Proforma for submitting proposal (2 copies) for organization of Training Programmes
under Centres of Advance Faculty Training in frontier and specialized areas of
agriculture and allied sciences (2013-2014)
1.
Topic of Training Programme:
“Updates on male infertility, semen technology and quality oocyte production”
2.
Justification of the proposed programme in the light of suggestive training needs in
the discipline (not more than 100 words):
Success of natural and artificial breeding program depends on quality of both
oocyte and semen. Poor libido, unacceptable semen quality and low freezability of semen
are the major issues in breeding dairy bulls which affects conception rates. A know how
of these factors requires evaluation of physical, endocrinological, seminal parameters of
bulls and freezability parameters of semen. Cryopreservation of semen also has undesired
effects on sperm, as it causes cryocapacitation and apoptosis of spermatozoa. Assessing
and minimizing these changes are crucial for increasing fertilization potential of sperms.
Quality of oocytes depends on hormonal environment during follicle development.
Understanding the effects of estrus synchronization and superstimulation protocols on
follicle development is essential for quality oocyte production. Our team of experts will
update the knowledge and provide training on the above mentioned protocols and
techniques that will help scientists in their related research programs.
3.
Venue with full postal/e-mail address and office phone/fax numbers:
Centre of Advanced Faculty Training in Veterinary Gynaecology and Reproduction
Department of Veterinary Gynaecology and Obstetrics
College of Veterinary Science
Guru Angad Dev Veterinary and Animal Sciences University
Ludhiana, Punjab 141004
E-mail address: parkashbrar@gmail.com and ajeetvet@yahoo.com
Office Phone: 0161-2400917, 2414003, Fax-0161-2400822
4.
Proposed dates (From – to):
September 16 to October 7, 2013
5.
Eligibility qualification for the participants of the Training Programme
Master’s in Veterinary Sciences (Scientist/faculty from department of Veterinary
Gynaecology and Obstetrics / Veterinary Physiology/ Livestock Production and
Management / Veterinary Biochemistry/ Animal Breeding and Genetics/ Dairy farm/
Animal Biotechnology).
ii) Working not below the rank of Assistant Professor and equivalent in the concerned
subject mentioned under Agricultural University /I.C.A.R. Institute.
i)
6.
Information regarding proposed Course Coordinator, if other than the Director,
CAFT, (enclose bio-data clearly bringing out the specific qualification, experience and
scientific contribution of the Course Coordinator in the proposed topic):
Dr Ajeet Kumar
(Brief bio-data enclosed)
Assistant Professor (Senior scale)
Department of Veterinary Gynaecology & Obstetrics,
College of Veterinary Science, GADVASU, Ludhiana 141004, Punjab
1
7.
Faculty Staff strength in CAFT (Assistant Professor, Associate Professor, Professor
and equivalent):
Assistant Professor: 7
8.
Associate Professor: 4
Professor: 5
Information regarding other academic staff of the host Institute who are likely to be
used as resource persons:
Scientists from Veterinary Microbiology, Livestock Production and Management,
Animal Breeding and Genetics, School of Biotechnology, School of public health and
zoonoses, GADVASU, Ludhiana are likely to be involved as resource persons.
9.
Specific facilities available for conducting the Programme such as laboratory
equipments/instruments, research farm, library, classroom, guesthouse etc.:
The department has well established semen laboratory, Breeding bulls and semen
collection facilities, Electroejaculator for semen collection, Fluorescent phase contrast
microscope, Zoom Stereo Microscopes, Electrophoresis and Western blotting
equipments, ELISA reader, RIA Lab for hormones, Ultrasound machines, Colour
Doppler, Ultrasound guided follicular aspiration/ ovum-pick up assembly, Instructional
herd of cattle (10) and buffaloes (10), well established library and classroom. One
guesthouse/ teachers home for accommodating participants for 21 days training is also
available.
10. Programmes/Projects and achievements in the area of special topic proposed for the
training programme:
Projects in the area of proposed topic:
a. Project entitled “Improvement in fertilizability of cryopreserved buffalo bull
semen by minimizing cryocapacitation and apoptosis like changes” funded by
Department of Biotechnology, Govt of India. (Ongoing).
b. Project entitled “Isolation and characterization of sperm specific antigenic
proteins with immunocontraceptive potential in dog” funded by Department of
Biotechnology, Govt of India. (Completed).
