PROGRAMME SATURDAY June 12 14.45-18.00 Registration Mounting of posters 18.15-18.30 Opening of the Workshop Gian Paolo Accotto INTRODUCTORY LECTURES Chair: Gian Paolo Accotto 18.30-19.10 RNA conformational shifts control translation and replication of Turnip crinkle virus (O1) Anne E. Simon 19.10-19.50 Defense, counter-defense and counter-counter-defense: a never ending tale of RNA silencing (O2) Olivier Voinnet 20.30 Dinner 22.00-23.00 Mounting of posters SUNDAY June 13 SESSION: REPLICATION AND TRANSLATION Chair: K. Andrew White 8.30 Tombusvirus recruitment of the host translational machinery via the 3’UTR (O3) K. Andrew White 8.55 Viroid replication: recent insights on the enzymes and ribozymes involved (O4) Ricardo Flores 9.20 Geminivirus infection and plant cell cycle controls (O5) Linda Hanley-Bowdoin 9.45 Host factors required for plant susceptibility to viruses: targets to improve plant resistance to viruses (O6) Carole Caranta 10.10 Post-translational modifications of TYMV polymerase and their role(s) in viral replication (O7) Isabelle Jupin 10.45-11.15 Coffe break 11.15-12.15 Four short talks (15 min each) Specific Interaction with Viral RNA Reveals a Novel Role of Hsp90 in the Initiation of Plant Virus Replication (P3) Y.W. Huang Quantitation of Potato virus A gene expression under various conditions gives new insights into the regulation of viral translation and replication (P4) K. Mäkinen The Cauliflower mosaic virus reinitiation factor TAV controls the TOR signalling pathway (P8) L. Ryabova Viral-induced systemic necrosis in plants involves both programmed cell death and the inhibition of viral multiplication, which are regulated by independent pathways (P40) M. Hashimoto 12.30-14.00 Lunch (buffet) 14.00-15.30 Poster session (P1-P20) Presenters standing near their poster SESSION: PLANT RESPONSE (part I) Chair: Savithramma P. Dinesh-Kumar 15.30 Emerging perspectives on the antiviral innate immune response (O8) Savithramma P. Dinesh-Kumar 15.55 Massive production of small RNAs from a non-coding region as a decoy strategy of Cauliflower mosaic virus to counteract gene silencing (O9) Mikhail M. Pooggin 16.20 RNA silencing suppressors and potyviral infections: a complex tale (O10) Juan A. García 16.45-17.15 Coffee break 17.15 Multiple effects of silencing the RDR1 gene of tobacco (O11) Peter Palukaitis 17.40 Toward understanding the mechanisms of tobamovirus replication complex formation (O12) Masayuki Ishikawa 18.15 Bus leaving for the Visit to the Fortress of Fenestrelle 20.30 Dinner in the Fortress, with music entertainment MONDAY June 14 SESSION: PLANT RESPONSE (part II) Chair: Jari P.T. Valkonen 8.45 Viral Class 1 RNase III enzymes as suppressors of RNA silencing (O13) Jari P.T. Valkonen 9.10 The role of TYLCV V2 in suppression of RNA silencing (O14) Yedidya Gafni 9.35 Mechanisms of argonaute targeting viral suppressors of RNA silencing (O15) Jozsef Burgyan 10.00 Viral secondary siRNAs in antiviral silencing (O16) Shou-Wei Ding 10.25 Organ-specific transcriptional response in tomato systemically infected by Tomato spotted wilt virus (O17) Gian Paolo Accotto 11.00-11-30 Coffee break 11.30-13.00 Six short talks (15 min each) A new potyvirus virulence determinant: the CI C-terminus modulates pathogenicity of Lettuce mosaic virus in lettuce (P18) S. German-Retana Virus-derived small RNAs and their potential to control the outcome of plant-virus interactions (P23) C. Llave Plant virus mediated induction of miR168 is associated with repression of ARGONAUTE1 accumulation (P19) Zoltan Havelda Structural and functional analysis of viral siRNAs (P30) G. Szittya Role of C2 