Table 1. Sexes of progeny following crosses of S. menziesii and S. salicaria. All females of
S. menziesii (categorized by phenotype) were crossed to heterozygous hermaphrodites of S.
salicaria (Hh) derived from control lines of a quantitative genetics experiment (Sakai et al.
2008). Heterozygosity of hermaphrodites of S. salicaria was previously confirmed (crosses to
females of S. salicaria [hh] segregated females and hermaphrodites). Individuals of S.
menziesii used in crosses were designated by the field population number (vouchers at US),
the ID of the tagged field plant from which seeds were collected, and the ID of progeny
derived from the field plant. Hermaphroditic individuals of S. salicaria were designated by
the parental population, the family ID from a partial diallel cross, and the ID of progeny of the
plant within the family. Each maternal parent was crossed to two different paternal parents.
A. Crosses of S. menziesii and S. salicaria that segregated both females and hermaphrodites.
G values testing a 1:1 ratio of progeny were not significant, with one exception (* P<0.001).
B. Sexes of progeny following crosses of S. menziesii and S. salicaria that segregated only
hermaphrodites or nearly all hermaphrodites.
A. Crosses typically segregating approximately equal numbers of females and
hermaphrodites.
S. menziesii
S. salicaria
Female
Hermaphroditic
Value for G
female parent hermaphrodite used
progeny
progeny
as male parent
849-17-3
842-52-15
18
12
0.12081
849-17-3
842-32-6
14
17
0.2908
849-17-13
842-60-12
11
18
1.7065
849-17-13
842-9-6
14
15
0.0345
849-42-11
842-4-17
11
19
2.1494
849-42-11
842-133-22
13
15
0.1430
849-42-23
842-32-18
15
16
0.0323
849-42-23
842-2-2
14
15
0.0345
849-42-28
842-52-15
5
4
849-42-28
842-149-5
4
3
849-72-14
842-4-17
12
17
0.8664
849-72-14
849-57-39
849-57-39
950-407-2
950-407-2
842-2-2
842-141-6
842-9-6
842-163-23
842-225-3
16
14
17
0
8
12
16
12
29
19
0.5734
0.1334
0.8664
40.203*
2.3073
Female
progeny
Hermaphroditic
progeny
G
0
0
0
0
0
0
0
1
0
1
0
0
0
0
2
0
0
0
0
0
0
0
0
0
0
0
28
30
7
30
29
29
29
28
30
30
30
30
17
15
29
29
30
31
30
29
30
30
30
27
29
27
-
B. Crosses typically segregating only hermaphrodites.
S. menziesii
female parent
849-93-4
849-93-4
849-93-12
849-93-12
849-93-16
849-93-16
849-93-21
849-93-21
849-93-27
849-93-27
849-93-30
849-93-30
950-407-1
950-407-1
950-422-2
950-422-2
950-428-10
950-428-10
950-435-1
950-435-1
950-444-3
950-444-3
950-501-10
950-501-10
950-501-28
950-501-28
S. salicaria
hermaphrodite used
as male parent
842-141-6
842-74-13
842-163-23
842-2-2
842-27-4
842-95-16
842-196-22
842-225-3
842-128-16
842-133-22
842-60-12
842-32-6
842-33-18
842-144-15
842-196-22
842-149-5
842-163-23
842-144-15
842-27-4
842-76-10
842-5-1
842-95-16
842-5-1
842-2-2
842-128-16
842-74-13
Table 2. Crosses between Schiedea salicaria and plants from the hybrid zone. A. Sexes of
progeny following crosses of female Schiedea salicaria and paternal hermaphroditic plants from
the hybrid zone. For those crosses that segregated females and hermaphrodites, G tests were
based on an expected 1:1 segregation. One hybrid (902-108-1) was crossed as a paternal parent
to two different S. salicaria females. G values testing a 1:1 ratio of progeny were not significant,
with one exception (* P<0.001). B. Sexes of progeny following crosses of a hybrid plant used
as a female, and two S. salicaria hermaphrodites heterozygous at the sex-determining locus used
as male parents. † indicates a possible case of contamination (1 female in a total of 202
progeny).
Female parent
Male parent
Females
Hermaphrodites Percent
Value for G
(N)
(N)
flowering
A. Crosses using hermaphroditic plants from hybrid zone as male parents
S. salicaria
Hybrid
50
45
95
0.2633
(C2 638-1F)
(902-108-2)
S. salicaria
Hybrid
51
48
99
0.0909
(C1 629-4F)
(902-108-4)
S. salicaria
Hybrid
32
74
97.25
17.11*
(C2 633-4F)
(902-116,
cutting of field
plant)
S. salicaria
Hybrid
44
54
98
1.0222
(C1 649-16F) (902-116-3)
S. salicaria
Hybrid
0
98
98
(C1 612-11F) (902-108-1)
S. salicaria
Hybrid
0
100
99.01
(C1 605-12F) (902-108-1)
S. salicaria
Hybrid
0
99
100
(C1 611-1F)
(902-111-2)
S. salicaria
Hybrid
0
99
99
(C2 653-14F) (902-111-7)
S. salicaria
Hybrid
0
100
100
(C1 620-14F) (902-111-8)
B. Crosses using an apparent female plant from the hybrid zone
Hybrid
S. salicaria
1†
103
100
(902-111-3H) (C2 633-14H)
Hybrid
S. salicaria
0
98
98
(902-111-3H) (C1 611-7H)