Biotechnology Pre/PostTest Key (w/citations)
Name ________________________________________
May 11, 2013
SC.912.L.16.9 Explain how and why the genetic code is universal and is common to almost all organisms.
SC.912.L.16.3 Describe the basic process of DNA replication and how it relates to the transmission and
conservation of the genetic information.
SC.912.L.16.10 Evaluate the impact of biotechnology on the individual, society and the environment,
including medical and ethical issues.
_____1. The job of the lysis buffer is to:
A. Rinse any remaining debris from the spin column.
B. Break open the cell and nuclear membranes to release DNA.
C. Convert DNA back to its original structure.
D. Remove DNA from the spin column
http://missionbiotech.com
_____2) The purpose of DNA extraction is to:
A. Heat the DNA to bind it with RNA.
B. Remove DNA from the patient sample, leaving only proteins.
C. Isolate DNA strands for use with PCR.
D. Cool the DNA, keeping it in a dormant state for later use.
http://missionbiotech.com
_____3) Which of the following statements about DNA and RNA is NOT true?
A. DNA contains the nitrogenous base uracil;
B. They can both be found in the nucleus of a cell;
C. They can both be found in viruses;
D. RNA can be found in ribosomes.
http://missionbiotech.com
_____4) The rate of migration of DNA within an agarose gel during electrophoresis is primarily based on
what factor??
A. The size of the DNA fragments.
B. The size of the wells in the gel.
C. The volume of the DNA sample loaded
D. The number of DNA fragments.
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_____5) Restriction enzymes:
A. Restrict the length of a growing DNA strand
B. Determine which nucleotides will be added to a growing strand
C. Cut DNA at specific nucleotide sequences
D. Bind DNA fragments
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_____6) Electrophoresis can be useful in as a biotechnology tool for:
A. Diagnosis of inherited disorders.
B. Forensic or criminal applications.
C. Developing cures for inherited disorders.
D. All of the above.
2012 FL Holt McDougal Biology
_____7) PCR is a technique used to:
A. Create a master mix for DNA extraction.
B. Replicate DNA strands millions of times (amplify).
C. Convert DNA into RNA.
D. Rinse debris from DNA.
http://missionbiotech.com
_____8) Short strands of DNA that attach to specific regions of single-stranded DNA are called:
A. Fluorescent dye
B. Nucleotides
C. Polymerase
D. Primers
http://missionbiotech.com
_____9) The enzyme that adds nucleotides to a growing strand of DNA in a PCR reaction is called:
A. Nucleotase
B. Polymerase
C. Primerase
D. Reverse transcriptase
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_____10) What piece of laboratory equipment would you use to heat and cool reactants for a PCR reaction?
A. Centrifuge
B. Spin column
C. Thermocycler
D. Water bath
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_____11) Which of these in NOT an advantage of genetically engineered crop plants?
A. They can produce their own pesticides
B. They can grow larger than unmodified crops
C. They cannot cause an allergic reaction
D. They can contain extra nutrients
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_____12) Consider the following statements about genetic engineering:
I.A.
B.
II.
C.
D.
Piecescan
of DNA
from
twoown
different
organisms can be joined
They
produce
their
pesticides
They can grow larger than unmodified crops
Genescannot
from complex
such as animals can be inserted into simpler organisms
They
cause an organisms
allergic reaction
Theysuch
can as
contain
extra nutrients
bacteria
III. Recombinant DNA is not passed down to offspring during reproduction
IV. Genetic engineering can benefit medicine and society by producing large amounts of
proteins that are used to treat certain diseases.
Which of the statements above are true?
A. I, II, and IV
B. I, III, and IV
C. I and IV only
D. I, II, III, and IV
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