric oxide by the epithelial blood monolayer cells. Second Annual
Conference of SIIP.
Batra, S. K. and Chand, P. 2000. Virological investigation of an outbreak of scabby mouth
in a goat flock. XX Annual Con. of IAVMI and National Symposium on Trends in
Vaccinology in Animal Diseases. Oct. 14-16, pp 79.
Khatri, M; Chand, P. and Batra, S. K. 1998. Analysis of antigenic nature of contagious
dermatitis virus proteins. Eighth International Congress on Infectious Diseases.
Boston, USA 1998. Abs.no. 54045.
Malik,Y; Khatri, M; Batra, S. K. and Chand, P. 1997.Prevalence of sheep pox in Haryana
State. XII Annual Convention of IVS, Gujrat. 1997.
Malik,Y;. Chand, P and Batra, S. K..1997. Detection of A type inclusions in LT cell
monolayers infected with a local isolate of sheep pox virus. IAVMI Annual
Conference, Nov.97.
Batra, S.K; Chand, P. and Rajpurohit, B.S. 1995. Capripox virus outbreak in a vaccinated
sheep flock. 36th Ann. Conf. of AMI pp 282.
Batra,S.K. and Kalra, S.K. 1989. Susceptibility of ovine kidney cells to capripox infections.
IX Annual Conf. and National Symp. of IAVMI, pp21
Jain, N.C; Batra, S. K. 1983. Sheep enterovirus isolation and characterization from
different geographical regions. Ann. Virol. Conf. of IVS.
Jain, N.C. and Batra. S.K. 1982.Studies on neonatal kid diarrhoea virus 4th Ann conf. and
National Symposium of IAVMI. pp19.
Batra,S.K. and Jain,N.C. 1981. Suitability of lamb tissues for goat picorna viruses. 3rd Ann.
Conf. of IAVMI. Pp 67.
B.
Information about Course Co-Coordinator- 2. Dr Shiv Charan
Designation: Senior scientist [Professor]
Academic Qualifications:
Degree
Year
Institute (Area of research)
B.V.Sc.&A.H.
1976
Haryana Agril. University, Hisar
M.V.Sc.(Vet Microbiology)
1978
Haryana Agril. University, Hisar
Ph.D. (Microbiology)
1983
All India Inst. of Medical Sciences, New Delhi
Post-doc Research Fellow
July,1983-Aug.,
Univ. Zurich, Switzerland
85
(Research work on anti-viral Immunity)
Post-doc Research Fellow
Aug., 1992-Sept., Royal Vet College, Univ. London, U.K.
95
(Research on equine herpes virus)
Post-doc Research Fellow
Oct., 1995Univ. Berne, Switzerland
March, 97
(Research on Caprin arthritis-encephalitis)
Professional Experience:
Teaching and Research > 30 years
Research contribution to the proposed areas of topic:
This course coordinator has extensive exposure on various aspects of the mechanisms of
host-defence against microbial infections including T- and B-cell memory, T-B cell
cooperation, hapten-carrier effect and immuno-suppression employing the vesicular
Page 3 of 9
CAFT training in Veterinary Microbiology: Proposal for the year 2013-13. Department of Veterinary Microbiology,
LLRUVAS, Hisar-125004 (Haryana)
stomatitis virus model ( J. Virol 57:1139; 61:2509; PNAS (USA) 83:2604; J. Immunol
136:3057; Eur J Immunol 18:511; 20:2547). In horses, during the equine herpesvirus-1
infection, the precise role of immuno-suppressive cytokines viz. transforming growth
factor-β, that may be induced due to vaccine or with the challenge virus itself, has been
identified as to why in spite of protective T-cell response the animal fails to protect.
(Immunology 90:586). In goats investigations using caprine arthritis encephalitis virus
induced arthritis as a model for rheumatoid arthritis showed an interesting correlation
between T-cell reactivity against CAEV and the severity of arthritis (Vaccine 24:597606).
