DANAGENE DNA/RNA
PURIFICATION Kit
Selective isolation of DNA and RNA from unique,
precious samples is a demand for an increasing
number of applications. Splitting is unfavourable if
not impossible for a number of samples like
biopsy samples, single foci from cell cultures or
single individuals of small organisms.
DANAGENE DNA/RNA PURIFICATION Kit
provides a rapid method for the isolation and
purification genomic DNA and total RNA
simultaneously from a single sample of cultured
animal cells, small tissues samples, blood, bacteria,
yeast, fungi or plants.
The process involves first lysing the cells o tissue
of the interest in a highly denaturing guanidineisothiocyanate-containing buffer that will rapidly
inactive RNases and DNases to ensure isolation of
intact DNA and RNA. The lysate is then passed
throuhg a DNA spin column, this column allows
selective binding of genomic DNA. The column is
washed and pure, ready-to-use DNA is the eluted.
Ethanol is added to the flow-through from the
DNA spin column to provide appropriate binding
conditions for RNA, and the sample is the applied
to a RNA spin column, where total RNA binds to
the membrane and contaminants are efficiently
washed away, high-quality RNA is then eluted.
Total RNA
genomic DNA
The kit purifies all sizes of RNA, from large
mRNA and ribosomal RNA down to microRNA
and small interfering RNA. The purified RNA can
be used in a number of downstream applications
including real time PCR, RT-PCR, Northern
blotting, RNase protection and primer extension
and expression array assays. The genomic DNA is
of the highest quality and can be used in PCR
reactions, sequencing, Southern blotting and SNP
analysis.
FEATURES
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High quality DNA and RNA from the
same sample using DNA and RNA spin
columns.
Analysis will be more reliable since the
RNA and DNA are derived from the
same sample.
DNA and RNA are isolated without the
use of harmful chemicals as phenol or
chloroform.
Fast and rapid processing in less than
20 minutes.
Ready-to-use DNA and RNA for any
downstream analysis.
Simultaneous isolation of total RNA and
genomic DNA from HeLa Cells.
Panel A show the RNA that was isolated from 2
different samples of HeLa cells.
Panel B show the genomic DNA that was isolated
from the same 2 HeLa cell samples.
DANAGENE DNA/RNA
PURIFICATION Kit
Ref.0805
Allows to isolate genomic DNA and total RNA
from the same sample. 50 tests.