Human Adhesion 6plex
The interaction between cells via intimate cell-cell contact is facilitated by an
array of cell surface molecules, termed adhesion molecules, which act via ligandreceptor binding. This adhesion assay is a multiplex fluorescent bead based
immunoassay for the quantitative analyte detection by flowcytometry of 6 of
these adhesion molecules and selectins in cell culture supernatants, serum,
plasma, or other body fluids.
Microspheres coated with antibodies specific to each of the analytes can be
differentiated by their sizes and by their distinct spectral addresses. A
fluorescently labeled second antibody mixture binds to the analytes captured by
the first antibodies on the beads. A standard curve is included for each analyte in
the multiplex system. The samples and standards are prepared in a multiwell
plate such that 80 samples can be processed all together. This assay is for
research use only and is not for use in diagnostic or therapeutic procedures.
sICAM-1
sICAM-3
sPECAM
sVCAM-1
sE-selectin
sP-selectin
Standard range (µg/ml) Detection Limit (ng/ml)
0-4
5.3
0-8
4.8
0-4
0.8
0-4
0.2
0-4
1.2
0-14
5.7
Soluble intercellular adhesion molecules -1 and -3 (ICAM-1 and ICAM-3), have
an important role in the initiation of immune responses and thus as disease
markers for various pathological situations.
Soluble vascular cell adhesion molecule-1 (VCAM-1) is increased in autoimmune
diseases, infection, inflammation, and cancers.
Soluble platelet endothelial cell adhesion molecule-1 (PECAM-1) has a role in
the inflammatory process and leukocyte-endothelial interaction.
Soluble endothelial-selectin (E-selectin) mediates the initial interactions of
leukocytes and platelets with endothelial cells. The circulating form exerts
chemotactical signals on neutrophils.
soluble platelet-selectin (P-selectin) is implicated in a number of inflammatory
disorders including diseases of the heart. And in tumor formation and metastasis.