The current research interests include the development of strategies for the improved
immunogenicity of the currently available vaccine against Pasteurella multocida
involving development a laboratory model, identification of the protective epitope
/peptide and a suitable adjuvant (Ind J. Expt Biology 48:1181; Vet Res Comm 35:457 &
ibid 36:in press). In overall the course coordinator has vast experience in the
development of diagnostic tools for a number of infectious diseases as evidenced by the
publication in the international journals ( ref 8,16, 20-22).
Research publications related to the proposed topic:
FULL LENGTH PAPERS:
Kharb S & S Charan. (2011). Mucosal immunization provides better protection than
subcutaneous immunization against Pasteurella multocida (B:2) in mice preimmunized with the outer membrane proteins. Veterinary Research
Communication.35:457-461. 2010.
Kharb S & S Charan. 2010. Immunogenicity of iron-regulated outer membrane ptoreins
of Pasteurella multocida B:2 im mice model. Indian J Experimental Biology, 48:11811187.
Fluri A, C Nanci, M-L Zahno, H-R Vogt, S Charan, A Bursato, G Pancino, E Peterhans, G
Obexer-Ruff & G Bertoni, 2006. The MHC-haplotype influences primary, but not
secondary memory, immune responses to an immunodominant peptide containing
T- and B- cell epitopes of caprine arthritis encephalitis virus gag protein. Vaccine,
24:597-606.
Poonia B & S Charan. 2005. Early and transient induction of nitic oxide ( NO ) in
infectious bursal disease virus infection is T-cell dependent: a study in cyclosporinA treated chicken model. Indian J Experimental Biology, 43:192-196.
Poonia B & S Charan. 2004. Infiltration by CD4+and CD8+ lymphocytes in bursa of
chickens infected with infectious bursal disease virus ( IBDV ) – strain-specific
differences. Indian J Experimental Biology, 42:823-829.
Poonia B & S Charan. 2001. T- cell suppression by cyclosporin-A enhances infectious
bursal disease virus infection in experimentally infected chickens. Avian pathology,
30:311-319.
Charan S, K Palmer, P Chester, AA Mire-sluis, A Meager & N Edington. 1997.
Transforming growth factor-β1 induced by live or ultraviolet-inactivated equid
herpesvirus type-1 mediates immunosuppression in the horse. Immunology,
90:586-591.
Richa, YP Grover & S Charan. 1993. A dot immunobinding assay in comparison with agar
gel immunodiffusion test for the detection of equine herpesvirus type-1 from field
cases. Scientific and Technical Reviews of Office of International Epizootics (Paris),
12:923-930.
Singh M & S Charan.1992. Immunokinetics of equine herpesvirus type-1 in donkey
mares: suppression of secondary cellular responses. Scientific and Technical
Reviews of Office of International Epizootics (Paris), 11:901-908.
Page 4 of 9
CAFT training in Veterinary Microbiology: Proposal for the year 2013-13. Department of Veterinary Microbiology,
LLRUVAS, Hisar-125004 (Haryana)
Roost HP, Charan S & RM Zinkernagel. 1990. Analysis of the kinetics of antiviral memory
T-cell help in vivo: characterization of short lived cross-reactive T help. European J
Immunology, 20:2547-2554.
Roost HP, S Charan, R Gobet, R Reuedi, H Hengartner, A Althage & RM Zinkernagel. 1988.
An acquired immune suppression in mice caused by infection with lymphocytic
choriomeningitis virus. European J Immunology, 18:511-518.
Charan S, H Hengartner & RM Zinkernagel. 1987. Antibodies against the two serotypes of
vesicular stomatitis virus measured by enzyme-linked immunosorbent assay:
Immuno-dominance of serotype-specific determinants and induction of
asymmetrically cross-reactive antibodies. J Virology, 61:2509-2514.