Achievements in the area of proposed topic:
a. Fertility prediction in buffalo bulls on the basis of sperm motion traits, fertility
associated antigens (FAA) and HSP70 expression.
Bull fertility depends upon the fertilizing ability of sperm. Variability in the results of
subjective evaluation of motility, live count and morphology poses challenge for
selection of high fertility bulls. Therefore, study was conducted to predict the fertility
of buffalo bulls using: a) CASA based sperm motion traits, b) Fertility associated
antigen (FAA) and c) expression for HSP70. Frozen semen from 20 buffalo bulls was
analyzed through CASA for Sperm motion traits. FAA was investigated in fresh
semen of the same bulls. For gene expression, total RNA was harvested, first strand
cDNA synthesized followed by quantification of HSP70 using a Real time PCR.
Primer and probe for HSP70 (Acc No U02892) and endogenous control (18S) were
designed using the Primer Express software package. Fertility trial was conducted on
166 adult normal cycling buffaloes. Bulls were categorized on the basis of fertility trial
based conception rates (CR) into good (CR>50%), average (CR 30-50%) and poor
(CR<30%) groups. In good fertility bulls, individual motility was higher (p<0.05) than
2
the poor fertility group. Progressive motility, VAP, VSL, VCL, ALH, BCF, STR and
LIN was similar (p>0.05) between the fertility groups. Out of 9 good fertility bulls, 7
were FAA positive. All the average and 3 poor fertility bulls were FAA positive. This
indicates that the assessment of FAA in buffalo bull semen did not indicate fertility.
Though, the expression for HSP70 in good, average and poor fertility buffalo bull
sperm were similar (p>0.05) between the groups, a specific trend exists with respect to
fertility. It is concluded that CASA based individual motility could be used for the
differentiation between good and poor fertility bulls.
b. Incidence and factors affecting libido in breeding buffalo bulls
Poor libido is a major problem in breeding bulls, which poses problem at the time of
natural and artificial breeding. The study involved 98 breeding buffalo bulls
maintained at different bull stations in Punjab to find out the incidence and associated
factors with poor libido. Bulls were categorized as good, poor and very poor libido
groups on the basis of reaction time. Almost one fourth (23.7%) of the breeding
buffalo bull population were affected with poor libido primarily associated with
lameness, scrotal dermatitis and or wide scrotal neck. Scrotal circumference, testicular
volume, semen volume and sperm concentration were similar (p>0.05) between very
poor, poor and good libido bulls. Individual sperm motility and livability were lower
(p<0.05) and abnormal sperm were higher (p<0.05) in very poor and poor libido bulls.
Wide variation in testosterone level (0.02 to 27 ng/ml) with respect to libido was
observed. The overall semen quality and freezability were also poor in very poor and
poor libido bulls.
c. Endocrinology and treatment of poor libido bulls
Poor libido is associated with poor semen quality and fertility ultimately affecting
financial profitability of the dairy farmers. Hence, there is need to develop a suitable
therapeutic strategy for the treatment of poor libido in breeding bulls. Eight crossbred
(Holstein Friesian x Sahiwal) breeding bulls with the history of gradual decline in
libido over a period of 4-6 months from an organized bull farm in Punjab were
selected. To rule out the possibility of physical stressful stimuli, which may affect
libido, breeding soundness evaluation of bulls was carried out. All the bulls were
given 3 injections of GnRH (Buserelin acetate) in decreasing dose i.e. 20µg, 16µg and
12µg at an interval of 2 days. Blood samples were collected before treatment and 24
hrs after every GnRH injection. Plasma Estradiol (E), Testosterone (T), T3 and T4
were analyzed using Microwell ELISA Kits. Breeding soundness evaluation based
observations did not reveal any defects in physical conformation, external or internal
genitalia indicating absence of physical stressful stimuli. The E to T ratio increased
from 6.57 to 44.28 in non responding bulls and decreased from 3.47 to 1.70 in
responding bulls indicating differential conversion of T to E following GnRH therapy.
Non responding poor libido bulls showed decline in T3 to T4 ratio from 90.0 to 64.54.
