PATTERNS IN NATURE Outline the historical development of the cell theory, in particular, the contributions of Robert Hooke and Robert Brown. Things to consider: - What does outline mean? - Underline key words. - Understand what the question is asking before you write your answer. Their have been many scientific contributions towards the development of the cell theory. Of importance were the contributions of Robert Hooke. Robert Hooke began to use the compound microscope in 1665. The compound microscope had already been invented by Hans and Zacharias Janssen in 1590. It was in 1665 that Hooke observed the first cells. He did this by observing thin slices of cork and describing the boxes filled with air as cells. Robert Brown also made significant contributions towards cell theory. Brown identified and described the nucleus of a plant cell in 1827. These contributions are significant in the development of cell theory as it enabled scientists to further research and develop techniques to prove that all organisms are composed of cells. Describe evidence to support the cell theory. Things to consider: - What does describe mean? - Creative way to present answer??? - Be succinct. SUPPORTING THE CELL THEORY EVIDENCE DESCRIPTION 1665 Discovery cells in cork. Robert Hooke used a compound microscope to describe little boxes of air, which in turn he called cells. 1676 Discovery of microorganisms. 1827 Discovery of nucleus in plants. 1838 Discovery of cell replication. 1879 Proof of cells dividing. Leeuwenhoek identifies microorganisms in pond water which he views using a microscope. Robert Brown discovers and describes the nucleus in plant cells. Schwann views single yeast cells budding and producing new cells. This leads to the conclusion that all cells are the building blocks of life and subsequently mitosis. Flemming uses a biological stain to view cells. He discovers that cells divide supporting Virchow’s hypothesis. The above evidence supports cell theory as it shows the gradual steps as to the final conclusion that all living things contain cells, which in turn replicate. (Mitosis) Use available evidence to assess the impact of technology, including the development of the microscope on the development of the cell theory. Things to consider: - What does assess mean? - What does impact mean? - Relate the development of the microscope to cell theory Technology has had a huge effect on the development of cell theory. In particular was the development of the light microscope. The light microscope was developed and refined over hundreds of years from the initial stages where Italian monks invented glass magnifying spectacles, to that of the current day light compound microscope. It was through the invention of the light microscope and the work of various scientists including Hooke, Brown, Schwann and Flemming that the hypothesis that all organisms contained cells was proven, and hence cell theory was established. Therefore by the work of Hooke discovering cells using the microscope, Brown identifying and describing the nucleus using a microscope, Schwann witnessing yeast budding and producing new cells using a microscope and Flemming using biological stains to view mitosis using a microscope further supports cell theory as well as how technology has affected the development of cell theory. Discuss the significance of technological advances to developments in the cell theory. Things to consider: - What does discuss mean? - Underline key words - Be succinct There have been many technological advancements which have led to further developments in cell theory. In chronological order they are: TECHNOLOGICAL ADVANCE 1: The development of the light microscope: The light microscope was developed over hundreds of years. It enabled Hooke to identify the first cells, which in turn led to further discoveries by Brown, Schwann and Flemming. Therefore establishing and proving cell theory. TECHNOLOGICAL ADVANCE 2: The use of a biological stain: The use of the biological stain has enabled scientists to make further discoveries about cells and their function. Biological stains stain certain parts of the cell and highlight their structure. It was the work of Flemming who first used a stain in 1879 to view cells dividing that further supports cell theory. TECHNOLOGICAL ADVANCE 3: The use of oil immersion: Oil immersion was developed so that scientists could view extremely small cells such as bacteria. Through oil immersion cell theory has been further supported. TECHNOLOGICAL ADVANCE 4: The development of the electron microscope: The electron microscope was developed due to the low magnification and clarity of the light microscope. The electron microscope was fully developed in 1939 and has enabled scientists to view cells in more detail. This therefore supports and proves cell theory. Identify cell organelles seen with current light and electron microscopes. Things to consider: - What does identify mean? - Be succinct The cell organelles which can be seen with the current light microscope are; chloroplasts, nucleus and vacuoles. The cell organelles that can be seen with the current electron microscope are; nucleus, chloroplasts (plastids), vacuoles, mitochondria, golgi body (apparatus) lysosomes, ribosomes and the endoplasmic reticulum. Describe the relationship between the structure of cell organelles and their function. Things to consider: - What does describe