國立彰化師範大學九十四學年度碩士班招生考試試題
系所：生物技術研究所
科目： 分子生物學
☆☆請在答案紙上作答☆☆
共 5 頁，第 1 頁
請將答案寫在答案紙上，並正確標上題號。題號標錯不予計分。
I. 問答題
1.
A student used the firefly luciferase as a reporter gene to demonstrate the promoter function of X gene. She
isolated the X gene promoter with 982 bp in front of the start site of transcription (+1). To define the
sequence involved in the regulation of X gene, she made a series deletions containing various lengths of the
5’ regulatory region and transfected into mammalian cells. The reporter gene activity in the absence (-) and
presence (+) of metal ion were assay and the results were showed in above figure. Read carefully and
answer following questions. (2 % each)
(1). How to map the start site of transcription (+1) of the X gene?
(2). Assuming that there is a single metal responsive element (MRE) in the X gene, which fragment should
involved? Explain why?
(3). If you get the MRE sequence, how would you confirm your data by other experiments?
(4). Please write at least two more reporter genes except luciferase and give the full name.
(5). How the student constructed those various lengths of the 5’ regulatory region to the reporter gene?
(6). According to the above figure, please write down the results that you have found by yourself for best
discussion, but with no more than 100 words.
-1-
國立彰化師範大學九十四學年度碩士班招生考試試題
系所：生物技術研究所
科目： 分子生物學
☆☆請在答案紙上作答☆☆
共 5 頁，第 2 頁
2. What is the Rapid Amplification of cDNA End (RACE)-PCR for gene cloning? (3 %)
II. 選擇題: (2% each)
1. Which of the following method Cannot detect mRNA?
(a) Northern blot.
(b) Whole-mount in situ hybridization.
(c) Real-time PCR.
(d) RT-PCR.
(e) Gel-shift assay.
2. Which of the following types of information Cannot be determined from the Northern blotting?
(a) The relative levels of an mRNA species in different tissues.
(b) The half-life of an mRNA species.
(c) The size of an mRNA species.
(d) The strand of DNA that is transcribed into mRNA.
(e) The amino acid sequence of the protein coded by an mRNA species.
3. Which of the following about the ribosome involved is NOT true?
(a) aminoacylation of tRNA.
(b) binding of protein factors during elongation.
(c) peptide bond formation.
(d) binding of aminoacyl tRNA to mRNA.
(e) binding of mRNA at initiation codon.
4. Labeling of mesoderm with vital dyes in vertebrate embryo would result in labeling of which of the
following adult tissues? I. Notochord. II. Red blood cells. III. Neural.
(a) I only.
(b) II only.
(c) III only.
(d) I and II.
(e) II and III.
(f) I, II, and III.
5. In the classical model of transcriptional control described by Jacob and Monod, a repressor protein binds
to
(a) a TATA box.
(b) terminator.
(c) AUG sequence.
(d) an operator.
(e) a ribosomal-binding site.
-2-
國立彰化師範大學九十四學年度碩士班招生考試試題
系所：生物技術研究所
科目： 分子生物學
☆☆請在答案紙上作答☆☆
共 5 頁，第 3 頁
6. Which is NOT contributed to promoter binding by RNA polymerase in E. coli?
(a) rho factor.
(b) TATA box.
(c) -35 consensus sequence.
(d) βsubunit of RNA polymerase.
(e) β’subunit of RNA polymerase.
7. When the DNA-binding domain of a glucocorticoid receptor protein is replaced with the DNA-binding
domain of the estrogen receptor protein, the hybrid regulatory protein activate Which genes in response to
glucocorticoid.
(a) Glucocorticoid responsive genes.
(b) Estrogen responsive genes.
(c) Both glucocorticoid and estrogen responsive genes.
(d) Neither glucocorticoid nor estrogen responsive genes.
(e) None genes are activate.
8. Which is True about the function of the sigma subunit in the RNA polymerase of E. coli?
(a) It is belong to the core enzyme of RNA polymerase.
(b) It is essential for elongation of the RNA transcript.
(c) It is recognize specific binding site to promoter sequence.
(d) It is require for transcription termination.
(e) It is require for translation.
9. Which is NOT true in the processing of mRNA precursors in eukaryotic cells ?
(a) Addition of poly A.
(b) Capping of the 5’ end.
(c) Excision of introns.
(d) Transport of the pre-mRNA to the cytoplasm.
(e) Splicing of exons.
10. Which of the following is NOT a DNA-binding domain?
(a) The steroid receptor.