However, T3 to T4 ratio increased from 227.65 to 293.36 in treatment responding
bulls. It may be concluded that E to T and T3 to T4 ratios play an important role in
modulating the therapeutic response of GnRH. Three injections of GnRH (Receptal,
Buserelin) @ 20µg, 16µg and 12µg at an interval of 2 days improves libido in those
bulls which had lower E, higher T, T3 and T4 following the GnRH treatment.
d. Semen filtration: Improvement in semen quality and fertility of frozen semen
To improve post thaw semen quality, Sephadex (G-15) filtration of post thaw semen
was carried out. Post thaw unfiltered (control) and Sephadex filtered semen from four
3
bulls (three cross bred and one pure bred Holstein Friesian) were subjected to
microscopic examination viz. sperm concentration, individual motility, live sperm
count and sperm morphology. Sixty-two healthy, normal cycling crossbred cows were
inseminated with post thaw unfiltered (n=32) and filtered semen (n=30). Sephadex
filtration of post thaw semen decreased (p<0.05) total sperm concentration and sperm
with abnormal head, mid piece and tail. The overall average total sperm concentration,
head and tail defects in filtered semen decreased significantly (53.4, 1.2 and 6.4
million) than in the unfiltered semen (80.4, 2.4 and 15.7 million, respectively).
However, after filtration increase (p<0.05) in overall average motile and live sperm
concentration were observed (38.8 and 38.0) as compared to unfiltered semen (29.2
and 32.0 million, respectively). The overall conception rate recorded was 21.9% with
post thaw unfiltered semen and 56.7% with filtered semen. It was concluded that
Sephadex filtration of post thaw semen improved its quality and conception rate.
e. Pelvic Area and scrotal circumference in Poor Libido Breeding Bulls
Pelvic area is a highly heritable trait which increases the pelvic size of female
offspring upon selection thereby decreasing the incidence of foeto-pelvic
disproportion. Study was conducted to assess pelvic area, scrotal circumference in
relation to libido in bulls. Twelve bulls with poor libido and seven bulls with good
libido were selected on the basis of reaction time. Breeding soundness evaluation was
done to access the internal pelvic area and scrotal circumference. The internal pelvic
area was calculated by the Arlong’s formula. The average internal pelvic area in poor
libido bulls was significantly lower (416.82  62.41 cm2) than in the good libido bulls
(748.16  26.56 cm2). However, the scrotal circumference was similar 31.81  1.03
cm in poor libido bulls as compared to 35.14  0.90 cm in good libido bulls. The
correlation coefficient between the internal pelvic area and scrotal circumference in
poor and good libido bulls was -0.445 and +0.879, respectively. It was concluded that
internal pelvic area is significantly lower and has negative correlation with scrotal
circumference in poor libido bulls.
f. Embryo production and endocrine changes in superovulated buffaloes.
Pre-implantation murine and bovine embryos obtained after suitable superovulatory
regimen and flushing were assessed for viability by morphological, dye exclusion,
sucrose induced shrinkage, rolling and in vitro culture techniques. 460 murine and 79
bovine embryos judged morphologically revealed 71.5 and 69.6 per cent viable
respectively. Morphologically evaluated viable embryos were subjected to dye
exclusion assay using Eosin-Y, Eosin-B, Trypan Blue and Rose Bengal Red indicating
89.4, 89.5, 85.0 and 91.3 % viable embryos correspondingly. The developmental
competence of unstained embryos were 72.6, 49.4, 80.4 and 75.3 % correspondingly.
The most suitable dyes with best concentrations in increasing order evaluated at P <
0.05 were EosinB (0.12 mM) < Rose Bengal Red (0. 5mM) < Eosin-Y (0.12 mM) <
Trypan Blue (0.05%). The results obtained for bovine embryos at these dye
concentrations were 66.6, 72.7, 72.7 and 72.2 % with developmental competence of
80, 87.5, 75.0 and 61.5 % correspondingly. Sucrose induced shrinkage of embryos
indicated 71.1 per cent out of 273 murine embryos and 65.5 per cent out of 55 bovine
embryos as viable. Rolling of embryos in culture medium revealed 70.7 % of 273
murine and 63.6 % of 55 bovine embryos as viable. In vitro culture of preimplantation embryos indicated 73.6 and 70.9 per cent viable murine and bovine
embryos respectively. Significant differences (P<0.05) between morphological
evaluation and other methods was evident, but no significant difference between dye
4
exclusion assay and developmental competence of embryos in culture media was
observed. Thus, it was concluded that dye exclusion assay had an advantage over other
viability assays of pre-implantation bovine and murine embryos.
g. Factors effecting in vitro maturation of oocytes in buffaloes.