mean? - Think of a creative way to formulate an answer…..table?? ORGANELLE NUCLEUS STRUCTURE/FUNCTION The nucleus is a dark large spherical structure which contains DNA and RNA. The DNA in particular is responsible for cell structure and chemical activity within the cell. PLASTIDS The plastids are a group of organelles which include chloroplasts, chromoplasts and leucoplasts. Chloroplasts are cylindrical in structure and contain grana. The grana contain chlorophyll which is responsible for photosynthesis. Chromoplasts contain colours for fruit and flowers. Leucoplasts store starch. MITOCHONDRIA Mitochondria are sausage shape structures which contain tiny membranes called cristae. The angle of the cristae enables a larger surface area which in turn increases surface area. The main function of mitochondria is cellular respiration and the production of ATP. ENDOPLASMIC ER is a series of flattened sacs, tubules and membranes. The RETICULUM cells chemical activity takes part in the ER as well as the transport of nutrients and the removal of wastes. RIBOSOMES Ribosomes are found all throughout cells They are seen as tiny black dots. Their main function is protein synthesis. GOLGI BODIES Specialised areas of ER. Semi circle arrangement of membranes. On the end of theses membranes are tiny vesicles. These vesicles transport substances to the cell membrane. Other functions include the sorting of proteins. (Transport within the cell.) LYSOSOMES Tiny vesicles (sacs) which destroy any foreign material entering the cell. VACUOLES Large fluid filled sacs which contain water, salts and other molecules. Provides support for the cell. Perform a first-hand investigation to gather first-hand information using a light microscope to observe cells in plants and animals and identify nucleus, cytoplasm, cell wall, chloroplast and vacuoles. Things to consider: - What does perform mean? - Underline key words. - Ensure you know how to use a microscope (refer to class notes) - Ensure you know how to prepare a wet mount (refer to class notes) - Ensure you know how to prepare a stained slide (refer to class notes) REFER TO PAGE 107 – 108 OF THE SCIENCE TEXT BOOK. Process information from secondary sources to analyse electron micrographs of cells and identify mitochondria, chloroplasts, Golgi bodies, lysosomes, endoplasmic reticulum, ribosomes, nucleus, nucleolus and cell membranes. Things to consider: - What are electron micrographs? - What does identify mean? - Ensure you obtain/observe a micrograph with all of the above stated organelles. MITOCHONDRIA CHLOROPLAST GOLGI BODY AND LYSOSOMES Identify that there is movement of molecules into and out of cells. There is movement of molecules in and out of cells. However this is restricted by the cell membrane also known as the phospholipid bilayer. This layer is selectively permeable, which means it only allows certain molecules in and out of the cell. Describe the current model of membrane structure and explain how it accounts for the movement of some substances into and out of cells. Things to consider: - Describe means to provide characteristics or features of. - Explain means to relate cause and effect - Firstly give features of the fluid mosaic model structure. - Then explain how these features account for the movement of some substances into and out of cells. The main structures of the fluid mosaic model include glycolipids, cholesterol, carbohydrates, glycoproteins, phospholipids and proteins. These structures in turn all have a specialised function. These functions account for the movement of some substances into and out of cells. - The phospholipid bilayer acts as a barrier to ions, polar substances and large molecules. Water is an exception because it easily travels through the bilayer. - The proteins allow substances such as amino acids to pass through the membrane. Their pore allows substances to pass through the membrane. - The carbohydrate molecule projects towards the extracellular fluid. Its main role is to reject foreign cells and molecules away from the cell. It is also expected to accept cells or molecules recognised as its own. This recognition phase is mainly undertaken by the glycoprotein. Identify that there is movement of molecules into and out of cells. Things to consider: - Identify means to recognise and name - This question is a low order question meaning it will only require a short answer There is movement of molecules into and out of cells. Molecules move into and out of cells by active or passive transport. This process of transport is related to diffusion. Diffusion is the movement of a substance from where it is more concentrated to an area of less concentration. Perform a first-hand investigation to model the selectively permeable nature of a cell membrane. OBSERVING DIFFUSION AIM: To observe and describe an example of diffusion. MATERIALS: 2 Beakers Straw Potassium Permanganate METHOD: 1. Label one beaker “cold” and the other “hot,” then three-quarters fill each with cold and hot water, as labelled. Put the beakers where they can be left undisturbed. Wait five minutes to let the contents settle. 