Charan S & RM Zinkernagel. 1986. Antibody mediated suppression of secondary IgM
response in nude mice against vesicular stomatitis virus. J Immunology, 136:30573061.
Charan S, H Hengartner & RM Zinkernagel. 1986. Primary antibody responses to a welldefined and unique hapten are not enhanced by pre-immunization with carrier:
Analysis in a viral model. Proceedings of National Academy of Sciences (USA),
83:2604-2608.
Charan S, A Huegin, A Cerny, H Hengartner & RM Zinkernagel. 1986. Effect of
cyclosporin-A on humoral immune response and resistance against vesicular
stomatitis virus in mice. J Virology, 57:1139-1144.
Charan S & OP Gautam. 1984. Applications of enzyme-linked immunosorbent assays in
veterinary medicine: a bibliography. Veterinary Research Communication, 8:255267.
Charan S, VM Mahajan, A Rai & S Balaya. 1984. Ocular pathogenesis of Newcastle disease
virus in rabbits and monkeys. J Comparative Pathology, 94:159-163.
Charan S & VM Mahajan. 1983. Pathogenesis of Newcastle disease virus in cell mediated
immune response deprived mice. J Veterinary Medicine (West Germany), 30B: 462465.
Charan S, YP Grover & S Prasad. 1983. Efficacy of double F (Lentogenic) vaccination in
chickens against Newcastle disease. Indian J Poultry Sciences, 18:111-113.
Charan S, A Rai & VM Mahajan. 1983. Comparison of seroreactivity of Lentogenic,
Mesogenic and Velogenic strains of Newcastle disease virus by enzyme linked
immunosorbent and haemagglutination inhibition assays on human sera. J
Veterinary Medicine (West Germany), 30B: 55-60.
Rai A, S Charan & VM Mahajan. 1982. Enzyme-linked immunosorbent assay for the
detection of chlamydial antibodies in poultry associated individuals. Indian J
Medical Research, 76:393-397.
Charan S & VM Mahajan. 1981. Comparison of enzyme-linked immunosorbent assay and
haemagglutination inhibition test for the detection of Newcastle disease virus
antibodies in human sera. J Clinical Pathology, 34:90-92.
Charan S, A Rai, LP Aggarwal & VM Mahajan. 1981. Complement in aqueous humor and
serum of rabbits and monkeys. Indian J Ophthalmology, 28:207-209.
Charan S, VM Mahajan & LP Agarwal. 1981. Newcastle disease virus antibodies in human
sera. Indian J Medical Research, 73:303-307.
Charan S & S Prasad. 1981. Studies on vaccination of pigs with attenuated foot and
mouth disease virus passaged in mice brain. Indian J Animal Sciences, 51:10701074.
SHORT COMMUNICATIONS:
Kumar R & S Charan. 2001. Virus enhancement following infection with antibody-coated
infectious bursal disease virus in chickens. Indian J Experimental Biology, 39:13141317.
Page 5 of 9
CAFT training in Veterinary Microbiology: Proposal for the year 2013-13. Department of Veterinary Microbiology,
LLRUVAS, Hisar-125004 (Haryana)
Charan S, A Rai, VM Mahajan & LP Agarwal. 1981. Modified single radial haemolysis
technique: measurement of antibodies to Newcastle disease virus. J Immunological
Methods, 40:239-242.
Charan S & S Prasad. 1981. Applicability of conglutinating complement adsorption test to
pig serum in foot and mouth disease. Indian J Experimental Biology, 19:181-182.
Charan S & S Prasad. 1981. A note on assessment of post-challenge viraemia as an index
of protective immunity in pigs against foot and mouth disease. J Veterinary Medicine
(West Germany), 28B: 260-262.
Charan S, S Prasad, SC Tewari & Rama kant. 1980. A note on adaptation of a pig strain of
foot and mouth disease virus in baby mice brain. Indian J Animal Sciences, 50:569570.