Using a prospective comparative design, in vitro maturation of buffalo oocytes, vis-avis culture treatments, with three basal culture media - Ham's F-10, Ham's F-12 and
TCM-199; supplemented with estrus sera-buffalo (BES) and cow estrus serum (CES),
and, co-culture cells-granulosa (GC), cumulus (CC) and oviductal epithelial cells
(OEC), were evaluated. A total of 3335 cumulus-oocyte-complexes were aspirated
from 2952 buffalo ovaries, procured from a slaughterhouse. COCs were matured in
vitro at 38.5°C in 5 % CO2 and 95 % RH upto 30h. TCM-199 fortified with FCS,
CES, pyruvate, FSH and LH was used as IVM medium to study chronology of oocyte
maturation. Maximum maturation rates of 52.6, 55.6, 74.2, 66.3, 73.5, 70.9, 60.4 and
68.4 per cent were obtained in TCM-199 with zero, BES, CES, BES+CES, GC, CC,
OEC and FSH+LH supplementation, respectively at 28h of IVM. For Ham's F-10 and
Ham's F-12 media the corresponding IVM rates were 52.8, 48.7, 69.5, 59.0, 69.0, 68.3,
54.7 and 62.7, and, 55.9, 52.2, 70.9, 61.3,71.1,71.5,52.6 and 60.8 per cent,
respectively. IVM rates for TCM-199 were significantly higher (P<0.05) than for
Ham's F-10 and Ham's F-12 except in control and CC supplementation. Ham's F-12
resulted in higher IV1vl rates than Ham's F-10 but the differences were nonsignificant. CES supplementation led to significantly higher (P<0.05) IVM rates than
BES or BES+CES Higher IV M rates with GC than CC supplements were obtained
but the differences were non-significant. OEC supplement led to significantly lower
IVM rates than GC or CC supplements. 85.6 per cent of buffalo oocytes were at
GVBD stage by 6h and 67.7 per cent at metaphase-Il stage by 24h of IVM. The results
indicated that TCM-199 with CES, GC or CC supplements was best for IVM and
GVBD and metaphase-II stages occurred at 6 and 24h of start of IVM, respectively,
for buffalo oocytes. The use of cow estrus serum and co-culture cells for IVM of
buffalo oocytcs has been investigated for the first time.
11. Schedule of daily lectures/practical topics to be covered and name of the faculty
proposed to be engaged during the CAFT Training Programme:
Sl.
No.
1
Date
/Day
16.09.13
(Mon)
2
3.
4.
5.
6.
17.09.13
(Tue)
Time
Topic of lecture/Practical
Name & Designation of
Speaker
9.30 11.30 am
11.301.00 pm
2.003.00pm
3.30-5.00
pm
9.3010.30am
11.0012.00 am
Registration, Introduction of participants and
faculty members
Visit to different wings of the department &
Teaching veterinary hospital
Breeding soundness evaluation: a tool to Dr Ajeet Kumar
identify fertile bulls
Assistant Professor
Endocrinology of males
Dr SPS Ghuman
Associate Professor
Basics of ultrasonography in animal Dr M Honparkhe
reproduction
Assistant Professor
Ultrasonography of males to identify diseases Dr Ajeet Kumar
affecting fertililty
Assistant Professor
5
7.
8.
9.
18.09.13
(Wed)
10.
11.
12.
13.
14.
19.09.13
(Thu)
20.09.13
(Fri)
15.
16.
17.
20.
21.09.13
(Sat)
23.09.13
(Mon)
21
22
23
24.09.13
(Tue)
24
20
21
25.09.13
(Wed)
22
23
24.
26.09.13
(Thu)
12.001.00 pm
2.00-5.00
pm
9.3010.30am
11.0012.00 am
Managemental
techniques
to
optimize Dr Chandrahas
reproductive efficiency in males
Assistant Professor
Breeding soundness evaluation of bulls
Dr Ajeet Kumar
12.001.00 pm
2.00-5.00
pm
9.3010.30am
11.0012.00 am
12.001.00 pm
Role of seminal plasma proteins in semen
freezability and fertility
Semen
collection
from
bull
using
electroejaculator
Cryopreservation of bull semen
2.00-5.00
pm
9.3011.00am
11.301.00 pm
2.00-5.00
pm
10.301.30pm
9.3011.30 am
12.001.00pm
2.005.00pm
9.3011.00am
11.301.00pm
2.00-5.00
pm
9.3011.00 am
11.301.00pm
2.00-5.00
pm
9.3011.00
Demonstration of semen collection and AI in
canines
Impact of environmental toxicity on semen
quality and fertility.
