EUROPEAN CURRICULUM VITAE FORMAT PERSONAL INFORMATION Name Address Telephone Fax E-mail Nationality Date of birth WORK EXPERIENCE EDUCATION AND TRAINING PERSONAL SKILLS AND COMPETENCES Irina M. Kuznetsova Tikhoretsky ave., 4, St. Petersburg, Russia +7(812) -297-1957 +7(812)-297-3541 imk@mail.cytspb.rssi.ru Russian 08.10.1954 Since 1978: Institute of Cytology- Postgraduate student, Junior Research Scientist, Research Scientist, Senior Research Scientist, Leading Research Scientist Since 1991: Lectures and practical training in biophysical study of proteins for the students of the State Polytechnical University, St.Petersburg, Russia. M.Sc. (Biophysics), 1978, Leningrad State University, USSR Ph.D. (Biology), 1982, Institute of Photobiology, Minsk, USSR Doct. Sci. (Biology) 2006, Institute of Cytology, St. Petersburg, RAS Protein folding and aggregation, amyloid fibrils, protein structure, conformation and dynamics, fluorescent dyes spectroscopy MOTHER TONGUE RUSSIAN OTHER LANGUAGES ENGLISH PROFESSIONAL AND Supervisor of 3 PhD students ORGANISATIONAL SKILLS AND COMPETENCES TECHNICAL SKILLS Biophysical method in proteins investigation AND COMPETENCES PATENTS no INVITED LECTURESHIPS AND ORAL PRESENTATION TO CONFERENCES PARTICIPATION TO COURSES PARTICIPATION TO CONFERENCES AND WORKSHOPS DRIVING LICENCE(S) ANNEXES Invited lectures: "Protein folding" Conference "Modern problems in polymer scince", 2006, St.Petersburg, Russia. "Thioflavin-T as an instrument for testing and investigation of amyloid and amyloid-like fibrils" International conference on coherent and nonlinear optics / International conference on lasers, applications, and technologies, 2007 Minsk, Belarus. • no • XIIth European Conference on the Spectroscopy of Biological Molecules. Bobigny, France (2007); International conference on coherent and nonlinear optics / International conference on lasers, applications, and technologies. Minsk, Belarus (2007); XXIX European congress on molecular spectroscopy. Opatija, Croatia (2008); XIIIth European Conference on the Spectroscopy of Biological Molecules. Palermo, Italy (2009) no Scientific Publications: Total Number: 26 As First/Last Author: 5 Total Impact Factor: 53.1 PUBLICATIONS: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Staiano M., Scognamiglio V., Rossi M., D'Auria S., Stepanenko Olga V., Kuznetsova I.M., Turoverov K.K. 2005. Unfolding-refolding of glutamine-binding protein from Escherichia coli and its stabilization by glutamine. Biochemistry 44, 5625-5633. Kuznetsova I.M, Stepanenko Olga V., Turoverov K.K., Scognamiglio V., Staiano M., Rossi M., and D'Auria S. 2005. Fluorescence properties of glutamine-binding protein from Escherichia coli and its complex with glutamine. J. Proteome Res. 4, 417-423. Bessonov A.N., Schagina L.V., Takemoto J.Y., Gurnev Ph.A., Kuznetsova I.M., Turoverov K.K., and Malev V.V.. 2006.Actin and Some Amphiphilic Polymers Influence on Channel Formation by Syringomycin E in Lipid Bilayers. Eur. Biophys. J. 35, 5, 382-392. Kuznetsova I.M., Turoverov K.K., Dunker A.K. and Uversky V.N. 2007. Analysis of folded, partially folded, and misfolded proteins with fluorescent dyes. In: Protein Structures: Methods in Protein Structure Analysis (Uversky V.N., Permyakov E.A., Eds.). Nova Science Publishers, Inc., Hauppauge, NY, USA (ISBN 1-60021404-5), Chapter 2.2, pp. 73-104. Turoverov K.K.,Kuznetsova I.M. Maskevich A.A., Stepuro V.I., Kuzmitsky V.A. and Uversky V.N. 2007. ThT as an instrument for testing and investigation of amyloid and amyloid-like fibrils. Proc. SPIE, 6733, 67331R, 1-11. Kuznetsova I.M., Povarova O., Stepanenko Olesya, Turoverov K.K., Crescenzo R., Varriale A., Staiano M., and D'Auria S. 2007. New perspectives in protein-based biosensors: The glucokinase from B. stearothermophilus and the odorant-binding protein from C. familiaris as probes for non-consuming analyte sensors. Proc. SPIE, 6733, 673318, 1-6. Maskevich A.A., Stsiapura V.I., Kuzmitsky V.A., Kuznetsova I.M., Povarova O.I., Uversky V.N. and Turoverov K.K. 2007. Spectral properties of thioflavin T in solvents with different dielectric properties and in fibril-incorporated form. J. Proteome Res. 6(4), 1392-1401 Povarova O.I., Kuznetsova I.M., Turoverov K.K. 2007. Different disturbances – one pathway of protein unfolding. Actin folding-unfolding and misfolding. Cell.Biol.Int. 31, 405-412. Stsiapura V.I., Maskevich A.A., Kuzmitsky V.A., Turoverov K.K., Kuznetsova I.M. 2007. Computational study of Thioflavine T torsional relaxation in the excited state J.Phys.Chem. A 111(22) 4829-4835. Svanidze A.V., Sashin I.L., Lushnikov S.G., Gvasaliya S.N., Turoverov K.K., Kuznetsova I.M., Kojima S. 2007. Inelastic incoherent neutron scattering in some proteins. Ferroelectrics, 348:154–160. Staiano M., D’Auria S., Varriale A., Rossi M., Marabotti A., Fini C., Stepanenko Olesya V., , Kuznetsova I. M., and Turoverov K.K. 2007. Stability and Dynamics of the Porcine Odorant-Binding Protein. Biochemistry 46(39):11120-7. Nevzglyadova O. V., Kuznetsova I. M., Artemov A. V., Mikhailova E. V., Turoverov K. K., and Soidla T. R. 2008. Comparative assay of amyloid and prion contents in yeast cells. 