2. Using the straw, carefully position a crystal at the bottom of each beaker as shown below. Try not to disturb the water as the straw is withdrawn. RESULTS: Draw a diagram of each beaker and use shading to show the differences in colour intensity of the solutions for: - the changes you observe over half an hour in both beakers - the changes you observe in the next lesson Compare the processes of diffusion and osmosis. Things to consider: - What does compare mean? - What is the best way to present our data? - The best way to present our data would most likely be in the form of a table. DIFFUSION The process by which substances move from an area of high concentration to an area of low concentration. The difference in concentration between two areas is usually due to the fact of a barrier. This barrier is known as the concentration gradient. No cellular energy is needed. The energy within the molecules is suffice for the molecules to move from either side of the concentration gradient. An example of diffusion is carbon dioxide leaving the blood stream and into the lungs. OSMOSIS Osmosis is the process of water crossing a semipermeable membrane. This means that the difference between diffusion and osmosis is that osmosis is concerned with water crossing the gradient and diffusion is concerned with molecules crossing the gradient. Compare prokaryotic cells to eukaryotic cells. PROCARYOTIC Earliest type of cells Do not contain a nucleus nor any membrane bound organelles. All substances for cell function are found in the cytoplasm. EUCARYOTIC Contain a nucleus and membrane enclosed organelles Multicellular Examples include plant and animal cells. Unicellular Contain only ribosomes. Examples include bacteria. OBSERVING OSMOSIS AIM: To model the function of a cell membrane METHOD: 1. Three-quarters fill the gas jar with water and add iodine/potassium iodide solution until the water is dark brown. 2. Moisten the dialysis tubing and tie a very tight knot in one end. Insert the stem of the thistle funnel into the other end and fasten it firmly with the rubber band. 3. Pour starch solution through the thistle funnel into the dialysis bag until the bag is full and the starch level is 2-3cm up the stem of the funnel. Make sure that there are no air bubbles trapped in the bag. Rinse the outside of the bag and funnel with water to remove any traces of starch. 4. Mark the level of starch on the funnel stem and then suspend the apparatus as shown in Figure 2. Let the apparatus stand undisturbed for 30 to 60 minutes. RESULTS: Draw large and accurate ‘before’ and ‘after’ diagrams of your apparatus. Note the liquid level in the funnel stem. Label the diagrams fully. DISCUSSION: 1. From your results, what is the evidence that some of these molecules have moved through the dialysis bag? On your diagrams indicate in which direction there has been a net (overall) movement of each type of molecule. 2. Suggest a reason why some molecules have moved through the dialysis bag while others have not. 3. Is there any evidence that there has been a net movement of water into the dialysis bag? Explain how you decided whether such a movement occurred. 4. Based on your observations, and referring to your text book if necessary, write down a definition of osmosis. CONCLUSION: This experiment modelled the movement of water in living cells. In your conclusion consider the following: - What did the dialysis bag represent in this model? - What did the starch solution represent? - From your observations, explain how water enters living cells. Discussion answers: 1. Evidence should include: - Change of colour. (Black = starch and iodine have reacted) - The volume of the dialysis bag - The volume in the gas jar 2. Main reason would be due to the fact that the dialysis bag is selectively permeable to certain substances. E.G. larger molecules would not be able to permeate through the bag 3. Evidence to suggest that there has been some net movement of water into the bag would include the volume in the bag as well as the volume in the gas jar. (Water molecules attached to iodine molecules) 4. Osmosis is the diffusion of water across a semi-permeable membrane Explain how the surface area to volume ratio affects the rate of movement of substances into and out of cells Things to consider: - What does “explain” mean? - What is surface area to volume ratio? - How does this affect the movement of substances in and out of cells? Firstly the surface area to volume ratio is the amount of surface area of an object compared to its apparent volume. This definition can be linked to cells and how surface area to volume ratio affects the rate of movement of substances into and out of cells. Cells obtain nutrients and release waste through their membrane through diffusion. This covers the whole surface area of the cell. The cell carries out important chemical reactions (metabolism) within the volume of the cell. It is important that a cell has a large surface area to volume ratio because then there is enough surface area to supply the volume with nutrients as well as the diffusion of wastes out of the cell to keep the cell in working order and alive. Perform a first-hand investigation to demonstrate the effect of surface area to volume ratio on rate of diffusion AIM: To investigate the relationship between the surface area to volume ratio of an object, the rate of diffusion of a substance into it, and the relationship between surface area and rate of reaction. METHOD: 1. Accurately cut the jelly sheets into blocks as indicated by your table. 