7.
Sr.
No.
1
Faculty Staff strength in CAFT: 14 (Fourteen)
Assistant Professor
: 1
Associate Professor
: 2
Professor and equivalent : 11
Name and Qualification
Designation
Dr. Ajit Singh, B.Sc., B.V.Sc. & A.H.,
M.V.Sc., Ph.D. (India/UK), PDF (UK)
Professor
Head
2
Dr. Ravindra Sharma, B.V.Sc. & A.H.,
M.V.Sc., Ph.D. (UK)
Sr. Scientist
3
Dr. Satish K. Batra, B.V.Sc. & A.H., M.V.Sc.,
Ph.D. (UK)
Dr. Yash Pal Grover, B.V.Sc. & A.H.,
M.V.Sc., Ph.D. (France)
Dr. Arvind Kumar, B.V.Sc. & A.H., M.V.Sc.,
Ph.D. (UK)
Professor
6
Dr. Sanjay Kapoor, B.V.Sc. & A.H., M.V.Sc.,
Ph.D. (UK)
Professor
7
Dr. Surinder K.Kadian, B.Sc., B.V.Sc. &
A.H., M.V.Sc., Ph.D. (UK)
Dr. Shiv Charan Gupta, B.V.Sc. & A.H.,
M.V.Sc., Ph.D. (India), PDF (Switzerland,
UK)
Dr. Naresh K. Kakker, B.V.Sc. & A.H.,
M.V.Sc., Ph.D. (UK), PDF (UK)
Professor
Sr. Scientist
01662-284333; 9466265761
nkmksk@rediffmail.com
10
Dr. Jagveer Rawat, B.V.Sc. & A.H., M.V.Sc.,
Ph.D. (UK)
Associate
Professor
09468044373
ficats@rediffmail.com/
11
Dr. (Mrs) Archana Sharma, B.V.Sc. & A.H.,
M.V.Sc., Ph.D. (India)
Dr (Mrs) Anshu Sharma, B.V.Sc. & A.H.,
M.V.Sc., Ph.D. (UK)
Associate
Professor
Sr. Scientist
01662-276076; 9416867562
abharadwaj@scientist.com
01662-243786; 09812066778
anshusharma_dr@yahoo.com
13
Dr. Puran Chand, B.V.Sc. & A.H., M.V.Sc.,
Ph.D. (UK)
Sr. Scientist
01662-289320
cpuran@gmail.com/
14
Dr Rajesh Chhabra, B.V.Sc. & A.H.,
M.V.Sc., Ph.D. (India)
Asstt. Scientist
01662-289320
rchhabra59@rediffmail.com/
4
5
8
9
12
&
Contact details
(Phone/Mob/E-mail)
01662-289319;01662288266;
+919896176392;
adjitprinik@msn.com/
01662-284333;01662243786
09896823198
rsharma698@gmail.com/
01662-239244
skbatra54@gmail.com
01662-244496; 9813481628
drypgrover@gmail.com
9896030778
arvind.kumar1956@gmail.co
m/
9354325830
sanjaykapoor59@rediffmail.c
om/
9896689999
skkadian@hau.ernet.in
01662-270088; 9996630017
shivcharan55@yahoo.com
Professor
Sr. Scientist
Sr. Scientist
Page 6 of 9
CAFT training in Veterinary Microbiology: Proposal for the year 2013-13. Department of Veterinary Microbiology,
LLRUVAS, Hisar-125004 (Haryana)
8.
Information regarding other academic staff of the host Institute, who are
likely to be used as resource persons:
Academic staff from Departments of Animal Biotechnology, Veterinary Public
Health & Epidemiology, LLRUVAS, Hisar, Veterinary Parasitology and other
Scientists from local ICAR institute shall be invited during the training.
9.
