Table S1_Laviale et al.
In situ light and temperature parameters during a summer diurnal emersion in the
studied Portuguese (PT-Ria de Aveiro, Vista Allegre) and French (FR-Baie de
l’Aiguillon, Esnandes) intertidal flats. In comparison, the experimental light dose was 12.96
mol photons m-2 (i.e. 3 h of 1200 µmol photons m-2 s-1).
PT
FR
Light dose over emersion (mol photons m-2)
19.47
22.96
Time for experimental light dose (hh : mm : ss)
02 : 53 : 26
03 : 34 : 35
Average (min:max) surface temperature over emersion (°C)
37.5
28.8
(27.8 : 42.2)
(20.7 : 34.8)
30.2
22.7
(25.7 : 31.5)
(20.0 : 25.0)
Average (min:max) temperature at -0.5 cm over emersion
(°C)
Table S2_Laviale et al.
Relative abundances of benthic diatoms species sampled in the first cm of the sediment
in the studied Portuguese (PT-Ria de Aveiro, Vista Allegre) and French (FR-Baie de
l’Aiguillon, Esnandes) intertidal flats. Identified species were epipelic (i.e. motile), unless
stated as phytoplanktonic (*) or thychoplanktonic species (**); s.l.: sensu lato. ‘Other’ brings
together diatoms species with a relative abundance < 1%.
Species name
PT site
Navicula phyllepta s.l.
Melosira sp.*
Staurophora amphioxys
Diploneis didyma
Amphora spp.
Nitzschia acuminata
Nitzschia sp. 1
Psammodictyon
panduriforme
Paralia sulcata*
Surrirella sp.
Other
FR site
Navicula spartinetensis
Navicula phyllepta s.l.
Plagiotropis seriata
Cymatosira belgica**
Nitzchia sp. 2
Psammodictyon
panduriforme
Thalassiosira sp.*
Other
Relative abundance
(%)
44.2
9.4
8.4
7.3
6.5
5.8
4.5
4.2
3.1
1.0
5.5
33
28
8
5
4
4
3
15
Table S3_Laviale et al.
Outputs of the statistical analysis performed on FR data set (a: PSII; b: NPQ; c: Chl a
allomerization ratio (AR); d: DD+DT pool; e: DD de-epoxidation state (DES); f: TBARS
content (MDA). Time (T0, after 3 h of HL, after 15 min recovery under LL), temperature
(27°C and 37°C) and chemical (Ctrl or +Lat A) were considered as fixed factors. The fact that
PSII and NPQ measurements were made on the same samples over the experiment was also
taken into account (i.e. related samples). Level of significance indicated as: ‘***’ (P < 0.001),
‘**’ (P < 0.01), ‘*’ (P < 0.05).
a
PSII
time
temperature
chemical
time x temperature
time x chemical
temperature x chemical
time x temperature x chemical
b
Pr (> F)
< 2e-16
6.94E-11
0.0014
2.30E-10
0.0217
0.0937
0.3754
significance
***
***
**
***
*
df
2
1
1
2
2
1
2
F value
905.5
77.3
7.6
28.0
2.4
0.9
0.4
Pr (> F)
< 2e-16
5.72E-09
0.0110
5.35E-07
0.1120
0.35200
0.700
significance
***
***
*
***
df
2
1
1
2
2
1
2
F value
19.0
0.5
10.5
0.7
2.9
0.8
0.2
Pr (> F)
9.50E-06
0.4762
0.0033
5.28E-01
0.0727
0.39342
0.800
significance
***
df
2
F value
109.9
Pr (> F)
2.90E-14
significance
***
AR
time
temperature
chemical
time x temperature
time x chemical
temperature x chemical
time x temperature x chemical
d
F value
703.3
135.8
13.4
71.7
4.6
3.1
1.3
NPQ
time
temperature
chemical
time x temperature
time x chemical
temperature x chemical
time x temperature x chemical
c
df
2
1
1
2
2
1
2
**
DD+DT pool
time
temperature
chemical
time x temperature
time x chemical
temperature x chemical
time x temperature x chemical
e
7.3
0.4
2.9
0.1
0.1
0.4
0.0113
0.5374
0.0695
0.8801
0.7969
0.6709
*
df
2
1
1
2
2
1
2
F value
398.6
197.8
113.2
70.0
43.8
26.2
19.0
Pr (> F)
< 2e-16
2.23E-13
9.02E-11
5.69E-11
6.78E-09
2.72E-05
9.73E-06
significance
***
***
***
***
***
***
***
df
2
1
1
2
2
1
2
F value
0.4
1.7
0.0
0.1
4.3
0.1
1.6
Pr (> F)
0.7055
0.1934
0.9933
0.9092
0.0202
0.792
0.2201
significance
DES
time
temperature
chemical
time x temperature
time x chemical
temperature x chemical
time x temperature x chemical
f
1
1
2
2
1
2
MDA
time
temperature
chemical
time x temperature
time x chemical
temperature x chemical
time x temperature x chemical
*
Table S4_Laviale et al.