2. Half fill the beaker with sulphuric acid. 3. Add the blocks to the beaker and gently stir. Watch until the first block goes completely clear then proceed to the next step immediately 4. Remove all the blocks from the acid. Rinse in water and pat them dry. Working quickly cut them in half and measure in millimetres the depth of the clear layer in each. 5. Record results in table. RESULTS: BLOCK NUMBER 1 2 3 Block Dimensions (mm) 20 x 20 x 20 20 x 20 x 10 20 x 20 x 5 3 2 1 Order of clearing Depth of clear part of block (mm) Student answers Student answers Student answers Surface area (mm2) 2400 1600 1200 Volume (mm3) 8000 4000 2000 Surface area to volume ratio (SA÷V) 0.3:1 0.4:1 0.6:1 QUESTIONS: 1. What causes the blocks to become clear? 2. Which block was first to go clear 3. From your observations of the other two blocks, predict which would be next to go clear. Explain your prediction. 4. Which block in your table had the largest surface area to volume ratio? 5. Does the size of a block influence the rate at which acid diffuses into it? Refer to surface area and volume in your answer. ANSWERS: 1. A chemical reaction occurs whereby the sulphuric acid diffuses into the jelly. This in turn reacts with the sodium hydroxide and the phenolphthalein to turn the block clear. 2. Block number three 3. Block number two. This is due to the fact that it has a greater surface area to volume ratio to that of block one, which in turn means block two would have a greater reaction rate. 4. Block number three had the largest surface area to volume ratio 5. The size of the block does influence its rate of diffusion. This is evident in the results. The results showed that block three which had the largest surface area to volume ratio obviously had the quickest diffusion as the block went clear the quickest out of the two. Identify some examples that demonstrate the structural and functional relationships between cells, tissues, organs and organ systems in multicellular organisms. Brainstorm activity. Things to consider: - What does identify mean? - What is the question asking? - Can we think of any examples? THE EXAMPLE WE USED WAS WRITTEN IN CLASS. (CARDIOVASCULAR SYSTEM) Distinguish between autotrophs and heterotrophs in terms of nutrient requirements. Things to consider: - What does distinguish mean? - What are autotrophs and heterotrophs? - Nutrient requirements: Nutrients they need to survive. Autotrophs are organisms that make their own food. They make their food by trapping energy from another system. For example plants make their own food in the process of photosynthesis. Bacteria on ocean floors make their own food through chemosynthesis. Autotrophs are different to that of heterotrophs because heterotrophs can not make their own food. Heterotrophs have to rely on other organisms to make their food. For example humans eat meat. Identify the materials required for photosynthesis and its role in ecosystems. Things to consider: - What does identify mean? - Name the materials required - How does this help ecosystems? The materials required for photosynthesis include: - Water - Carbon Dioxide - Sun Light - chloroplasts The role of photosynthesis in ecosystems is to produce energy and food for plants to survive; this in turn can feed other heterotrophic organisms. E.G. Cows eating grass. Identify the general word equation for photosynthesis and outline this as a summary of a chain of biochemical reactions. Things to consider: - What is the meaning of identify? - The word equation for photosynthesis - What does outline mean? - What are biochemical reactions? The word equation for photosynthesis can be seen on page Firstly the term biochemical refers to the chemistry of living matter. In this case we are talking about plants and how they undergo photosynthesis. For photosynthesis to occur plants need the following, carbon dioxide, water, sun light and chlorophyll. The chlorophyll “traps” the sunlight in the plant cells; this then reacts with the two reactants carbon dioxide and water. Once this reaction has taken place a product of oxygen and glucose has been produced. Plan, choose equipment or resources and perform first-hand investigations to gather information and use available evidence to demonstrate the need for chlorophyll and light in photosynthesis As a class we need to design an experiment which will address the following questions: Is chlorophyll necessary for starch production? Is light necessary for starch production? Things to consider: - What results can be expected? - How will the actual results be recorded? - Are any controls needed? - Are their any problems with the design PART A: AIM: To determine if chlorophyll and light are necessary for starch production. EQUIPMENT (Apparatus) Plants Dark cupboard Sunlight Iodine solution 100ml beaker METHOD: 1. Water both plants with 100ml of water 2. Put one plant into a dark cupboard for the weekend 3. Place the other plant in direct sunlight for the weekend. 4. Record any observations you see in the plants. RESULTS: Describe any changes between the two plants. PART B: Testing a leaf for starch METHOD: 1. Using a Bunsen burner bring 75ml of water to the boil. Then turn off the Bunsen. 2. Place leaves to be tested into the water. When the leaves are limp remove them with forceps and place in evaporating dish. 3. Using the forceps put the softened leaves into a test tube of alcohol. 4. Place this test tube into the beaker of water and place on the hot plate. The alcohol should start to turn green. 