BBMRI-RP3 Data Access Application Form for Access to BBMRI-RP3 Data PLEASE SUBMIT COMPLETED, SIGNED AND DATED FORM BY EMAIL TO: Ri.Jansen@ggzingeest.nl 1. Name and position of the Applicant(s), including employment or affiliation with any organization Please ensure that a full postal and email address is included for each applicant. 2. Title of the study In less than 30 words. 3. Research Question Please describe the study in no more than 750 words. Include: a. outline of the study design; b. an indication of the methodologies to be used; c. proposed use of the Project Data; d. preceding peer-reviews of the study (if any present); e. specific details of what you plan to do with the Project Data; f. timeline; g. key references. 4. Consent and Approvals If your proposed use of Project Data involves use of your own data, please confirm that you have obtained all approvals required by the rules and regulations of your jurisdiction, including your Page 1 of 6 BBMRI-RP3 Data Access institution’s institutional rules, and the consent of your data subjects, for your use of your own data in the study, by ticking the following box: Yes, we have □. 5. The nature and amount of Project Data applied for Please specify the RNA sequencing data you request access to. Please specify the methylation data you request access to. Please specify the genotype data you request access to. Please specify the phenotype data you request access to (see appendix A for available phenotypes). Please specify the GONL RNA sequencing data you request access to Please specify the GONL RNA methylation data you request access to Please specify the Complete Genomics RNA sequencing data you request access to Please specify the Complete Genomics methylation data you request access to 6. Resources, Feasibility & Expertise Please confirm that you have secured funding for your proposed use of the Project Data and that you will carry out your research within a reasonable period of time after the granting of this Page 2 of 6 BBMRI-RP3 Data Access application: by ticking the following box: Yes, we will □. Please describe your experience and expertise, and that of your collaborators, and how this will be applied to the proposed study (in 250 words). Please provide a list of recent publications (max. 10). Page 3 of 6 BBMRI-RP3 Data Access Appendix A: Available phenotypes Anthropometry: Anthropometry_Age xx.x (i.e. years + 1 decimal) Height(cm) Weight(kg) Lipid phenotypes: Lipids_BloodSampling_Age Lipids_BloodSampling_Date Lipids_BloodSampling_Time Lipids_BloodSampling_Fasting TotChol(mmol/L) HDLchol(mmol/L) Triglycerides(mmol/L) LipidsMed_Age LipidMed Technical covariates: Subject_id Ascertainment_criterion Inclusion criterion biobank/selected samples GWAS_ChipType(s) GWAS_DataGeneration_Date DNA_BloodSampling_Age DNA_BloodSampling_Date DNA_BloodSampling_Time DNA_Source Whole blood, other DNA_Extraction_Method DNA_Extraction_Date DNA_QuantificationMethod DNA_A260/A280 Page 4 of 6 BBMRI-RP3 Data Access RNA_BloodSampling_Age RNA_Sampling_Date RNA_Sampling_Time RNA_Source Pax gene, other RNA_Extraction_Date RNA_Extraction_Method RNA_RIN RNA_A260/280 BirthYear xxxx (e.g. 1969) Sex 0=male/1=female Smoking Blood parameters: CRP_BloodSampling_Age CRP_BloodSampling_Date CRP_BloodSampling_Time hsCRP(mg/L) CellCount_BloodSampling_Age CellCount_BloodSampling_Date CellCount_BloodSampling_Time WhiteBloodcellCount-WBC(x10^9cells/L) RedBloodcellCount-RBC(x10^12cells/L) Hemoglobulin-HGB(mmol/L) Hematocrit-HCT(L/L) MeanCorpuscularVolume-MCV(fL) MeanCorpuscularHemoglobin-MCH(fmol) MeanCorpuscularHemoglobineConcentrationMCHC(mmol/L) CorpuscularHemoglobinConcentrationMeanCHCM(mmol/L) Page 5 of 6 BBMRI-RP3 Data Access CorpuscularHemoglobinContent-CH(fmol/L) RedCellVolumeDistributionWidth-RDW(%) HemoglobinConcentrationDistributionWidthHDW(mmol/L) PlateletCount-PLT(x10^9cells/L) MeanPlateletVolume-MPV(fL) NeutrophilCount(10^9/L) LymphocyteCount(10^9/ MonocyteCount(10^9/L EosinophilCount(10^9/L) BasophilCount(10^9/L) LargeUnstainedCells-LUC(10^9/L) Neut(%) Lymph(%) Mono(%) Eos(%) Baso(%) LargeUnstainedCells-LUC(%) Page 6 of 6