(b) The Zinc finger motif.
(c) The helix-loop-helix motif.
(d) Greek key β-barrel domain.
(e) The homeodomain.
(f) Leucine zippers.
-3-
國立彰化師範大學九十四學年度碩士班招生考試試題
系所：生物技術研究所
科目： 分子生物學
☆☆請在答案紙上作答☆☆
共 5 頁，第 4 頁
III.
1.
What are the advantages of using “secretion system” to express proteins?
(1)
(3%)
(2)
(3%)
2.
What is the disadvantage of using polyethylene glycol to precipitate proteins?
(3)
how to solve this problem?
(4)
(3%)
(3%)
3.
What are the disadvantages of using “Retroviral Vector Method” to generate transgenic animals?
(5)
(3%)
(6)
(3%)
4.
What are possible problems for making protein array?
(7)
(3%)
(8)
(3%)
5.
How to make vaccines against antigens which are not proteins? (6%)
IV. 選擇題: (2% each)
1. The sequence of one strand of DNA is ATGATTACA, and that of RNA transcribed from the DNA is
AUGAUUACA. So the sequence of DNA is NOT called a) coding strand; b) anti-sense strand; c) plus
strand; d) partner strand.
2. The sequence of RNA is AUGAUUACA. What is the sequence of its DNA template? a) ATGATTACA;
b) TACTAATGT; c) TGTAATCAT; d) ACATTAGTA.
3. The following sequence UAGDCGGDAG belongs to a) mRNA; b) tRNA; c) rRNA; d) snRNA.
4. Which of the following is NOT part of large subunit of ribosomes in mammals? a) 18S rRNA; b) 5S
rRNA; c) 28S rRNA; d) 5.8S rRNA.
5. Which of the following RNAs is involved in directing modificaton of rRNAs? a) snRNA; b) snoRNA; c)
siRNA; d) stRNA.
6. What is the right sequence of the conformations of chromotin fibers from least condensed to most
condensed ? a) chromosome→solenoid→beads-on-a-string→nucleosome; b) nucleosome→
beads-on-a-string→solenoid→chromosome; c) chromosome→beads-on-a-string→solenoid→
nucleosome; d) nucleosome→solenoid→beads-on-a-string→chromosome.
-4-
國立彰化師範大學九十四學年度碩士班招生考試試題
系所：生物技術研究所
科目： 分子生物學
☆☆請在答案紙上作答☆☆
共 5 頁，第 5 頁
7. What is the protein to be the backbone of a condensed chromosome? a) histones; b) G proteins; c)
chromotin proteins; d) scaffold proteins.
8. Which of the following is NOT true in retroviruses? a) their genomes are ssRNA ; b) they integrate into
host genome in the form of dsDNA; c) the only function of their reverse transcriptase is to
reverse-transcribe RNA into cDNA; d) LTRs comprise a transcription start site as well as a polyA site.
9. a) methylation of cytosine bases; b) binding of activator to enhancer; c) binding of RNA polymerase to
promoter; d) histone acetylation is NOT the requirement of expression of a gene.
10. If a protein is made to be localized in Golgi apparatus, which of the following is NOT present in the
protein? a) hydrophobic N terminus, b) KDEL in C terminus, c) a specific transmembrane  helix, d)
mannose-6-phosphate.
11. What is the wobble position during protein synthesis? a) the first position of a codon; b) the first
position of an anticodon; c) the first position of a codon; d) none of above.
12. Which of the following is NOT involved in the initiation of protein synthesis in E. coli? a) mRNA; b) IF;
c) tRNAMet; d) ribosomes.
13. Which of the following is NOT a transposable element? a) P element; b) Ty; c) SINEs; d) Alphoid.
14. The synthesis of peptide in protein synthesis is catalyzed by a) terminal transferase, b) peptidyl
transferase, c) elongation factor, d) translocase.
15. Telomere is a type of a) satellite DNA; b) minisatellite DNA; c) microsatellite DNA; d) interspersed
repeats.
V.
You were a molecular biologist and isolated DNA from E. coli. The reading of OD260 of the isolated DNA
was 1.200. Then you performed a PCR (polymerase chain reaction) to amplify a segment of DNA. The
following is the experimental reaction mix you designed. What is the answer of the X and Y? (5%)
Vol (l)
Final concentration
10 x buffer
5
dNTPs
4
100 M
Primer1
2
10 M
Primer2
2
10 M
DNA
X
200 ng
Taq polymerase
0.5
2.5 units
dH2O
Y
Total vol.
50
-5-