5. Remove the leaves when they are pale and place them into an evaporating dish. 6. Rinse the leaves in the hot water that is still in your beaker and place them in an evaporating dish. 7. Cover each leaf with iodine/potassium iodide solution and allow them to stand for a minute. RESULTS 1. Write an account of what you observed during this experiment. 2. Compare the two sets of results. Were there any differences? If so what were they? 3. State why it was safer to burn the alcohol on the hot plate 170 4. Rather then the Bunsen burner. 5. Why are chlorophyll and light required for photosynthesis to occur? CONCLUSION From your investigations, are chlorophyll and light necessary for starch formation? Explain the relationship between the organisation of the structures used to obtain water and minerals in a range of plants and the need to increase the surface area available for absorption. Things to consider: - What does explain mean? - Simplify the question. For example know what the question is asking before writing an answer. - Underline key words - Remember to use scientific terms. - Refer back to question Plants are simple in their nutritional requirements. They only require water and minerals. Water enters the plant by osmosis while minerals are mainly absorbed through active transport. There are many different groups of plants which in turn means there are many different ways to absorb water and nutrients. Firstly there are monocot plants. These plants have a fibrous root network. These fibrous roots increase the surface area for the absorption of water and mineral ions. The second group of plants are dicotyledons. This group of plants have a main tap root as well as roots that branch off. This system in turn increases the surface area available leading to greater water and mineral ion absorption. The third group of plants is a symbiotic relationship between a fungus and the plant. Mycorrhizae consist of a large network of fungal threads which surround the root hairs of plants. Water and minerals are absorbed through the fungal cells as well as the plant roots. The plant receives water from the mycorrhizae in return for nutrients. This symbiotic relationship increases the surface area for the absorption of water and minerals. Explain the relationship between the shape of leaves, the distribution of tissues in them and their role. Things to consider: - What does explain mean? - Simplify the question so you understand what the question is asking. - Underline key words - Remember to use scientific terms - Refer back to the question The leaves of many plants are flat and thin, this increases the surface area for light absorption. Palisade mesophyll cells are packed just below the upper cuticle of the leaf. These cells are packed with chloroplasts which enhances photosynthesis. Next in line are the spongy mesophyll cells. These cells are surrounded by air spaces filled with carbon dioxide and oxygen. Gas is exchanged in the leaves through tiny holes called stomates. Therefore the shape of the leaf as well as photosynthetic leaf cells maximise the surface area for photosynthesis to occur. The chloroplasts also play an important role in increasing surface area for photosynthesis. They are a large organelle structure which contain thylakoids and granum. Thylakoids contain large amounts of chlorophyll which traps the sunlight. The thylakoids are stacked upon one another and are called granum (grana). The light reactions of photosynthesis take place in the grana. The arrangement of the grana increases the surface area and hence increases the efficiency of photosynthesis. AIM: To determine if the surface area of calcium carbonate will affect the rate of reaction. EQUIPMENT: Marble chips, calcium carbonate powder, dilute HCl, test tubes with stoppers and side arms, test tubes, lime water, 10ml measuring cylinder, electronic balance and test tube rack. METHOD: 1. Pour 10mL of HCl into 2 test tubes with side arms and place them in the test tube rack. 2. Pour 10mL of lime water into 2 plain test tubes and place them in the test tube rack. 3. Collect a marble chip. Weigh the marble chip and record its weight. 4. Collect some calcium carbonate powder. Weigh the calcium carbonate and make sure it is the same weight as the marble chip. 5. Connect your test tubes as indicated below. 6. At the same time place both the powder and marble chip into their respective HCl test tubes. 7. Observe the two tubes and the reactions taking place. 8. The lime water should turn a murky colour indicating the presence of carbon dioxide and a reaction taking place 9. Record which substance reacted the fastest. RESULTS Weight of marble chip: _______________ Weight of calcium carbonate powder: ____________________ Observations: Describe the role of teeth in increasing the surface area of complex foods for exposure to digestive chemicals Things to consider: What does describe mean? Simplify the question Underline key words Be succinct Refer back to the question The role of teeth in mammals is to break down food and to increase the surface area of this food so that enzymes can break down this food. The human mouth consists of four types of teeth incisors, canines, pre-molars and molars. There are a total of eight incisors in the human mouth. Their specialised function is cutting and biting. Canines are situated next to the incisors. They are used for ripping and tearing food. Premolars and