Suh et al. – Supplementary material
Supplementary Material
Suh et al. - Inter-population variation for Wolbachia induced reproductive incompatibility in
the haplodiploid mite Tetranychus urticae
Contents
Table S1 ......................................................................................................................... 2
Table S2 ......................................................................................................................... 3
Figure S1 ........................................................................................................................ 4
Figure S2 ........................................................................................................................ 5
1
Suh et al. – Supplementary material
Table S1. Difference of host plant and geographical area between group W1 and W2
categorized based on difference in partial sequences of wsp genes.
Group
Host plant
Rose
Population
DJ
JJ
Red pepper
GN
W1
SC
Cherry tomato
YS
Pumpkin
HS1
IC
Chrysanthemum
HS2
MS
Red pepper
BY
W2
JE
YJ
Rose
SW
JC
2
Suh et al. – Supplementary material
Table S2. Comparison in wsp gene sequences of field-collected Tetranychus urticae
populations and PR strain or groups categorized based on difference in partial sequences of
wsp genes.
Host population or
No. of analyzed wsp gene
No. of different wsp
Max genetic
Wolbachia group
sequence (clone)
gene sequence
distance
DJ
3
3
0.006
JJ
2
1
0.000
YS
2
1
0.000
GN
2
2
0.002
HS1
2
2
0.004
SC
2
1
0.000
Group W1
13
7
0.006
IC
1
1
0.000
SW
3
3
0.004
JC
5
3
0.004
BY
3
1
0.000
JE
2
1
0.000
HS2
4
3
0.004
YJ
2
2
0.004
S
4
3
0.004
PR
2
1
0.000
Group W2
26
10
0.006
39
17
0.013
Group W1
and W2
3
Suh et al. – Supplementary material
Figure S1. Multiple alignment of nucleotide sequences of the wsp gene of Tetranychus
urticae populations generated using the DNAMAN package (Lynnon Biosoft, Quebec,
Canada). Gaps(-) were introduced for the same nucleotide. Sequence ID and sequence
information from W1 group were shaded, and the diagnostic nucleotide sequences that
categorize W1 and W2 were boxed.
4
Suh et al. – Supplementary material
Figure S2. Consensus nucleotide sequences (top), and amino acid sequences (bottom) of
group A and B categorized based on sequential difference. Consensus sequences were
generated by DNAMAN package (Lynnon Biosoft, Quebec, Canada). Amino acids sequences
were constructed by DNA translation of DNAMAN package with frame 2 option. Gaps (-)
were introduced for the same nucleotide or amino acid. The diagnostic nucleotide sequences
that categorize W1 and W2 were shaded and the resulting change in amino acid sequence was
boxed.
5