Specific facilities available for conducting the Programme such as
laboratory equipments/instruments, research farm, library, classroom,
guest-house etc.:
Department of Veterinary Microbiology is CAS/CAFT since 1995 and has so far
successfully conducted 25 trainings for mid-carrier Scientists from SAUs and
ICAR institutes. The Department is well-equipped for conducting the proposed
CAFT training with the laboratory equipments/instruments, class/lecture rooms
with multimedia and other related facilities. Further, the Animal Science section
of COVS has well maintained Animal Farm. The LLRUVAS is located within the
campus of CCS HAU, Hisar and all the common facilities viz. Nehru Library,
Faculty House, etc. are available for use for the participants of CAFT. The
department has recently gone for purchasing ‘smart class room’ facilities.
10.
Programmes/Projects and achievements in the area of special topic
proposed for the training programme:
The department has made significant contributions to the field of modern
molecular and immunological tests for infectious disease diagnosis of important
animal diseases. Notable contributions are in the area of rotavirus, coronavirus,
blue-tongue, pox viruses of animals particularly buffalopox and sheep & goat pox
viruses, FMD, PPR, mastitis-causing agents, equine herpes virus, colibacillosis,
salmonella infections, hemorrhagic septicaemia, etc. Hybridoma technology for
monoclonal antibody production and phage display technology for single-domain
antibody production have been with funding from DBT, Government of India and
are being used in the department since then. Diagnostic tests based on these
reagent antibodies have been developed. RNA electropherotyping and PCR based
molecular tests have been introduced for infectious disease diagnosis. Real-time
PCR and LAMP are currently being undertaken for development of diagnostic
tests for some important diseases in a collaborative research project with Deptt.
of Animal Biotechnology in this university. Regional Research Centre on FMD
through its epidemiological and diagnostic services has made outstanding
contributions in on-going FMD control programme in the state.
11.
Sr
No.
1
2
3
4
5
Tentative Schedule of daily lectures/ practical topics to be covered and name
of the faculty proposed to be engaged during the CAFT Training Programme:
Day/Date Topic
Speaker
&
Designation
17.9.13
Inauguration of the course and lab orientation
Faculty
(Tuesday)
18.9.13
Current trends in diagnostics of infectious diseases of Dr. Ajit Singh*
domestic animals
19.9.13
Recent developments in diagnosis of colibacillosis in Dr Y P
domestic animals
Grover*
20.9.13
Advances in diagnosis of brucellosis in animals
Dr P Chand*
21.9.13
PPR virus infection in small ruminants: diagnosis and Dr S K Batra*
Page 7 of 9
CAFT training in Veterinary Microbiology: Proposal for the year 2013-13. Department of Veterinary Microbiology,
LLRUVAS, Hisar-125004 (Haryana)
6
22.9.13
7
23.9.13
8
24.9.13
9
25.9.13
10
26.9.13
11
12
27.9.13
28.9.13
13
29.9.13
14
30.9.13
15
16
01.10.13
02.10.13
17
18
03.10.13
04.10.13
19
20
05.10.13
06.10.13
21
06.10.13
22
07.10.13
(Monday)
Practicals
1
17.9.13
2
18.9.13
3
19.9.13 &
20.9.13
4
21.9.13
5
22.9.13
6
23.9.13
7
8
9
24.9.13 &
25.9.13
26.9.13
27.9.13
10
28.9.13
11
12
29.9.13
30.9.13
control
Recent developments in diagnosis and control of pox
virus infections in domestic animals
Recent developments in diagnosis and control of
mastitis in farm animals
Current strategies for diagnosis and control of blue