Use of various additives, cryoprotectants,
sugars on seminal parameters.
Semen collection, evaluation and AI of buck
semen
Hands on training of breeding soundness
evaluation and ultrasonography
Semen evaluation: traditional and newer
approaches
Semen collection, seasonal variations in semen
quality and fertility in equines
Semen filtration through various techniques
Dr Ajeet Kumar
Update on status of bull fertility and semen
technology in India
Cryoprotectants and cryoinjury: structural and
molecular aspects
Cryopreservation of semen in equine, boar,
goat
Status of semen technology and AI in canines
Assistant Professor
Dr PS Mavi
Associate Professor
Dr
M.
Anzar*,
Professor, Univ of
Saskatchewan, Canada
Dr AK Singh
Asstt Professor
Dr Ajeet Kumar
Assistant Professor
Dr R. Cheema
Associate Professor
Dr VK Gandotra
Professor
Dr Sarita Gulabne*
Associate Professor
Vety College Bombay
Dr Sarita Gulabne*
Associate Professor
Dr J S Bedi
Assistant Professor
Dr A K Singh
Assistant Professor
Dr Chandrahas
Assistant Professor
AK Singh
Asstt Professor
Dr SPS Ghuman
Associate Professor
Dr PS Brar
Professor
Asstt Professor
Semen collection, semen quality and fertility in Dr VK Gandotra
boar and small ruminants
Professor
Use of herbal libido enhancer drugs in bulls
Dr VK Singla
Assistant Professor
A K Singh
Assistant Profssor
Dr G Umapati*
CCMB, Hyderabad
Dr CS Mukhopadhay
Assistant Professor
Maninder Kaur/
Dr R S Cheema
Newer tools to study fertility biomarkers: Dr J Jagannathan* Prof,
Cryopreservation of semen at ultra low
temperature
Semen collection, semen quality and fertility in
wild and captive animals
Potential biomarkers to identify high fertility
bulls
Harvesting of sperm membrane proteins
nuclear magnetic resonance
6
AIIMS, N Delhi
11.301.00 pm
2.00-3.00
pm
3.30-5.00
pm
9.3011.00
11.30
am-1.00
pm
2.00-3.00
pm
Quality control measures to produce semen for Dr Ranjana Cheema
export
Assoc Professor
Poor libido in males: etiology and management Dr Ajeet Kumar
3.305.00pm
29.
28.09.13 10.30(Sat)
1.00 pm
32.
30.09.13 9.30(Mon)
11.30 am
33.
12.001.00 pm
34.
2.00-5.00
pm
35.
01.10.13 9.30(Tue)
10.30 am
36.
11.001.00 pm
2.00-5.00
pm
37.
03.10.13 9.00(Thu)
11.15 am
38.
11.301.00 pm
39.
2.00-5.00
pm
40.
04.10.13 9.30(Fri)
11.00 am
41.
11.305.00pm
42
05.10.13 9.30(Sat)
11.00am
07.10.13 9.30(Mon)
11.00 am
11.001.00 pm
2.00-5.00
pm
*Proposed outside experts
Dr Amrit K Bansal/
Dr R S Cheema
Dr. D Dadarwal
Asstt Professor
Dr PS Brar
Professor
Dr D Dadarwal
Assistant Professor
Ajeet Kumar
Asstt Professor
Dr D Dadarwal
Assistant Professor
Dr Narinder Singh
Assistant Professor
Dr Ajeet Kumar
Asstt Professor
Dr AK Arora
Professor
Dr D Dadarwal
Asstt Professor
Dr M Honparkhe
Asstt Professor
Dr S Prabhakar
Professor
Dr D Dadarwal
Assistant Professor
Dr Narinder Singh
Assistant Professor
Dr Prahlad Singh
Assoiciate Professor
Dr R S Cheema
Associate Professor
25.
26.
27.
28.
