The identification of Trinema species living in Sphagnum and soils Compiled from the following sources: (Grospietsch 1958; Harnisch 1958; Luftenegger et al. 1988; Ogden & Hedley 1980). Disclaimer. This is a grossly simplified key of some main types. The true diversity of the genus is much higher both considering described taxa, and certainly even more so if pseudo-cryptic and cryptic taxa are considered. Still imperfect as this document is hopefully it will be useful. Any suggestions for improvement are very welcome! Copyright Edward Mitchell 2002-. All material can be used freely for non-profit educational purposes. An acknowledgement for such a use would be appreciated. Key 1. Breadth to length ratio > 0.55 (range 0.57-0.62). Oval aperture, surrounded by a thick border: Ratio between the small diameter of aperture and the distance from the top of aperture to the oral end of the test < 2. 2 Breadth to length ratio < 0.55 (range 0.41-0.51). Round aperture, surrounded by a thin border: Ratio between the small diameter of aperture and the distance from the top of aperture to the oral end of the test > 2. 3 2. Shell composed of two independent parts. Outer component carries the circular shell platelets, inner part forms the oval aperture. In side view the outline has the shape of a smooth pointed egg with the two side converging symmetrically towards the anterior end of the test. Aperture clearly invaginated. (Figure 1) T. penardi In side view the outline is not symmetric. The anterior end of the test is very compressed, and the anterior lip of the aperture that creates a distinct thickening on the outline when viewed in profile. Aperture slightly invaginated. (Figure 2) T. complanatum 3. Smaller species. Mean length 35 µm (main range 30-38 µm; extreme range 1641 µm); mean breadth 15 µm (main range 10-18 µm; extreme range 7-21 µm). Breadth to length ratio 0.42 to 0.51. T. lineare Larger species. Mean length 48 µm (main range 45-52 µm; extreme range 32125 µm); mean breadth 20 µm (main range 16-23 µm; extreme range 1560 µm). Breadth to length ratio 0.41 to 0.43. T. enchelys Note: The available biometric data suggests that there is some overlap between these two species for the size range 32-41µm. For specimens within this size no clear identification criteria seems to be available. Ref. Lengt M inh (L) M ax M T. penardii T. com planat um T. enchel ys 48 40 30 1 50. 2 53 26 - 53. 2 31. 8 - - - 50 - - - 47 - 46. 6 - - 48. 4 - - 16 13 25 23 19 16 15 18 10 14 - - - 7 15 8 - 35 29 45 29 34 23 60 20 21 - - 30 16 30 20 20 15 17 17 19 18 19 M Trinem a. Breadth M in (B) M ax 34. 8 34. 4 Aver. - 49 Ogden and Hedley 1980 Gros piet sch 1958 Harni sh 1958 1 2 3 LufCash tenegger et al . 1998 4 et al. 1915 5 65 75 24 30 100 125 103 78 52 38 45 40 47 41 32 52 45 26 21 16 43 35 25 1 1 5 1 1 2 3 4 5 3 4 5 Bi omet ri cal data com pil ed f or 1 four 30spec ies 40 of 35 1 24 T. l ineare 41 35 2 18 35 Speci es Tabl e 1. - - - - - - - 30. 4 19. 3 29. 9 19. 6 19. 9 16. 9 14. 6 Aver. - - - - - - - 15 10 18 14 18 - - - - - - - 8 13 30 33 30 20 20 18 16 Depth (D) M in M ax - - - - - - - M 25 13 25 17 20 - - - - - - 25. 3 15. 0 23. 7 17. 3 19. 6 - - - - - - 8 - 10 - - 11 - 6 7 7 - 6 - 16 - 17 - - 20 - 11 20 11 - 8. 5 - M in M ax M Aver. Widt h of aperture 8 15 14. 10(w) 3 5 14 13. 7 10 3 6 13 13 - - - - - 9 9 - - - 13. 1 13. 0 8. 8 9. 2 - - - - - - - - 5 - 8 - 11 6 7 7 - 6 3 - - 13 - 16 - - 20 - 11 20 11 8. 5 6 10 - 10 - - - - 9 9 - - - 10. 2 10. 4 8. 8 9. 2 - - - - - - - - 5 - - 5 - - 8 - 10 - - - - 4 3 - - - - - - - - - M M M in M ax Mw. M ax in aperture Dis t. Bet Aver. Aver. and anterior end (a) 9 Height 8 9 8. 8 2 5 10(h) 8. 8 of aperture 8 7. 2 5 8 7. 2 2 4 10 B/ L 8 - 7 - - - - 3 6. 9 - - - - - 0. 51 0. 40 0. 39 0. 47 0. 42 0. 53 0. 54 7 0. 66 3. 3 - - - M in Aver. 4 3. 3 0. 50 3 2. 5 0. 33 0. 39 0. 56 0. 48 - 0. 54 0. 60 0. 60 0. 23 0. 44 0. 44 0. 58 0. 38 0. 47 0. 54 0. 49 0. 48 0. 41 M ax - - - 0. 57 0. 62 0. 60 0. 43 0. 41 0. 51 0. 43 M - - - - - - - 0. 57 0. 61 0. 60 0. 42 0. 41 0. 49 0. 42 Aver. - - - - - - - 0. 50 0. 42 0. 47 0. 34 0. 42 0. 43 0. 33 M in D/L - - - - - - 0. 46 0. 69 0. 40 0. 38 0. 38 0. 40 0. 44 M ax - - - - - - - 0. 47 0. 50 0. 50 0. 36 0. 41 0. 43 0. 40 M - - - - - - - 0. 48 0. 47 0. 47 0. 37 0. 40 0. 41 0. 40 Aver. - - - - - - - 1. 00 1. 60 2. 33 4. 00 2. 50 M in h/ a - - - - - 1. 63 1. 60 - - - - - 2. 75 - - - - - 2. 00 2. 50 M ax 1. 25 1. 43 3. 00 2. 25 2. 67 M - - - - - - - - - 1. 48 1. 49 2. 79 2. 67 2. 88 Aver. - - - - - - - - - Figure 1 (left) and 2 (right). Profile outlines of Trinema penardi (left) and T. complanatum (right) References Grospietsch, T. (1958) Wechseltierchen (Rhizopoden) Kosmos Verlag, Stuttgart. Harnisch, O. (1958). II. Klasse: Wurzelfüssler, Rhizopoda. Die Tierwelt Mitteleuropas, Band 1: Urtiere-Hohltiere-Würmer, Lieferung 1b (eds P. Brohmer, P. Ehrmann & G. Ulmer), pp. 1-75, pls. 1-26. Quelle & Meier, Leipzig. Luftenegger, G., Petz, W., Berger, H., Foissner, W. & Adam, H. (1988) Morphologic and Biometric Characterization of 24 Soil Testate Amebas (Protozoa, Rhizopoda). Archiv Fur Protistenkunde, 136, 153-189. Ogden, C.G. & Hedley, R.H. (1980) An atlas to freshwater testate amoebae Oxford University Press, Oxford.