molars are situated to the back of the human mouth. These teeth are used for grinding and crushing food. This set of human teeth therefore increases the surface area of complex foods which in turn increases their chances of being exposed to digestive chemicals. Herbivorous mammal’s teeth are very different to that of humans. Premolars and molars are the main type of tooth in a herbivore. These types of teeth break down food by crushing and grinding. This aids herbivores as plant food contains cellulose which can only be broken down by excessive grinding and crushing. Therefore the role of the herbivores teeth is to break down plant material to increase the surface area of complex food which in turn exposes this food to digestive chemicals. Explain the relationship between the length and overall complexity of digestive systems of a vertebrate herbivore and a vertebrate carnivore with respect to: - the chemical composition of their diet - the functions of the structures involved Things to consider: What does explain mean? Simplify the question so that you understand what the question is asking Underline key words Use scientific terminology The digestive systems of vertebrate herbivores and vertebrate carnivores are very different. Herbivores eat plant material which contains cellulose. Cellulose is a good source of nutrients for herbivores. The problem for herbivores is that cellulose is broken down by the enzyme cellulase, which is not part of the herbivores digestive system. To overcome this problem herbivores have a symbiotic partnership with bacteria and protozoa. These microbes are present in the herbivores long caecum. The caecum provides a large surface area for bacterial enzymes to digest cellulose and to release the nutrients into the small intestine. The herbivore then digests the rest of their food. Kangaroo’s have two stomachs first stomach to digest cellulose. Ruminants large group of animals which have a four chambered stomach to digest cellulose: - 1st chamber the rumen moistens and churns up the food. - 2nd chamber the reticulum breaks down cellulose with bacterial cellulases and forms a round ball called a cud. - 3rd chamber omasum further digestion of the cud - 4th chamber abomasum acts as a stomach similar to that of any other mammal. Food passes to intestine for further digestion. Identify data sources, gather, process, analyse and present information from secondary sources and use available evidence to compare the digestive systems of mammals, including a grazing herbivore, carnivore and a predominantly nectar feeding animal Things to consider: - - What does identify, gather, analyse, present and compare mean? Simplify the question Be succinct Underline key words http://babcock.cals.wisc.edu/downloads/de_html/images/en1_digestion1.gif http://www.onedreamdesign.com/canine/pic/digestiveplate1cpy.jpg DOG COW Mostly feed on meat. Eating is rapid as well as the digestion of the meat Most of their time eating grass. Grass processed by a four chambered stomach. Main nutrient is cellulose which is broken down by cellulase HONEY POSSUM Mainly eat nectar and pollen Pollen containing protein, carbs and lipids. Nectar being sugary 2 chambered stomach where pollen and nectar are stored No caecum Specialised function is the tongue and roof of their mouth. Compare the roles of respiratory, circulatory and excretory systems Things to consider: - What does compare mean - Simplify the question - Use scientific terminology RESPIRATORY -This system consists of the lungs and the structures within the lungs to maintain our breathing - The main gases involved in respiration are called oxygen and carbon dioxide - The exchange of these gases are known as “gaseous exchange” - Therefore the main role of the respiratory system is to help organisms to breath as well as the exchange of gases in and out of the body CIRCULATORY - This system consists mainly of the heart and blood vessels - The heart pumps oxygenated blood around the body. - Deoxygenated blood returns to the heart. - Therefore the main role of the circulatory system is to pump blood around the body. EXCRETORY - This system consists mainly of the kidneys, urethra, ureter and the urinary bladder - The main function of the kidneys is to filter the blood. During filtration nutrients for the body are returned to the body while waste converts to the form of urine. - Waste passes into the ureter and then into the urinary bladder. - When the urinary bladder is full the urine is then passed out of the body. - Therefore the main function of the excretory system is to filter the blood through the kidney’s and to excrete waste as urine Identify and compare the gaseous exchange surfaces in an insect, a fish, a frog and a mammal Things to consider: - What does identify and compare mean? - Simplify the question so you know what it is asking - Be succinct - Use scientific language IDENTIFY COMPARE To perform gaseous The fish opens its mouth, lowering the buccal FISH exchange fish have cavity floor. The water flows in. Fish then gills. closes its mouth. Pressure of inside mouth increases. This pressure squeezes the water. The water then subsequently flows over the gills and out via the operculum. Fine tubes next to the capillary network pick up oxygen from the water while carbon dioxide is removed from the blood stream and out into the water. Gaseous exchange in The tracheae distribute oxygen to