Outputs of the statistical analysis performed on data gathered after 15 min recovery
under LL (a: PSII; b: NPQ). Site (PT or FR), chemical (Ctrl or +Lat A) and temperature
(27 or 37°C) were considered as fixed factors. Level of significance indicated as: ‘***’ (P <
0.001), ‘**’ (P < 0.01), ‘*’ (P < 0.05).
a
PSII
site
temperature
chemical
site x temperature
site x chemical
temperature x chemical
site x temperature x chemical
b
df
1
1
1
1
1
1
1
F value
148.9
119.2
38.9
38.2
7.5
0.1
4.2
Pr (> F)
< 2e-16
2.94E-16
3.93E-08
4.90E-08
0.00817
0.73927
0.04334
significance
***
***
***
***
**
df
1
1
1
1
1
1
1
F value
12.5
78.9
15.7
1.1
0.04
1.5
0.04
Pr (> F)
0.00276
1.38E-07
0.00112
0.30759
0.85480
0.23905
0.85480
significance
**
***
**
*
NPQ
site
temperature
chemical
site x temperature
site x chemical
temperature x chemical
site x temperature x chemical
Figure S1_Laviale et al.
A
B
AR (%)
20
27°C
FR Ctrl
FR + Lat A
20
15
15
10
10
5
5
0
T0
HL
Recov
LL
0
37°C
T0
HL
Recov
LL
Pigment content makers of photooxidative stress (Chl a allomerization ratio, AR) of
microphytobenthos harvested at the French site (FR-Baie de l’Aiguillon, Esnandes)
during light stress recovery experiments performed on sediment exposed at two
temperatures (27°C, A- or 37°C, B-) and with an inhibitor of cell motility (+Lat A) or
without (Ctrl). T0, beginning of the experiment under low light (50 µmol photons m-2 s-1);
HL, after 3 h high light (1200 µmol photons m-2 s-1); Recov, after 15 min recovery under low
light (50 µmol photons m-2 s-1) following the 3 h high light; LL, after 3 h of low light (50
µmol photons m-2 s-1). Values are mean ± SD (n=6).
Figure S2_Laviale et al.
B
DD+DT (mol. 100 mol Chl a-1)
A
80
80
37°C
27°C
FR Ctrl
FR + Lat A
60
60
40
40
20
20
0
T
0
HL
Recov
LL
0
T0
HL
Recov
LL
Pigment content markers of photoprotection (DD+DT pool normalized to total Chl a) of
microphytobenthos harvested at the French site (FR-Baie de l’Aiguillon, Esnandes)
during light stress recovery experiments performed on sediment exposed at two
temperatures (27°C, A- or 37°C, B-) and with an inhibitor of cell motility (+ Lat A) or
without (Ctrl). Labels as in Fig. S1. Values are mean ± SD (n=6).
Figure S3_Laviale et al.
A
B
80
27°C
DES (%)
FR Ctrl
FR + Lat A
80
37°C
60
60
40
40
20
20
0
T0
HL
Recov
LL
0
T0
HL
Recov
LL
Pigment content markers of photoprotection (DD de-epoxidation state, DES, in mol 100
mol Chl a-1) of microphytobenthos harvested at the French site (FR-Baie de l’Aiguillon,
Esnandes) during light stress recovery experiments on sediment exposed at two
temperatures (27°C, A- or 37°C, B-) and with an inhibitor of cell motility (+ Lat A) or
without (Ctrl). Labels as in Fig. S1. Values are mean ± SD (n=6).
Figure S4_Laviale et al.
2
- Lat A
NPQ = -3.41 + 0.21 DES R = 0.83
+ Lat A
NPQ = -1.18 + 0.08 DES R = 0.92
2
5
NPQ
4
- Lat A
+ Lat A
3
2
1
0
0
10
20
30
40
50
60
70
80
DES (%)
DES (DD de-epoxidation state, DES, in mol. 100 mol Chl a-1) versus NPQ (nonphotochemical quenching of Chl a fluorescence, an index of photoprotection)
relationship for MPB harvested at the French site (FR-Baie de l’Aiguillon, Esnandes)
during light stress experiments (LSRE) performed on sediment exposed at two
temperatures (27°C or 37°C) and with an inhibitor of cell motility (+ Lat A) or without
(Ctrl). Data are from Fig. S3 and Fig. 3.
Figure S5_Laviale et al.
MDA equivalent / Idiat
5
PT + Lat A, 27°C
PT + Lat A, 37°C
4
3
2
1
0
T0
HL
Recov
LL
Amount of lipid peroxidation marker (TBARS content in 10-6 nmol MDA equivalent
mL-1) in MPB harvested at the Portuguese site (PT-Ria de Aveiro, Vista Allegre) during
light stress experiments (LSRE) performed on sediment exposed at two temperatures
(27°C, A- or 37°C, B-) with an inhibitor of cell motility (+ Lat A). Labels as in Fig. S1.
Values are mean ± SD (n=6).