tongue
An update on serodiagnosis of bacterial and viral
diseases of sheep and goat
Recent approaches in diagnosis and control of
mycobacterial infections in animals
Diagnosis and epidemiology of FMD in animals
Caprine arthritis encephalitis virus infection and its
diagnosis
Diagnosis of infectious diseases transmitted through
embryo transfer
Diagnosis of common fungal infections in animals
Dr Naveen
Kumar**
Dr Anshu
Sharma*
Dr Sushila
Maan*
Dr P
Dwiwedi**
Dr S V Singh**
Dr R Sharma*
Dr S Charan*
Invited
speaker**
Dr Archana
Sharma*
Pathobiology of slow virus infections in farm animals
Dr N Kakkar*
Advances in diagnosis of parasitic infections of farm Dr R Garg1
animals
Rabies in farm animals: diagnosis and control
Dr S R Garg2
Trends in diagnosis and control of Japanese encephalitis Dr B R Gulati3
in animal
Advances in diagnosis of rotavirus infections in bovines Dr S Kapoor*
Conventional and molecular methods for diagnosis of Dr Arvind
haemorrhagic septicaemia of cattle and buffaloes
Kumar*
Cytokine – based study of cellular immunity of Dr J Rawat*
infectious diseases of domestic animals: role in
diagnosis
Conclusion
PCR for detection of Pasteurella multocida B:2 in blood
LAMP assay for diagnosis of PPR
Hands on training
Application of fluorescent polarization
diagnosis of brucellosis in animals
Different methods of diagnosis of mastitis
Dr Ajit Singh
Dr Ajit Singh
Dr Ajit Singh
assay
in Dr Puran
Chand
Dr Anshu
Sharma
Use of flow cytometry in diagnosis of mastitis
Dr Anshu
Sharma
Hands on training
Dr Anshu
Sharma
Competitive ELISA for detection of PPR infection
Dr S K Batra
Detection of antibodies against structural proteins of Dr R Sharma
FMD in sheep by liquid phase blocking ELISA
Demonstration of carrier status of sheep for FMD by Dr R Sharma
NSP- ELISA
Hands on training
Dr R Sharma
RT-PCR based diagnosis of blue tongue virus infection
Dr Sushila
Page 8 of 9
CAFT training in Veterinary Microbiology: Proposal for the year 2013-13. Department of Veterinary Microbiology,
LLRUVAS, Hisar-125004 (Haryana)
Maan
13
01.10.13
Lympho-proliferative assay for detection of FMD virus Dr S K Kadian
infection
14
02.10.13
Isolation and identification of dermatophytes of small Dr Archana
ruminants
Sharma
15
03.10.13
Serodiagnosis of Japanese encephalitis in animals
Dr B R Gulati
16
04.10.13
Detection of mycobacterial infection by DTH reaction
Dr J Rawat
17
05.10.13
Monoclonal antibody-based ELISA in detection of Dr Arvind
Pasteurella multocida B:2 antibodies in buffalo sera
Kumar
18
06.10.13
Hands on training & Presentation by the participants
Participants
19
07.10.13
Conclusion
*Designations in the list above; **Invited speakers from outside; 1: Professor, Veterinary
Parasitology; 2: Professor, Veterinary Public Health; 3: Principal scientist, NRCE, Hisar
12.
1
2
3
Name of the Trainings organized during the last three years:
‘Modern
1
trends in vaccines and
diagnostics for the control of
infectious diseases in animals’
‘Molecular
2
and cellular immunology
techniques in animal health’
‘Control
2
of infectious diseases of
animals, with particular emphasis
on FMD Control Programme’
Dr. Shiv Charan & Dr. Ajit March 5-25, 2010
Dr. S.K. Batra
Singh
Dr. S. K. Kadian &
Dr. Jagveer Rawat
Dr. R. Sharma and
Dr. N. K. Kakker
Dr. Ajit Nov. 3-23, 2011
Singh
Dr. Ajit Sept. 18- Oct. 8,
Singh
2012
13. Signature of the Director of the CAFTs (With official Seal):
-signed(Dr Ajit Singh)
Prof. & Head-cumDirector, CAFT
Deptt. of Vet.
Microbiology
LLRUVAS, Hisar
Dated: 11.2.1013
Page 9 of 9