and collect INSECT an insect occurs carbon dioxide from body cells. This system through a set of tiny has the advantage of having a small surface holes called spiracles. area exposed to the atmosphere while having These spiracles are a large internal surface area for maximum gas connected to a network exchange. of tubes called tracheae. Gaseous exchange The skin of a frog has a well developed blood FROG occurs in two ways. supply. Oxygen from the air diffuses into the The skin and the lungs. skin and is transported by the blood to the heart, and sent all around the body. Gaseous exchange also occurs in the lungs whereby oxygen is passed in and out by the pumping movement of the buccal cavity and opening/closing of the nostrils Air passes through the mouth/nostrils down MAMMAL Gaseous exchange occurs through the the trachea and into the lungs. Alveoli lungs. provide large surface area for gas exchange. Oxygen diffuses from the alveoli into the blood stream. Carbon dioxide diffuses back into the lungs from the blood stream and is breathed out. Explain the relationship between the requirements of cells and the need for transport systems in multicellular organisms (pg 89, 90) Things to consider: - What does explain mean - What is the question asking? - Be succinct and use scientific terms Multicellular organisms need transport systems as they transport important nutrients such as amino acids, carbon dioxide and oxygen to cells. For example a transport system in the human body is the cardiovascular system. This system transports blood around the body. The blood contains oxygen. This oxygen is transported to cells. The oxygen enters the cells by diffusion. The oxygen is then used by the mitochondria to perform a process known as cellular respiration. Therefore it is evident that multicellular organisms need transport systems as they allow certain substances to travel to and from cells. Outline the transport system in plants including: (pg 86, 87, 88, 95, 96, 97) - root hair cells xylem phloem stomates and lenticels Things to consider: - What does outline mean? Underline key words and determine what they mean. Be succinct and use scientific terminology in your answer. Plants have many transport systems which aid in water absorption, nutrient absorption and gas exchange. The following structures are transport systems within plants; root hair cells, xylem, phloem, stomates and lenticels. Root hair cells are small structures within plants. They increase the surface area which therefore increases the absorption of water and minerals. Xylem are tube like structures. Xylem can be strengthened by a woody structure called lignin. Xylem transport water and dissolved mineral salts. The process starts in the root hairs. Water and mineral salts pass through the root hairs into root tissue and into the xylem. Water and mineral salts then pass through the xylem into the leaves where some of it is used for photosynthesis while most of the water is transpired. Phloem is a tube like structure that carries mainly glucose around the plant. Glucose can be converted into other substances such as starch and sucrose. The movement of nutrients within the phloem is called translocation. If a substance is to be returned to a previous location it is called retranslocation. Stomates and lenticels have a similar function within a plant. They are both involved in gas exchange. Stomates are tiny pores in the leaves which aid in the sucking up of water as well as releasing oxygen into the air and taking in carbon dioxide for photosynthesis. In larger plants there are also gaps in the bark which allows gas diffusion. These gaps are filled with parenchyma cells, which in turn are called lenticels. These lenticels allow easy diffusion of gases in and out of plants. Compare open and closed circulatory systems using one vertebrate and one invertebrate as examples. - What does compare mean? Underline key words Make sure you include a vertebrate and invertebrate as an example. VERTEBRATE Vertebrates have a closed circulatory system. This means that the blood stays within the blood vessels. Materials diffuse in and out of the blood vessels via the capillaries. The lymphatic system collects excess fluid from around the cells and transports it to the bloodstream. Lymphatic system is closed. INVERTEBRATE Invertebrates have an open circulatory system. Invertebrate’s blood is at low pressure and is pumped directly into the main cavity. Blood is collected by valves and drained back to the heart. The small size of invertebrates allows them to function with a relatively inefficient circulatory system. Use available evidence to discuss, using examples, the role of technologies, such as the use of radioisotopes in tracing the path of elements through living plants and animals. (Text book page 97. Dot point page 49, 50) Things to consider: - What does discuss mean? - Simplify the question to know all the terms in the question - Be scientific, key words etc Radioactive forms of certain elements can be utilised in the production of radioactive isotopes. These radioactive isotopes aid scientists as they are capable of tracing certain biochemical pathways. The radioactive isotope acts much the same as the nonradioactive isotope in the biochemical pathway to be tested. For example: Autoradiography is a technique used by scientists to trace the movement of certain substances around a plant. It