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Supplementary materials
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Mangrove Trees Affect the Community Structure and Distribution of Anammox
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Bacteria at an Anthropogenic-Polluted Mangrove in the Pearl River Delta
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Reflected by 16S rRNA and Hydrazine Oxidoreductase (HZO) Encoding Gene
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Analyses
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Meng Li1, Yi-Guo Hong1, 2, Hui-Luo Cao1 and Ji-Dong Gu1, 3*
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Table S1. A comparison of clone libraries of 16S rRNA and hzo genes from the
mangrove sediments a
Homologous
library X site
P value for heterologous library Y site:
S0-s
S0-s
14
15
16
17
18
S1-s
S1-l
S2-s
S2-l
0.001 (0.001)
0.001 (0.093)
0.001 (0.035)
0.001 (0.019)
0.001 (0.001)
0.013 (0.011)
0.001 (0.001)
0.018 (0.001)
0.026 (0.001)
S1-s
0.001 (0.001)
S1-l
0.001(0.001)
0.042 (0.001)
S2-s
0.001 (0.001)
0.107 (0.204)
0.001 (0.245)
S2-l
0.001 (0.001)
0.233 (0.839)
0.415 (0.998)
0.001 (0.006)
0.077 (0.99)
a: In comparison between the homologous library X and the heterologous library Y,
boldface P values indicate that the libraries of 16S rRNA and hzo genes clone are
drawn from communities significantly difference (p<0.05). Numbers in parenthesis
refer to the P value of comparing hzo gene clone libraries and those without
parenthesis of 16S rRNA gene clone libraries.
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Fig. S1 Rarefaction analysis of anammox bacterial communities based on 16S rRNA (A) and
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hzo (B) gene sequences at the Mai Po Nature Reserve. DOTUR program was used with 2%
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nucleotide or protein sequence variation as OTU determination.
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S0-s
S1-s
S1-l
S2-s
S2-l
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Number of OTUs observed
6
5
4
3
2
1
0
0
5
10
15
20
25
30
35
Number of clones sequenced
23
24
25
A
2
14
S0-s
S1-s
12
S1-l
Number of OTUs observed
S2-s
10
S2-l
8
6
4
2
0
0
20
30
40
50
60
Number of clones sequenced
26
27
28
29
10
B
S-Fig. 1
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