27.09.13
(Fri)
2D gel electrophoresis to identify sperm
membrane proteins
Problems and measures to improve AI services
under field conditions
Diseases transmitted through semen and
vaccination in bulls, equines and small
ruminants
Lipid peroxidation mediated loss of sperm
motility and its modulation through various
additives
Western blotting to identify sperm membrane
proteins
Statistical analysis of data on animal
reproduction
Factors affecting superstimulation in cattle and
buffaloes
Defining oocyte Nuclear maturation :
comparison across species
Assessment of membrane integrity and
migration capability : HOST, CMPT
Factors affecting oocyte quality
How to assess that oocyte is happy?
Immuno-histochemistry of sperm
Microbial quality control for semen samples
Defining oocyte cytoplasmaic maturation :
comparison across species
Transvaginal ultrasound guided ovum-pick-up
technique for COC collection
Synchronization of estrous in dairy cattle and
buffalo
Follicle aspiration from slaughter house ovaries
and morphological evaluation of COC
In vitro maturation bovine oocyte and
assessment of maturation
Production of sexed semen and embryo and its
applicability
Male contraceptives to control stray population
in dogs
Valedictory function
7
Assistant Professor
Dr AK Bansal/ Dr
Ranjana Cheema
Dr GS Dhaliwal
Professor
Dr AK Arora
Professor
AK Singh /
Dr Amrit K Bansal
12. Name of the Trainings organized during the last three years:
S.
No.
1
2
3
Name of the training
Dates
Advances in applications of diagnostic techniques in veterinary 2010-11
theriogenology
Prevention and therapeutic management of peri-parturient complications in 2011-12
domestic animals
Application of diagnostic ultrasound techniques in animal reproduction
2012-13
13. Signature of the Director of the CAFTs (With official Seal):
8
BRIEF BIODATA OF COURSE COORDINATOR
Name & Designation:
Field of specialization:
AJEET KUMAR
Assistant Professor (Senior scale)
Department of Veterinary Gynaecology & Obstetrics,
College of Veterinary Science, GADVSU,
Ludhiana, Punjab, India
Veterinary Gynaecology and Obstetrics
Professional Experience:
 Teaching, research and extension experience of 10 years.
 Performing Bull evaluation, infertility diagnosis, semen collection, evaluation since
1999.
 Master’s and PhD programme were exclusively on the Bull fertility / semen
technology:
MVSc- "Effect of Sephadex filtration of post thaw bull semen on fertility".
PhD- “Studies on gene expression for heat shock protein 70 and identification of
sperm membrane proteins in relation to semen quality and fertility in buffalo
bulls”
Specific qualification:
 Attended six months international training program entitled “Identification of high
fertility bulls at an early age using metabolic fingerprinting” at Department of
Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada
during May to Oct, 2012.
 Carried out collaborative research entitled “Identification and characterization of
Heat Shock Proteins (HSP70) in sperm membrane” at Department of Veterinary
Biomedical Sciences, University of Saskatchewan, Canada under Canadian
Commonwealth Fellowship (2009) by Canadian Bureau of International Education
(CBIE).
Scientific contribution in proposed topic:
 Research Project as Principal Investigator (PI) - “Improvement in fertilizability of
cryopreserved buffalo bull semen by minimizing cryocapacitation and apoptosis like
changes” funded by Department of Biotechnology, Govt. of India, New Delhi.
 Collaboration in other projects in the department:
- Investigations on poor libido in breeding bulls
- Infertility problems in bulls
- Measures to improve semen quality and fertility
 Published 2 international and 10 national research papers exclusively on male fertility
or semen technology.
Professional recognition:
 Travel Award for Young Scientists from developing countries (2012) in
International Congress on Animal Reproduction (ICAR) at Vancouver, British
Columbia, Canada.
9





Canadian Commonwealth Fellowship (2009) by Canadian Bureau of International
Education to carry out research at Department of Veterinary Biomedical Sciences,
University of Saskatchewan, Canada.
Professor Nil’s Lagerlof Award (2005) for best research work on animal
reproduction, bestowed by the Indian Society for Study of Animal Reproduction
(ISSAR).
Best Young Scientist Award (2001) for best scientific paper presentation, bestowed
by the Indian Society for Study of Animal Reproduction (ISSAR).
University Merit Scholarship (1994-1996) during B.V.Sc & A.H program.
State Talent Rural Scholarship (1985-1989) from Department of Primary Education,
Govt. of Bihar, India.
10