involves the following steps: Carbon – 14 a radioactive isotope, is added to plant to observe the movement of carbon in the process of photosynthesis. The plant uses the radioactive isotope carbon – 14 in the process of photosynthesis. The movement of the carbon – 14 isotope can be traced by using an autoradiograph. This is similar to when we have an x – ray. The autoradiograph is produced by placing the plant against photographic film. The areas of the plant that are dark in nature is where carbon – 14 has built up. Other examples of radioisotopes include: Thallium – 201: Used to detect damaged heart tissue after a heart attack. The isotope will only accumulate in normal undamaged heart tissue. This enables doctors to determine how much of the heart tissue was damaged during the heart attack. Technetium – 99: This radioactive isotope emits gamma radiation and is useful in scintigraphy, (method used to examine organs to diagnose cancer and other diseases). USE OF THALLIUM – 201 (http://www.drdo.org/pub/techfocus/jun2000/image/401.jpg) USE OF TECHNETIUM – 99 (http://www.ndteducational.org/images/Brandenburg_Figure1.jpg) Perform a first-hand investigation of the movement of materials in xylem or phloem (This will be completed first lesson back) Things to consider: - Performing means? - Know how to write an aim, method, discussion, results, hypothesis etc AIM: To observe the movement of dye in the xylem and phloem of a piece of celery. HYPOTHESIS: I believe…..I think….. MATERIALS: Food dye 250ml beaker Celery Water METHOD: PART A 1. Pour approximately 100-150ml of water into a 250ml beaker. 2. Pour the same amount of water into another beaker, this time add five drops of food dye to the water. Add more dye if necessary. 3. Place a stalk of celery into the beaker with the plain water. 4. Place a stalk of celery into the beaker with the water and dye. 5. Leave the celery overnight. (Till Friday) PART B: 1. Take your celery and cut a cross section 2. Observe the xylem and phloem, and the movement of the dye. 3. Cut a thin section of the celery and prepare a wet mount slide of the celery and view under the microscope. 4. Draw your observations. RESULTS: Drawing of your specimen. Indicate the xylem, phloem and any other observations on your diagram. CONCLUSION: Write a conclusion to this experiment. Identify mitosis as a process of nuclear division and explain its role http://www.cellsalive.com/mitosis.htm Things to consider: - What does identify mean? - What does explain mean? - Know mitosis is nuclear division - Be succinct and use scientific language Mitosis is the duplication of chromosomal DNA followed by a cell division that results in two smaller daughter cells being formed, which are identical to the parent cell. Mitosis is an important process for all organisms. It is responsible for growth as well as tissue repair. Mitosis also plays an important role in asexually reproducing organisms. In this process mitosis transmits DNA codes from one cell generation to the next. Identify the sites of mitosis in plants, insects and mammals (Text page 104, 105, 106. Dot point book page 51) Things to consider: - What does identify mean? - Underline key words - Make sure you answers for plants, insects and mammals - Use scientific terminology Plants, insects and mammals are constantly undergoing mitosis in order for growth and repair of tissue. In plants mitosis occurs just behind the root cap, just below the shoot tip, buds along the stem and roots and in the cambium, which lies between the xylem and phloem. Insects have various stages in which they undergo mitosis. When a larva changes into a pupa, groups of cells form into discs. These discs are the sites for cell division and mitosis. New cells are produced here for growth and for the pupa to turn into an adult insect. Mammals undergo mitosis for growth and repair. The main sites for mitosis in mammals include the bone marrow and skin. Explain the need for cytokinesis in cell division (Text book page 103, 104. Dot point page 51) Cytokinesis is the division of the cytoplasm and occurs during the telophase stage of mitosis. In animal cells the cytoplasm constricts to the centre, breaks, and then forms into two cells. In plants there is no constriction. A cell plate is formed instead. The plate is a double membrane with a space between the two layers. The process of cytokinesis is important during mitosis as the cells stabilise the internal concentration of materials which is essential to produce two functioning cells. Identify that nuclei, mitochondria and chloroplasts contain DNA - Know the meaning of identify Simple question, answer should be short and straight to the point. Cells contain many organelles. These organelles enable the cell to function efficiently. The organelles that contain DNA are the nuclei, mitochondria and chloroplasts. Use available evidence to perform a first-hand investigation and gather first hand data to identify and describe factors that affect the rate of transpiration. Refer to aim, materials and method on page 116 of the text book. FACTORS AFFECTING THE RATE OF TRANSPIRATION Perform a first - hand investigation with a microscope to gather information from prepared slides to describe the sequence of changes in the nucleus of plant or animal cells undergoing mitosis. Refer to aim, materials and method on page 117 of the text book. IDENTIFICATION OF MITOTIC PHASES