SUPPORTING INFORMATION For The Magnesium Transporter 5 Plays an Important Role in Mg Transport for Male Gametophyte Development in Arabidopsis Xiao-Feng Xu, Bo Wang, Yue Lou, Wen-Jian Han, Jie-Yang Lu, Dan-Dan Li, Le-Gong Li, Jian Zhu*, Zhong-NanYang* *To whom correspondence should be addressed. E-mail: znyang@shnu.edu.cn (ZN.Y); zhujian1@tongji.edu.cn (Z.J). This file including Table S3. Transmission efficiency of the heterozygous mutant of mgt9 and identification of MGT9+/- progeny via self-pollination under high Mg condition. Table S3. Transmission efficiency of the heterozygous mutant of MGT9 and identification of MGT9+/- progeny via self-pollination under high Mg condition. Parental Genotypes Male WT MGT9+/a MGT9+/- Female MGT9+/WT a MGT9+/- Genotypes of Progeny MGT9+/+ 114 221 148 MGT9+/116 0 150 mgt9-/0 0 0 Total TEf TEm 230 221 298 50.43% NA NA NA 0 NA The transmission efficiencies (TE) were calculated according to the following equation: TE = number of progenies with T-DNA insertion/number of total progeny × 100%. For normal transmission, 50% reciprocal crosses were expected. TEF, female transmission efficiency; TEM, male transmission efficiency; NA, not applicable. a, heterozygous mutant of MGT9 were grown in 10 mM Mg condition.