2(1): 71-80." Turoverov K.K., Kuznetsova I.M. and Uversky V.N. 2008. Parametric Dependence Method for the Analysis of Protein Folding. In Protein Structures: Methods in Protein Structure Analysis (Uversky V.N., Permyakov 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. E.A., Eds.) Nova Science Publishers, Inc., Hauppauge, NY, USA. (ISBN 1-60021-705-2), Chapter 4.2, pp. 257-318. Stepanenko Olesya V., Verkhusha V.V., Kuznetsova I.М., Uversky V.N. Turoverov К.К. 2008. Fluorescent proteins: Physical-chemical properties and and application in cell biology. Curr Protein Pept Sc. 9(4):338-69. Stepanenko Olesya V., Marabotti A., Kuznetsova I. M., Turoverov K.K., Fini C., Varriale A., Staiano M., Rossi M., and D’Auria S. 2008. The hydrophobic amino acid residues buried in the matrix of the porcine odorant-binding protein play an important role in protein stability and dynamics. Proteins. 71(1): 35-44. Stepanenko Olesya V., Verkhusha V.V., Shavlovsky M.M., Kuznetsova I.M., Uversky V.N. and Turoverov K.K. 2008. Understanding the Role of Arg96 in Structure and Stability of Green Fluorescent Protein. Proteins 73(3): 539-551. Stsiapura V.I., Maskevich A.A., Kuzmitsky V.A., Uversky V.N., Kuznetsova I.M., Turoverov K.K., 2008. Thioflavin T as a molecular rotor: fluorescent properties of thioflavin T in solvents with different viscosity. J.Phys.Chem. B . 112(49) 15893-15902. Nevzglyadova O. V., Artemov A. V., Mittenberg A.G., Solovyov K. V., Kostyleva E.I., Mikhailova E. V., Kuznetsova I. M., Turoverov K.K., Soidla T.R. 2009. Prion-associated proteins in yeast: comparative analysis of isogenic [PSI+] and [psi-] strains. Yeast 26: 611–631. Alexander V. Fonin, Olga V. Stepanenko, Irina M. Kuznetsova, Konstantin K. Turoverov, Elena I. Kostyleva, Vladimir I. Vorobyev. 2010. Interaction between linker histone H1 and non-histone chromatin protein HMGB1. Spectroscopy. Biomedical Applications. 24, 1-2: 165-168. Olga V. Stepanenko, Olga I. Povarova, Olesya V. Stepanenko, Alexander V. Fonin, Irina M. Kuznetsova, Konstantin K. Turoverov, Maria Staiano, Sabato D’Auria. 2010. Structure and stability of D-galactose/Dglucose-binding protein. The role of D-glucose binding and Ca ion depletion. Spectroscopy. Biomedical Applications. 24,3-4: 355-359. Olesya V. Stepanenko, Irina M. Kuznetsova, Vladislav V. Verkhusha, Maria Staiano, Sabato D’Auria, Konstantin K. Turoverov. 2010. Denaturation of proteins with beta-barrel topology induced by guanidine hydrochloride. Spectroscopy. Biomedical Applications. 24,3-4: 367-373. Olga I. Povarova, Olga V. Stepanenko, Anna I. Sulatskaya, Irina M. Kuznetsova, Konstantin K. Turoverov, Maria Staiano, Annalisa Vitale, Sabato D'Auria. 2010. High stability of trehalose/maltose binding protein from Thermococcus litoralis makes it a good candidate as a sensitive element in biosensor systems for sugar control. Spectroscopy. Biomedical Applications. 24,3-4: 349-353. Nevzglyadova O. V., Artemov A. V., Mittenberg A. G., Mikhailova E. V., Kuznetsova I. M., Turoverov K. K. and Soidla T. R. 2010. Yeast Protein Aggregates, Containing Chaperones and Glucose Metabolism Enzymes. Chapter 6. In: Handbook of Molecular Chaperones: Roles, Structures and Mechanisms (P. Durante and L. Colucci, Eds), Nova Science Publishers, Hauppauge, NY. pp. 241-270. A.I.Sulatskaya, K.K. Turoverov, I.M. Kuznetsova. 2010. Spectral properties and factors determining high quantum yield of thioflavin T incorporated in amyloid fibrils. Spectroscopy. Biomedical Applications. 24,1-2: 169-172. Turoverov KK., Kuznetsova IM and Uversky VN. 2010.The protein kingdom extended: native globular and native partially or completely disordered proteins, their folding, supramolecular complex formation, and aggregation. Progress in Biophysics and Molecular Biology. 102(2-3): 73 - 84. A.I. Sulatskaya, I.M. Kuznetsova, A.A. Maskevich, V.N. Uversky and K.K. Turoverov. 2010. Non-radiative deactivation of the excited state of thioflavin T: Dependence on solvent viscosity and temperature. PlosOne (In press).