during Geminivirus infection: effect on the CSN and the SCF complexes (P36) R. Lozano-Duran RNA silencing in plant-viroid interactions: insights from deep sequencing of the small RNAs from plants infected by nuclear- and chloroplast-replicating viroids (P17) Francesco Di Serio 13.15-14.45 Lunch (buffet) SESSION: VIRUS / VECTOR INTERACTIONS Chair: Juan José López-Moya 15.00 Vector transmission mechanisms in two genera of the family Potyviridae: differences between potyviruses and ipomoviruses (O18) Juan José López-Moya 15.25 How can a plant virus “sense” the feeding of its insect vector on the infected plant and immediately relocate within the cell and increase its chances of acquisition? (O19) Stéphane Blanc 15.50 Microarray-based analysis of interactions between Tomato yellow leaf curl virus and its vector the whitefly Bemisia tabaci (O20) Henryk Czosnek 16.30-17.00 Coffee break 17.00-17.30 Two short talks (15 min each) What’s new in polerovirus transmission ? (P43) V. Brault Facilitation of Bemisia tabaci transmission of Tomato yellow leaf curl virus by a single symbiotic bacterium (P44) M. Ghanim 17.40-18.20 EMBO Science & Society Lecture: Communicating science: people and biology, hopes, expectations and fears Silvia Rosa-Brusin 18.30 Poster session (P21-P42) Presenters standing near their poster 20.30 Dinner 22.00 Presentation of the next EMBO Workshop Tasting of local food and spirits, also for sale TUESDAY June 15 SESSION: MOVEMENT AND TRAFFICKING Chair: Andrew J. Maule 8.45 Plasmodesmata - Gateways to local and systemic virus infection (O21) Andrew J. Maule 9.10 The cell nucleus and plant virus movement (O22) Michael Taliansky 9.35 Structural and functional insights into tubule-guided virus movement (O23) Christophe Ritzenthaler 10.00 Couple-skating Abutilon mosaic virus movement proteins (O24) Holger Jeske 10.25 Phloem tropism of poleroviruses does not hinge only upon Dicerlike activity (O25) Véronique Ziegler-Graff 11.00-11-30 Coffee break 11.30-13.00 Poster session (P43-P66) Presenters standing near their poster 13.00 Lunch (buffet) 14.30 Departure from Pracatinat to Torino: visit to the town centre and dinner 22.00 Bus departure from Torino to Pracatinat WEDNESDAY June 16 SESSION: RECOMBINATION AND EVOLUTION Chair: Fernando García-Arenal 8.30 Mechanisms and genetic regulation of plant tolerance to virus infection (O26) Fernando García-Arenal 8.55 Adaptation process of Rice yellow mottle virus to two alleles of the eIF(iso)4G-mediated rice resistance (O27) Denis Fargette 9.20 Variability and evolution rate of plant viruses with a singlestranded DNA genome (O28) Bruno Gronenborn 9.45 A systems biology approach to dissect the roles of host proteins in tombusvirus RNA replication (O29) Peter D. Nagy 10.30-11.00 Coffee break 11.00-12.30 Six short talks (15 min each) The cytosolic nucleoprotein of the plant-infecting bunyavirus Tomato spotted wilt is able to recruit ER-resident proteins to ER export sites (P50) Richard Kormelink The intra- and intercellular movement of Melon necrotic spot virus (MNSV) depends on an active secretory pathway (P51) Vicente Pallas Genetic and functional characterization of the RTM-mediated resistance (P52) Frédéric Revers Biological and cellular evaluation of the association of the movement protein of Tobacco mosaic virus to biological membranes (P53) Jesús A. Sánchez-Navarro Nuclear and chloroplastic viroids replicate with different mutation rates (P59) Selma Gago VPg and HCpro: central players in potyvirus-host interactions (P29) Minna-Liisa Rajamäki 13.00 Lunch (buffet) 14.00 Departure of shuttle buses for Torino (airport and city centre)